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OfflineElixier
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Registered: 11/16/08
Posts: 287
Last seen: 15 years, 5 months
Super Spawning
    #10620806 - 07/04/09 12:45 PM (15 years, 6 months ago)

June 4th, '09

Starting with a colonized montotub I broke it up and added an additional 2 quarts of colonized grain to it.  This will later act as the spawn to an even larger substrate.  I'm not going to cover the process I used to make the monotub because its already well documented.  However, 5 quarts to one block of coir is what I did.  The ultimate goal is to move this to an outdoor bed and cover with 80 lbs of compost/manure.



Since I will not be pasteurizing my final substrate, I did an acclimation process similar to immunity shots humans receive.  A small amount of unpasteurized substrate is added to the spawn so the mycelium will be prepared to fight against bacteria and other competitors once I super spawn it.  For my substrate I'm using a compost/manure blend acquired at a home improvement store. 



Note that I mixed in the compost/manure and did no pasteurization to it.  In order to prevent the exposed grain on top from drying out I put a layer of wax over the whole thing and am planning on letting this sit for a few days. 




Questions, comments, and criticism appreciated, updates to follow.

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OfflineLennyk
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Re: Super Spawning [Re: Elixier]
    #10620903 - 07/04/09 01:04 PM (15 years, 6 months ago)

Quote:

Elixier said:
June 4th, '09

Starting with a colonized montotub I broke it up and added an additional 2 quarts of colonized grain to it.  This will later act as the spawn to an even larger substrate.  I'm not going to cover the process I used to make the monotub because its already well documented.  However, 5 quarts to one block of coir is what I did.  The ultimate goal is to move this to an outdoor bed and cover with 80 lbs of compost/manure.







Why did you add more spawn when it was fully colonized?

Quote:

Since I will not be pasteurizing my final substrate, I did an acclimation process similar to immunity shots humans receive.  A small amount of unpasteurized substrate is added to the spawn so the mycelium will be prepared to fight against bacteria and other competitors once I super spawn it.  For my substrate I'm using a compost/manure blend acquired at a home improvement store.




Where did you get the idea this works? I mean, I never have ever read or heard about anything like this. 

][
Quote:

url=https://files.shroomery.org/files/09-27/673239959-acclimation.jpg][/url]

Note that I mixed in the compost/manure and did no pasteurization to it.  In order to prevent the exposed grain on top from drying out I put a layer of wax over the whole thing and am planning on letting this sit for a few days.





Questions, comments, and criticism appreciated, updates to follow.





To prevent drying out, grains should always be covered in the bulk substrate, the frosting layer stuff is wrong. You don't want exposed grains. (And I don't see why you don't pasteurize substrate that I am sure has tons of trich spores) 

Frankly I think your project is going to get very green, very quickly  :omgz:


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OfflineElixier
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Registered: 11/16/08
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Last seen: 15 years, 5 months
Re: Super Spawning [Re: Lennyk]
    #10620930 - 07/04/09 01:10 PM (15 years, 6 months ago)

I added 2 more quarts of spawn to increase colonization speed of my final substrate.  The idea was acquired from Paul Stamets' latest book, "Mycelium Running" in chapter nine.  As for trichodermia, the initial substrate (the coco coir, which is going to be used as spawn for the final substrate) is already colonized so it will be able to hold its own against a competitor such as trichodermia.  Finally after a day or so of the acclimation process I intend to move it to an outdoor bed where high levels of fresh air will further inhibit trichodermia.

Or my experiment could go wrong and it will all be fucked, but that's how you learn.

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Invisibleprismism
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Registered: 05/11/09
Posts: 5,570
Re: Super Spawning [Re: Elixier]
    #10620931 - 07/04/09 01:11 PM (15 years, 6 months ago)

fail or not..im quite interested in your results.


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OfflineLennyk
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Re: Super Spawning [Re: Elixier]
    #10620976 - 07/04/09 01:17 PM (15 years, 6 months ago)

Quote:

]Elixier said:
I added 2 more quarts of spawn to increase colonization speed of my final substrate.  The idea was acquired from Paul Stamets' latest book, "Mycelium Running" in chapter nine


.

Haven't read the book, but I don't see why spawn would be added at that point instead of at the first time of spawning, or at the point of when it is "superspawned", which I guess happens in nature with manure to manure and stuff, but as far as I knew when it happens in nature the yields are minimal and can takes months or years when it simply mother nature at work.

 
Quote:

As for trichodermia, the initial substrate (the coco coir, which is going to be used as spawn for the final substrate) is already colonized so it will be able to hold its own against a competitor such as trichodermia.  Finally after a day or so of the acclimation process I intend to move it to an outdoor bed where high levels of fresh air will further inhibit trichodermia.

Or my experiment could go wrong and it will all be fucked, but that's how you learn.





If fully colonized material prevented the problems of trich one would never have to pasteurize their material with a high spawn rate, but that is not the case. The outdoors defiantly helps against trich, but I have a feeling it will defiantly begin enough establishment indoors in the "acclimation". Trich works quickly.

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OfflineElixier
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Registered: 11/16/08
Posts: 287
Last seen: 15 years, 5 months
Re: Super Spawning [Re: Lennyk]
    #10621013 - 07/04/09 01:24 PM (15 years, 6 months ago)

Meh, I just had a couple of extra jars laying around.  As for nature yields are minimal, take a look for yourself.

http://www.shroomery.org/forums/postlist.php/Board/61

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OfflineRJ Tubs 202
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Registered: 09/20/08
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Re: Super Spawning [Re: Elixier]
    #10621199 - 07/04/09 01:57 PM (15 years, 6 months ago)

The main worry I'd have about expanding spawn to a non-pasturized substrate is FAE, because I think this factor is extra critical for this type of bulk approach.

Another idea would be to go from coir to a small outdoor bed, and then expanding the small bed into a larger one.

Good luck.

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OfflineLennyk
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Re: Super Spawning [Re: Elixier]
    #10621396 - 07/04/09 02:53 PM (15 years, 6 months ago)

Quote:

Elixier said:
Meh, I just had a couple of extra jars laying around.  As for nature yields are minimal, take a look for yourself.

http://www.shroomery.org/forums/postlist.php/Board/61





I mean nature without the aid of humans...

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