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Lostkeys
Fractal Thought Pattern



Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 1 year, 1 month
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Re: How do these look? (first grow) [Re: JMath]
#10610464 - 07/02/09 03:27 PM (15 years, 6 months ago) |
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Did you shake up your syringe? Most of the ones I've seen have a giant clump of spores in there that you gotta shake up to mix evenly throughout to the syringe. If you didn't do this, you could have shot your whole load into the first inoculation point... That's just the frist thing that came to mind.
-------------------- So heavy I fell through the earth...

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JMath
Stranger

Registered: 06/06/09
Posts: 120
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Re: How do these look? (first grow) [Re: Lostkeys]
#10610507 - 07/02/09 03:35 PM (15 years, 6 months ago) |
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Nope definitely shook it up, didn't seem to be a ton of spores in there but enough to get some in at least more than one jar. I actually shook it between every jar.
Edited by JMath (07/02/09 03:38 PM)
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JMath
Stranger

Registered: 06/06/09
Posts: 120
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Re: How do these look? (first grow) [Re: JMath]
#10611709 - 07/02/09 07:33 PM (15 years, 6 months ago) |
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I also feel like it might be that I didn't let the jars cool long enough. The first five sterilized were cooled for about five hours, but the second batch only cooled for a little over three hours. Now I know you're supposed to wait 12 hours, but they were cool to the touch, and when you inoculate you squirt the solution down the sides of the glass so if the glass is cool to the touch then wouldn't the substrate on the outside where you inject be cool also? Even though the core may still be hot, the outside would be cool, does that make sense?
And even if it were the heat, how does that explain the fact that one jar is colonizing great? I don't know, I guess I'll just be happy that I got one jar and go ahead and start a new batch (after I wait another week of course).
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: How do these look? (first grow) [Re: ylibniquah]
#10612119 - 07/02/09 09:05 PM (15 years, 6 months ago) |
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Quote:
ylibniquah said:
Quote:
Stealthgrower said:
Quote:
JMath said: raised the temp to about 80 to see if I could get faster growth
While you're at it, go ahead and put up a sign near the jars that says, "come on in bacteria, I got just the place for you to flourish, right here, in these jars"
ha, yeha, 80 is too high. 70-75
80 is not too high! Where you coming up with this? 80-81 is optimal! Not necessary but optimal! If properly done there will be no bacterial infection even at 90F and if not done right bacteria will flourish regardless if it's 75 or 80! Think about it!!!
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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ylibniquah
Innerspace Explorer



Registered: 05/05/09
Posts: 236
Loc: GA (unfortunately!)
Last seen: 3 years, 5 months
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Re: How do these look? (first grow) [Re: hyphae]
#10617845 - 07/03/09 08:33 PM (15 years, 6 months ago) |
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70-75 is optimal, as stated by RR. I have worked very hard to keep my temps under 75, and I can say that it does produce great results. Take up your differences in opinion with him. I've seen him say it a million times, and I'm sure he has a good reason to say it. At any rate, 70-75 not only is optimal, but it's the comfortable temp range for ppl in their homes.... 80 would require an incubator for your jars, unless you keep your home on the hot side. there's a lot of misinformation on this board, but after watching RR's vids, I would take his advice on anything mycology related.
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Psuper
Psilocybin

Registered: 02/12/08
Posts: 2,878
Loc: PNW
Last seen: 1 month, 1 day
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Re: How do these look? (first grow) [Re: ylibniquah]
#10617880 - 07/03/09 08:42 PM (15 years, 6 months ago) |
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Quote:
ylibniquah said: 70-75 is optimal, as stated by RR. I have worked very hard to keep my temps under 75, and I can say that it does produce great results. Take up your differences in opinion with him. I've seen him say it a million times, and I'm sure he has a good reason to say it. At any rate, 70-75 not only is optimal, but it's the comfortable temp range for ppl in their homes.... 80 would require an incubator for your jars, unless you keep your home on the hot side. there's a lot of misinformation on this board, but after watching RR's vids, I would take his advice on anything mycology related.
Your missing a few points in your argument. Hyphae is right, the information your quoting is meant to reduce the risk of contams growing in the jars. Chill. ~Pixie~
-------------------- Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk
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CH HELL
Brain Sturgeon



Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: How do these look? (first grow) [Re: Psuper]
#10618041 - 07/03/09 09:24 PM (15 years, 6 months ago) |
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Quote:
Psuper said:
Quote:
ylibniquah said: 70-75 is optimal, as stated by RR. I have worked very hard to keep my temps under 75, and I can say that it does produce great results. Take up your differences in opinion with him. I've seen him say it a million times, and I'm sure he has a good reason to say it. At any rate, 70-75 not only is optimal, but it's the comfortable temp range for ppl in their homes.... 80 would require an incubator for your jars, unless you keep your home on the hot side. there's a lot of misinformation on this board, but after watching RR's vids, I would take his advice on anything mycology related.
Your missing a few points in your argument. Hyphae is right, the information your quoting is meant to reduce the risk of contams growing in the jars. Chill. ~Pixie~
Hyphea is correct, I would go as far as to say between 79-83 is optimal. The reason for lower temps is to keep bactirea away, they thrive at higher temps. For spores I would recommend a lower temp 79f or below but if you have a good Iso higher temps are great. CH
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JMath
Stranger

Registered: 06/06/09
Posts: 120
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Re: How do these look? (first grow) [Re: Psuper]
#10618050 - 07/03/09 09:25 PM (15 years, 6 months ago) |
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So basically the consensus is that 70-75 is fine. While moving towards 80 will give you slightly faster growth, it isn't worth the risk of contams. Correct me if I'm wrong. Either way, I have spotted growth in two more jars!
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: How do these look? (first grow) [Re: Psuper]
#10618158 - 07/03/09 09:55 PM (15 years, 6 months ago) |
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We did a comparison many years ago disproving Mr. Stamets findings. We found 81F to be the fastest growth with cubensis. I believe Roadkill was the one that was spot on. We must all remember these are core temps not incubator temps understand mycelium generates heat through metabolic activity this can range from a couple degrees in half pint jars to 10F or more in bulk subs such as dung. This means with optimal core temps of 81F mean ambient air temps in the range of 71F to 79F depending on the substrate/tek used. RR is and has been correct but has not apparently explained enough to his following the reasoning behind it. I've personally have been growing since Oss and Oeric days I bought the book when it first came out in "75" I believe. I grew more exotic molds during those first two years in search of the elusive cubie! My first success was when I gave up on a beautiful case run it wouldn't fruit for nothing I mean I waited a whole 5 days maybe 7 so I tossed it in the corner of my basement two weeks later I when to throw it out and I had shooms growing I was tripping moments later!!! I finally set up a very nice lab with an airlock chamber entrance and all the bells and whistles I could buy ($4000 worth) which was considerable overkill but I perfected my sterile culture technique and have been growing ever since not just mushrooms but spiritually as well.
Just one last thing that's been misunderstood when ever you place a casing layer on a substrate you are casing it that's why we call it a casing technically it's a cased substrate but calling it a casing is quicker to type and we all know we are then talking about a cased substrate correct no but it's myco lingo in my book. You'll see me always refer to it as a cased substrate because I respect RR and everything he does for us although we don't always agree he always has my utmost respect and I'm right there reading his replies to try to pick up on new info. Now when I say 80F is not too high it isn't too high it is on the high side but will work almost perfect I know Ive done it more times than I can remember! What we need here is more open minds not sheep we need our members to really understand the whys before the hows because the whys will answer so many of the hows before even being asked. Understanding is the key to success.
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: How do these look? (first grow) [Re: JMath]
#10618197 - 07/03/09 10:05 PM (15 years, 6 months ago) |
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Quote:
JMath said: So basically the consensus is that 70-75 is fine. While moving towards 80 will give you slightly faster growth, it isn't worth the risk of contams. Correct me if I'm wrong. Either way, I have spotted growth in two more jars!
If you've sterilized your jars correctly there is little to no risk of contamination. The point being are you in a hurry I hope not because growing mushrooms is all about patience but if you you want to take advantage of the temperature drop in combination with full colonization, a drop in co2 and the introduction if light then colonizing at slightly warmer temps can have an advantage. Understand all substrains are different my recommendations are general they will provide increased success overall CONSISTENTLY If you isolate a prolific pinner (another handle of mine at another site LOL) you don't need to do much to have consistent high yields that's why we isolate to find the best characteristics such as size, quantity, disease resistance and of course potency.
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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CH HELL
Brain Sturgeon



Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: How do these look? (first grow) [Re: hyphae]
#10618202 - 07/03/09 10:06 PM (15 years, 6 months ago) |
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Quote:
hyphae said: What we need here is more open minds not sheep we need our members to really understand the whys before the hows because the whys will answer so many of the hows before even being asked. Understanding is the key to success.
Yeap, and I am not a yes man. lol
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AlexP



Registered: 06/04/09
Posts: 2,270
Loc: Europe
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Re: How do these look? (first grow) [Re: hyphae]
#10618210 - 07/03/09 10:10 PM (15 years, 6 months ago) |
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Hyphae, if I hadn't rated you I would.
-------------------- "To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi
"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley
"The Universe is an artistic catharsis." Artifex Infinitum
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jerrylove
little bit further then I was



Registered: 06/19/09
Posts: 616
Loc: shakedown street
Last seen: 2 days, 15 hours
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Re: How do these look? (first grow) [Re: AlexP]
#10618700 - 07/04/09 12:39 AM (15 years, 6 months ago) |
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Hyphea Howdy Bro Ham. I think even with all the temp and grow speed bla bla bla stuff. The one thing no one is telling the new guy is that patients is the key. I remember my first go I would practically watch the jars all day. IT'S MADDENING I TELL YOU MADDENING!!!! lol. You need to walk away for a few days and then peek. I bet things will look better then, and tape those holes up with micro pore tape aka cloth tape.
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THOSE WHO KNOW DONT TALK! THOSE WHO DONT KNOW CANT TALK. (Timothy Leary)
"Funny thing about weekends when your unemployed they don't mean quit so much except you get hang out with your workin' friends" Les Claypool
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: How do these look? (first grow) [Re: jerrylove]
#10619916 - 07/04/09 09:37 AM (15 years, 6 months ago) |
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Quote:
jerrylove said: Hyphea Howdy Bro Ham. I think even with all the temp and grow speed bla bla bla stuff. The one thing no one is telling the new guy is that patients is the key. I remember my first go I would practically watch the jars all day. IT'S MADDENING I TELL YOU MADDENING!!!! lol. You need to walk away for a few days and then peek. I bet things will look better then, and tape those holes up with micro pore tape aka cloth tape.
Quote:
hyphae said: The point being are you in a hurry I hope not because growing mushrooms is all about patience
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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