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Offlinehooterjh101
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Registered: 06/22/09
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Last seen: 2 months, 28 days
Making clone from Colonized pf jar
    #10616953 - 07/03/09 04:51 PM (15 years, 6 months ago)

Hi all ive currently only a few jars that are in the later stages of colinization and im rather anxious to make use more of not all my production space. My question is:

Could i take a sample from a colonized pf jar, which looks like it can be done from reading many tek's on the site, And transfer it into another pre-sterilized pf jar. If so, what do you think would be the best means of doing so meaning break the myc down into small peices and inject with a syringe, place the mycelium directly into the substrate, or another method of doing the same thing?

All would be performed in a flow hood but im still interested in what other believe the safest means of doing this would be. Any opinions welcomed!


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OfflineLostkeys
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Registered: 06/06/09
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Re: Making clone from Colonized pf jar [Re: hooterjh101]
    #10616975 - 07/03/09 04:55 PM (15 years, 6 months ago)

Yes, your idea would work, but if you have access to a flow hood, why not do some agar work?  I know that's what I would be doing if I had one.


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Offlinehooterjh101
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Registered: 06/22/09
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Last seen: 2 months, 28 days
Re: Making clone from Colonized pf jar [Re: Lostkeys]
    #10617300 - 07/03/09 06:19 PM (15 years, 6 months ago)

Thanks for the quick replies, I have heard bad things about doing the agar tek with petri dishes although im quite sure my steralitity precautions would be good enough to do a LC  it just seems unlogical to  produce a seperate LC when you can use a simple method like the one mentioned above.

What would you suggest using for a sterile liquid to submerge the myc in? Distilled water, bottled water, boiling your own ect.

thanks


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OfflineLostkeys
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Re: Making clone from Colonized pf jar [Re: hooterjh101]
    #10617330 - 07/03/09 06:26 PM (15 years, 6 months ago)

Well, I've used this tek for my most recent batch of LC.  I'm using multispore, but it was designed for use in cloning.  I really like it actually, and will probably make all my LCs in this manner.


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