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stveo77
novice


Registered: 01/04/09
Posts: 17
Last seen: 15 years, 6 months
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always getting contamed???
#10615500 - 07/03/09 12:18 PM (15 years, 6 months ago) |
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i spray the room dwn with lysol. wipe wipe ,everything..and i dnt open door till done.. im still getting pretty much all contamed with green mold..i dnt understand, i sprayed the whole room and air. any suugestions
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kaneabel
getting my feet wet


Registered: 01/10/05
Posts: 133
Loc: Central Indiana
Last seen: 15 years, 1 month
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Re: always getting contamed??? [Re: stveo77]
#10615517 - 07/03/09 12:23 PM (15 years, 6 months ago) |
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look at other prep methods, how clean are you when you prepare your syringe, jars, etc?
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: always getting contamed??? [Re: stveo77]
#10615552 - 07/03/09 12:32 PM (15 years, 6 months ago) |
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Learn sterile culture technique. It isn't at all as easy as you thing you have to be methodical. You have a glovebox? a simple cardboard box with a sheet of plastic over the front works better than nothing sometimes. Usually one overlooks something so obvious. Every surface must be wiped down properly from containers to instruments to the surface your working on. If you set something down it needs to be wiped again before implementing.
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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electrics
wave rider



Registered: 12/02/08
Posts: 1,609
Loc: In front of my Computer
Last seen: 5 months, 17 days
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Re: always getting contamed??? [Re: kaneabel]
#10615558 - 07/03/09 12:34 PM (15 years, 6 months ago) |
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Yes somehow you're missing something either a PC or use new substrate material..A good glovebox is a must also reliable spores If you don't have a good print to work from that could be you're problem....Contams are really easily avoided if you folow tek's to the T hope this helps good luck in the future.........e
-------------------- "Listen now I'm talking I've been here for weeks, waiting in this growing crowd staring at my feet, The world around me is Turning I'm just standing still, The time has come for changes do something or I will" Phish
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: always getting contamed??? [Re: electrics]
#10615918 - 07/03/09 01:44 PM (15 years, 6 months ago) |
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Quote:
electrics said: Yes somehow you're missing something either a PC or use new substrate material..A good glovebox is a must also reliable spores If you don't have a good print to work from that could be you're problem....Contams are really easily avoided if you folow tek's to the T hope this helps good luck in the future.........e
Good spores! 
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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psily


Registered: 02/11/09
Posts: 116
Last seen: 13 years, 8 months
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Re: always getting contamed??? [Re: hyphae]
#10617554 - 07/03/09 07:31 PM (15 years, 6 months ago) |
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Maybe you don't sterilize your jars long enough?
I ask, because my last 3 jars got contammed super-green, and the only difference with the rest was that I PC'ed those 3 jars last (it's a small PC and I had to do 3 batches that day). Those contams were due to insufficient PC time.
-------------------- All mushrooms are edible but some are edible only once.
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BUDDHA_702
Master Mycologist In Training



Registered: 02/17/07
Posts: 1,296
Loc: Some Country
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Re: always getting contamed??? [Re: psily]
#10617571 - 07/03/09 07:35 PM (15 years, 6 months ago) |
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PF tek? do you use a glove box?
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 9 hours, 40 minutes
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Re: always getting contamed??? [Re: BUDDHA_702]
#10617588 - 07/03/09 07:39 PM (15 years, 6 months ago) |
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do u flame yur needle
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MushroomMaster
Horticulturalist



Registered: 07/02/09
Posts: 59
Loc: Deep Sea Lair
Last seen: 15 years, 6 months
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Re: always getting con tamed??? [Re: tripdawg420]
#10618020 - 07/03/09 09:16 PM (15 years, 6 months ago) |
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Make yourself a homemade glove box using a clear rubber tote.
Spray non scented Lysol into the glove box and let settle with tools and jars.
Put on some glove and flame the needle red hot and your golden.
I haven't contamed in a long time.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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What kind of jars are you shooting up? How are you prepping them? A glovebox is nice, but it's not totally necessary. But good sterile materials are.
-------------------- You make it all possible. Doesn't it feel good?
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MushroomMaster
Horticulturalist



Registered: 07/02/09
Posts: 59
Loc: Deep Sea Lair
Last seen: 15 years, 6 months
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Re: always getting con tamed??? [Re: Doc_T]
#10618039 - 07/03/09 09:24 PM (15 years, 6 months ago) |
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Sorry man but most of us would agree that its totally necessary, It's not a smart move to work with out it. Its cheap, you can buy one for around 6$. Pretty cheap. A flow hood would be great but more expensive.
If your not using a pressure cooker then I would recomend saving your money. 60$ Walmart. Pressure cookers do 90% of the sterilizing. The other 10% is all you.
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JMath
Stranger

Registered: 06/06/09
Posts: 120
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I'm only on my first grow but I feel like you don't absolutely need to use a glovebox. The alternative to using one would be doing the inoculation in the cleanest room of your house (doesn't matter where, but kitchens and bathrooms are bad). Turn off the air conditioning/heating system, shut the door, clean all of your utensils, have everything ready to go, then just don't move for a good five minutes or so to let the air settle.
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AlexP



Registered: 06/04/09
Posts: 2,270
Loc: Europe
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Re: always getting con tamed??? [Re: JMath]
#10618170 - 07/03/09 09:59 PM (15 years, 6 months ago) |
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Quote:
I'm only on my first grow but I feel like you don't absolutely need to use a glovebox.
If you're just on your first grow don't just come up with theories that go against people with thousends of grows. What do you know about it? A bathroom is the best place to do it if you clean it up well. There's hardly any wood and there are no carpets or if there are you can take them out. I spray everything with bleach, wipe everything with alcohol, use a glovebox, shower, brush teet, wear gloves, scrubs, head net...
Edit: I just wish I had a flow hood...
-------------------- "To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi
"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley
"The Universe is an artistic catharsis." Artifex Infinitum
Edited by AlexP (07/03/09 10:00 PM)
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proudabu
creeper



Registered: 12/05/08
Posts: 616
Loc: Under My Bed
Last seen: 9 years, 6 months
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Re: always getting con tamed??? [Re: JMath]
#10618178 - 07/03/09 10:01 PM (15 years, 6 months ago) |
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i personally use a small bathroom. l close the toilet seat. whipe counter with lysol cover vents and spray lysol all into the room everywhere then shut it let it sit and come back in. never had a problem with contams i also use a pressure cooker though.
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MushroomMaster
Horticulturalist



Registered: 07/02/09
Posts: 59
Loc: Deep Sea Lair
Last seen: 15 years, 6 months
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Re: always getting con tamed??? [Re: JMath]
#10618181 - 07/03/09 10:01 PM (15 years, 6 months ago) |
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You have to understand that their are all kinds of micro organisms floating all around, even walking stirs them all up. You may get lucky but why risk it? You spent your cash to get this far whats 6 more?
Just make it simple
Lysol the air and let it settle.
Cut two holes and wear gloves.
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tazz
the spazz



Registered: 04/21/09
Posts: 162
Loc: The Great White North
Last seen: 13 years, 7 months
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Re: always getting con tamed??? [Re: JMath]
#10618183 - 07/03/09 10:02 PM (15 years, 6 months ago) |
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Quote:
JMath said: I'm only on my first grow but I feel like you don't absolutely need to use a glovebox.
You don't absolutely need to, but why wouldn't you? They're dead easy to make and can be made for ultra cheap. So why not do it and help eliminate potential problems?
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 7 months, 4 days
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Re: always getting con tamed??? [Re: JMath]
#10618185 - 07/03/09 10:02 PM (15 years, 6 months ago) |
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Quote:
JMath said: I'm only on my first grow but I feel like you don't absolutely need to use a glovebox. The alternative to using one would be doing the inoculation in the cleanest room of your house (doesn't matter where, but kitchens and bathrooms are bad). Turn off the air conditioning/heating system, shut the door, clean all of your utensils, have everything ready to go, then just don't move for a good five minutes or so to let the air settle.
Not to be mean but for a person on their first grow, I wouldn't be giving that advice... Quote:
you don't absolutely need to use a glovebox
. On the other hand, you did offer some great advice.... Quote:
Turn off the air conditioning/heating system,
. That is something important that most people miss. If the OP has had zero success, then you shouldn't cut ANY corners. A glove box is simple to make, inexpensive and absolutely indispensable tool for a beginning mycologist.
--------------------
AMU Q&A thread.
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CH HELL
Brain Sturgeon



Registered: 10/02/08
Posts: 6,610
Loc: mars
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If you are using your bathroom then move to your dining room. To many germs in the bathroom. Some people get away with using the bathroom but I wouldn't risk it. GL, CH
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AlexP



Registered: 06/04/09
Posts: 2,270
Loc: Europe
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Re: always getting con tamed??? [Re: CH HELL]
#10618256 - 07/03/09 10:26 PM (15 years, 6 months ago) |
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I use my bedroom, love the smell of bleach!
-------------------- "To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi
"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley
"The Universe is an artistic catharsis." Artifex Infinitum
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Quote:
MushroomMaster said: You have to understand that their are all kinds of micro organisms floating all around, even walking stirs them all up. You may get lucky but why risk it? You spent your cash to get this far whats 6 more?
Just make it simple
Lysol the air and let it settle.
Cut two holes and wear gloves.

Perfect!
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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