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Offlinerizingfire
Mycoticus psychoticus
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Registered: 09/10/07
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Re: Jars not colonising :( [Re: Leadwing]
    #10619167 - 07/04/09 04:47 AM (15 years, 6 months ago)

Ya you can use the syringe again just flame the tip or clean with alcohol....don't re-inoculate them...just be patient...


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aka NHMI

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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: Jars not colonising :( [Re: Leadwing]
    #10619607 - 07/04/09 07:53 AM (15 years, 6 months ago)

Yes, the spore syringe can be kept at room temperature.


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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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Offlinehyphae
born to grow
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Re: Jars not colonising :( [Re: Leadwing]
    #10619834 - 07/04/09 09:01 AM (15 years, 6 months ago)

Quote:

Leadwing said:
Also, I put the syringe back in its plastic bag, in a tube, and in a room temp drawer, that's ok yesh?



That will be just fine.


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
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Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineLeadwing
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Re: Jars not colonising :( [Re: hyphae]
    #10621369 - 07/04/09 02:45 PM (15 years, 6 months ago)

Update:

ALL FOUR have signs of growth, yay! :omgawesome:


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Grab every second given to you and make love to it.

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InvisibleAlexP
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Re: Jars not colonising :( [Re: Leadwing]
    #10621402 - 07/04/09 02:55 PM (15 years, 6 months ago)

:gethigh:

Now wait till full colonization and THEN wait ANOTHER week.
After that you may dunk and roll.
GL

:flyhigh:


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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OfflineLeadwing
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Re: Jars not colonising :( [Re: AlexP]
    #10621475 - 07/04/09 03:16 PM (15 years, 6 months ago)

Do I have to dunk and roll?
I watched the video and it looks well brutal!!
Does it not increase risk of contam?


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Grab every second given to you and make love to it.

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InvisibleAlexP
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Posts: 2,270
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Re: Jars not colonising :( [Re: Leadwing]
    #10621483 - 07/04/09 03:19 PM (15 years, 6 months ago)

No way. It's great that you do it. Cakes are very resistant to contams after full colonization. You watched the videos? That's great! Any question you might have just shoot!


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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Offlinehammy
Me, Myself and I
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Re: Jars not colonising :( [Re: AlexP]
    #10621580 - 07/04/09 03:48 PM (15 years, 6 months ago)

Definitely dunk and roll it is not brutal, far from it. if you wanted more water wouldn't you want some one to give you a drink? Glad you watched the videos though


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Back after an unwanted hiatus

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Offlinehooterjh101
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Registered: 06/22/09
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Re: Jars not colonising :( [Re: Leadwing]
    #10621676 - 07/04/09 04:21 PM (15 years, 6 months ago)

Certainly sounds like what happened to me with air exchange. Eventually i lossened up the tin foil lids a bit an viola! fully colonized in 3 weeks waiting for pins.


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OfflineLeadwing
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Re: Jars not colonising :( [Re: hooterjh101]
    #10621733 - 07/04/09 04:40 PM (15 years, 6 months ago)

What's better, dunking and rolling, or casing?


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Grab every second given to you and make love to it.

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OfflineoTEMPESTo
Just let it ride


Registered: 07/04/09
Posts: 13
Loc: MICHIGAN
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Re: Jars not colonising :( [Re: Leadwing]
    #10621745 - 07/04/09 04:43 PM (15 years, 6 months ago)

Come on man. look up the info for yourself and decide. I wouldnt say this for everything but with these very beginer question's please see the search bar!

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InvisibleAlexP
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Posts: 2,270
Loc: Europe
Re: Jars not colonising :( [Re: oTEMPESTo]
    #10621768 - 07/04/09 04:53 PM (15 years, 6 months ago)

Quote:

What's better, dunking and rolling, or casing?




You'll have bigger yields when spawning to bulk and applying a casing layer but I'd dunk and roll if I was you. Do the previous for your next grow.


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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Offlineblazeup420
?got issues?

Registered: 04/30/09
Posts: 133
Last seen: 14 years, 5 months
Re: Jars not colonising :( [Re: AlexP]
    #10630066 - 07/06/09 12:58 PM (15 years, 6 months ago)

i followed the tek exactly and watched rrs vids and same problem...got new spores and tried the old formula so maybe that will work


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