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OfflineLeadwing
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Registered: 08/19/08
Posts: 208
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Jars not colonising :(
    #10610438 - 07/02/09 03:22 PM (15 years, 6 months ago)

I inoculated my jars about a week ago now, and still don't see anything!

I sterilised them, did everything properly, have kept them in a plastic container at the right temp...

Wahhh :'(

What could the reason be?


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OfflineLostkeys
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Re: Jars not colonising :( [Re: Leadwing]
    #10610450 - 07/02/09 03:25 PM (15 years, 6 months ago)

More information is required.  What type of jars?  Are your inoculation holes covered? What temperature are you colonizing at?


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OfflineDevii
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Re: Jars not colonising :( [Re: Leadwing]
    #10610455 - 07/02/09 03:25 PM (15 years, 6 months ago)

Where did you get your spores from? They might just be bunk spores...

But more likely, did you allow your jars to cool for 24 hours after you PC'd them? If you inoculate a hot jar, the temperature will kill your spores.


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OfflinePsuper
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Re: Jars not colonising :( [Re: Leadwing]
    #10610462 - 07/02/09 03:26 PM (15 years, 6 months ago)

Grains or cakes? What kind of filter? What temps? How many jars? Is their excessive moisture?

You don't need them in a plastic tub.  Can you open a jar and smell it?  A sour or old sock smell is a sign of bacteria.  No signs of growth in 7days means something is wrong. ~Pixie~


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OfflineLeadwing
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Re: Jars not colonising :( [Re: Leadwing]
    #10610760 - 07/02/09 04:31 PM (15 years, 6 months ago)

4 jars

2 part Verm, 1 part BRF, 1 part water

Around 26C


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OfflineLeadwing
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Re: Jars not colonising :( [Re: Leadwing]
    #10610771 - 07/02/09 04:33 PM (15 years, 6 months ago)

I covered the jars (each with a hole in) with 2-3 layers of foil, secured it tightly.

I didn't cover up the inoculation holes after I inoculated them, as it had dry Verm ontop.


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InvisibleAlexP
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Registered: 06/04/09
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Re: Jars not colonising :( [Re: Leadwing]
    #10610812 - 07/02/09 04:40 PM (15 years, 6 months ago)

Quote:

I covered the jars (each with a hole in) with 2-3 layers of foil, secured it tightly.





There's no gas exchange that way. Dry verm on top of the inoc holes? Never heard of anything like that...


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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Offlineblazeup420
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Registered: 04/30/09
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Re: Jars not colonising :( [Re: Leadwing]
    #10610816 - 07/02/09 04:41 PM (15 years, 6 months ago)

ive had the same problem twice still cant figure it out...is your BRF organic?mines not but that shouldnt matter...but ya i need to figure this out to...


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OfflineLeadwing
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Re: Jars not colonising :( [Re: AlexP]
    #10610823 - 07/02/09 04:42 PM (15 years, 6 months ago)

No, I mean the jars are 3/4 full of mix, and the last 1/4 is dry Verm.


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OfflineLeadwing
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Re: Jars not colonising :( [Re: Leadwing]
    #10610833 - 07/02/09 04:43 PM (15 years, 6 months ago)

Also... gas exchange?
Surely they need to be pretty much air tight so theres no chance of contams?


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InvisibleAlexP
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Re: Jars not colonising :( [Re: Leadwing]
    #10610854 - 07/02/09 04:47 PM (15 years, 6 months ago)

Quote:

Surely they need to be pretty much air tight so theres no chance of contams?


  :facepalm:

Air tight jars will stall. Haven't you watched the videos? What are you doing?... What are you following?... Remove that foil and what type of filter?... Who do the lids look like? The verm layer is there to stop contams from attacking your mycelium or substrate.


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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Offlinekaneabel
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Re: Jars not colonising :( [Re: Leadwing]
    #10610860 - 07/02/09 04:48 PM (15 years, 6 months ago)

The verm acts as a filter for the air, it should exchange air ok, sometimes near the end of colonization if it seems like it is stalling, you can turn them upside down and what happens is when you do that the mass of everything comes down and creates a vacuum effect and brings more oxygen inside the jar to finish up.

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OfflineLeadwing
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Re: Jars not colonising :( [Re: Leadwing]
    #10610888 - 07/02/09 04:53 PM (15 years, 6 months ago)

Guide

I must admit, I sort of rushed things...
I still have half a syringe left, might have another go.

Also, is it normal that the syringe had clumps in it? It looked clear apart from the clumps. (I shook it up as much as poss)

The jars I used have screw on tops


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OfflineDWreck
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Registered: 03/03/09
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Re: Jars not colonising :( [Re: Leadwing]
    #10610897 - 07/02/09 04:55 PM (15 years, 6 months ago)

well first thing take that foil off..its only used to keep out the water while p/cing. how many holes in your lid? how deep is your dry verm layer? But honestly, It sounds like you need to go watch rr's videos.

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InvisibleAlexP
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Posts: 2,270
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Re: Jars not colonising :( [Re: kaneabel]
    #10610901 - 07/02/09 04:56 PM (15 years, 6 months ago)

Quote:

you can turn them upside down and what happens is when you do that the mass of everything comes down and creates a vacuum effect and brings more oxygen inside the jar to finish up.


:facepalm:



"Don't flip jars. As prisoner said, it screws up the vermiculite filter. CO2 doesn't need to 'drain' out by flipping the jar upside down. That's a bit of disinformation that once typed refuses to die. The reason is the heat produced by the mycelium causes circulation that takes care of the gas exchange." RR


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: Jars not colonising :( [Re: AlexP]
    #10610913 - 07/02/09 04:58 PM (15 years, 6 months ago)



--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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OfflineLeadwing
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Registered: 08/19/08
Posts: 208
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Re: Jars not colonising :( [Re: Leadwing]
    #10610916 - 07/02/09 04:58 PM (15 years, 6 months ago)

Just the one (tiny) hole... I'll go take the foil off


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InvisibleAlexP
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Posts: 2,270
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Re: Jars not colonising :( [Re: Leadwing]
    #10610929 - 07/02/09 05:00 PM (15 years, 6 months ago)

And loosen up the lids. :thumbup:

Edit: And watch the videos!!! Watch a professional do the work and then follow!


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

Edited by AlexP (07/02/09 05:01 PM)

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OfflineDWreck
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Re: Jars not colonising :( [Re: Leadwing]
    #10610952 - 07/02/09 05:04 PM (15 years, 6 months ago)

There's supposed to be 4 holes. You definitely need to check out the link Alex posted. if you follow that to the t You will succeed

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OfflineLeadwing
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Registered: 08/19/08
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Re: Jars not colonising :( [Re: Leadwing]
    #10610966 - 07/02/09 05:06 PM (15 years, 6 months ago)

Oh holy crap wicked, I think there's some signs of life at the bottom of 2 of the jars! WOOOOOT

Also will the same syringe be good to use again? Its like 2/3 full


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Shop: Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate


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