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darkestmage
Medical Student / Ski Bum

Registered: 06/18/09
Posts: 195
Last seen: 11 years, 4 months
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Oven bag with WBS log - PICS
#10605753 - 07/01/09 08:04 PM (15 years, 6 months ago) |
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First I put all my WBS into a bowl. Ran hot water over it and let it sit for 5 min:
 Then dumped it into a strainer and ran the strainer through the tap:
 Put it back into the bowl and let it sit in water again. Then strained again. I did this 3 times (until the water ran clear.) Then I dumped it all into a big pot and put it on the stove to simmer for 30 min (until I noticed the first grains cracking open):
 I then let it dry out for an hour like this:
 I then rinsed everything 2 or 3 more times. Then I put everything into two oven bags. $3 for a pack of 5 at the grocery door. I double bagged them. I then used a zip tie to not seal it, but leave a small hole for gas exchange, maybe 1/4 inch hole:
 I then wrapped them in foil, lined the bottom of my PC with jar lid rings, and PC'd them at 15psi for about 75 minutes. I let the thing cool down for a couple hours. I then sprayed lysol everywhere in the room and took the bags out of the PC, immediately tightening the bags zip ties. I wore disposable gloves rinsed with alcohol when I did this. I then placed another zip tie on to keep it all tight.
For the filter I took a pin, which was soaking in alcohol, wiped down 2 areas on each bag, and popped 10 holes in each small area. So 20 holes per bag. I then quickly covered the spots in 2 layers of athletic tape:
 Everything looks like it held up during PC, no leaks in the bags or anything. I'm going to inoculate these in a couple days with PE from Sporeworks. I might chicken out at the last minute and just use an ecuador print on these test bags and use my PE on some jars that I know will work fine...We'll see. I'm really hoping nothing goes wrong because I've been dieing to grow PE and don't want to waste my only syringe. I might shoot 5 CC's in one bag and the other 5 in PFtek jars to be safe.
Thanks
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kaneabel
getting my feet wet


Registered: 01/10/05
Posts: 133
Loc: Central Indiana
Last seen: 15 years, 1 month
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Re: Oven bag with WBS log - PICS [Re: darkestmage]
#10605844 - 07/01/09 08:17 PM (15 years, 6 months ago) |
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sweet...
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darkestmage
Medical Student / Ski Bum

Registered: 06/18/09
Posts: 195
Last seen: 11 years, 4 months
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Re: Oven bag with WBS log - PICS [Re: kaneabel]
#10605979 - 07/01/09 08:38 PM (15 years, 6 months ago) |
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You guys think the double layered athletic tape with pin holes under it will be sufficient GE? I know they're sterile...the GE issue is the only thing keeping me from injecting expensive spores.
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mushshoo
Stranger

Registered: 07/26/08
Posts: 60
Last seen: 15 years, 2 months
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Re: Oven bag with WBS log - PICS [Re: darkestmage]
#10606323 - 07/01/09 09:40 PM (15 years, 6 months ago) |
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looks pretty good
-------------------- Looking for plant tissue cultures pm me
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Stealthgrower
n00b Pwner



Registered: 04/19/09
Posts: 622
Last seen: 6 months, 7 days
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Re: Oven bag with WBS log - PICS [Re: mushshoo]
#10606683 - 07/01/09 10:38 PM (15 years, 6 months ago) |
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I don't think this is going to work man and I promise I'm not trying to burst your bubble. Go with the ecuador and just test it I guess, but I'd start over. I think it was ohmatic who posted a sweet oven bag tek. May have been Wronguy. I tried to search it for you but no luck. http://www.shroomery.org/forums/showflat.php/Number/562556 Here's a slight variation, I recommend securing a layer or so of tyvek instead of polyfill but either will work. Good luck. Stay sterile!
-------------------- Infea said:
You ever seen that movie Constantine. Well they pass into hell several times. In hell there are melting human bodies, in your tub are melting mushroom bodies. Your tub had passed into mushroom hell.
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