Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Kratom Powder For Sale   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineCarcass
Polarized
Male User Gallery

Registered: 02/15/09
Posts: 407
Last seen: 8 years, 17 days
Potato Dextrose Agar
    #10591909 - 06/29/09 11:32 AM (15 years, 6 months ago)

Hi guys.
I'm coming up to a new level: entering the world of culture.
I have 200 grams of PDA, the right water volume for it, is 500 ml, isn't it?

How long should i sterilize it?
Is there any other information that i should know?

Thanks!

Edited by Carcass (06/29/09 11:37 AM)

Extras: Filter Print Post Top
InvisibleDr Qi
Old Hippie & Marry Prankster!
 User Gallery


Registered: 09/04/07
Posts: 15
Loc: Divine state of Ubiquity ...
Re: Potato Dextrose Agar [Re: Carcass]
    #10592316 - 06/29/09 12:57 PM (15 years, 6 months ago)

Hi: It's 40 grams per liter of DI water, use 20 grams into 500ml of DI water that's being heat on a boiling water bath (just a pan of boiling water to put the bottle your using to mix the agar into to heat it) for just long enough for the PDA powder to completely go into solution and then sterilize at 250f (that's 15 psi in your PC) for 30 minutes. I use a 750 ml liqueur bottles that looks like a florentine flask or you could use those 750 ml bottles that the premixed snaps drinks come in. Both are the right size for 500ml of media and have a long nick for easy poring of the plates. I cover the top with a piece of heavy aluminum foil that's secured with a silicone rubber band that can be found at hobby shops. Let it cool down to the point its starting to pores like a thick gravy and remember it can only be reheated for poring once. See RR video short on agar and plating if you don't have his DVD. Good Luck: Dr Qi


--------------------
In life there are few words and witticisms that I may use when writing that have not been said or written in the past. So there is little that's unique to my writings. I do borrow and use ideas and information from published authors without making any claim that its original work done by myself. Just the best information that I have available at the time when writing, as the most I can do is to search for the truth and wisdom when writing.

May you learn to know yourself and live life happy, joyous and free; Dr Q'i

Extras: Filter Print Post Top
OfflineCarcass
Polarized
Male User Gallery

Registered: 02/15/09
Posts: 407
Last seen: 8 years, 17 days
Re: Potato Dextrose Agar [Re: Dr Qi]
    #10592734 - 06/29/09 02:24 PM (15 years, 6 months ago)

Thank you very much. This information is just enough for me to start :thumbup:

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Kratom Powder For Sale   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,479 8 02/25/05 11:03 AM
by agar
* Malt and agar dosent react after 12 days !!! TheMessenger 1,254 2 05/05/03 01:02 AM
by Prisoner#1
* Agar recipe work well for germinating these five spores? KingCobWeb 10,389 8 02/24/03 11:48 PM
by Anno
* -=Newbies 411 Agar for n00bs=- ThatDaltonGuy 4,416 16 04/27/04 10:02 AM
by ThatDaltonGuy
* Agar plaastikbob 1,198 6 01/29/02 09:20 AM
by Seuss
* agar with malted barley syrup Hongosmeester 7,455 17 10/27/19 10:42 PM
by ABCMORPHS
* agar from whole foods Brady 1,055 2 11/07/04 11:13 PM
by scatmanrav
* Re: agar....allright so i'm dumb! JustAnotherTraveler 2,863 10 03/17/01 03:39 PM
by morlahere

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
395 topic views. 25 members, 87 guests and 35 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.007 seconds on 12 queries.