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OfflineRoseM
Devil's Advocate
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Registered: 09/24/03
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Trusted Cultivator
Re: One monotub two strains? [Re: RogerRabbit]
    #10589549 - 06/28/09 10:59 PM (15 years, 6 months ago)

Quote:

RogerRabbit said:
Quote:

Cervantes said:
They DO compete or people wouldn't isolate strains. :wink:





Not exactly, and that isn't why we isolate strains.

The same thing happens every time you use multispore inoculation, which could just as accurately be called multi-strain inoculation.  They don't compete any more than the thousands of hyphae that emerge from an inoculation site.  I've done this hundreds of times. 

Try it different ways just for grins and giggles.  Inoculate two holes of a jar with one strain and the other two holes with another.  Or, inoculate each of the 4 holes with a separate syringe(strain), for four strains per jar.  You can mix them at spawning or mix them at inoculation.  Do a few each way.  I doubt you'll be able to tell any difference between them all, at least no more than you'd normally see in a multispore grow.
RR




Why would people isolate strains if they weren't worried that a lesser, competing strain would eventually dominate (or compete) for their grow?

You've entirely missed my point. Perhaps I was unclear.

I never said anything to suggest that combining different 'Strains' would be very different from using any other multispore/bulk method. But I'm not gonna pretend that using myc from two different varieties is the most efficient way to grow a cube either. AND to combine them this late in the game, when fruiting is imminent? It is a waste of energy. Perhaps it is irrelevant in your eyes, but it is energy that could be put immediately into colonization and fruiting.

I'd be interested in seeing the differences between the two 'Strains' he bought. Perhaps one is more prolific than the other...

You'd rather play Mad Scientist with his grow. Nothing wrong with that. 'Strains' bore you.

I completely agree with what you've said. And yet, I still stand by my previous statements.


--------------------
Fiddlesticks.


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InvisibleDoc_T
Random Dude
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Registered: 03/06/09
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Re: One monotub two strains? [Re: Rose]
    #10591264 - 06/29/09 07:45 AM (15 years, 6 months ago)

Quote:

Cervantes said:
I completely agree with what you've said. And yet, I still stand by my previous statements.




There should be a Blue Ribbon for posts like that.


--------------------
You make it all possible. Doesn't it feel good?

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Offlinekinderfeld11
kill your master


Registered: 12/13/08
Posts: 315
Last seen: 15 years, 5 months
Re: One monotub two strains? [Re: Doc_T]
    #10591880 - 06/29/09 11:25 AM (15 years, 6 months ago)

Yea for real two trusted cultivators with different point of views. I am almost more confused than when I posted this LOL.

That is a super sweet pic of PE/EQ!

So cervantes you CAN agree that mixing strains will reach the same potential as just innocing with any one strains?

This subject still seems to be quite contradictory...as I found in my searching as well.

The last thing I want is energy/yield not being used to full potential if that is the case.

I think dividing the tub is best options as of now. From all that I have read I am still iffy on this whole mixing strains thing.


But to be clear RR you are saying that there will be absolutley no difference as far as potential growth/yield (other than what the different strains allow)by innoc with a one multispore strain compared to multiple multispores strains?

Quote:

Cervantes said:
I never said anything to suggest that combining different 'Strains' would be very different from using any other multispore/bulk method.

But I'm not gonna pretend that using myc from two different varieties is the most efficient way to grow a cube either.




I am just confused by this so using more than one strain is as good as using any multispore but it is not the most efficient way to grow a cube, if it is the same or there is not difference how can one be more or less efficient than the other? Or are you talking about mixing innoc in that first sentance and mixing spawn in the second?

So your ok with mixing innoc, but not spawn?


EDIT: Ok yea I am still REALLY confused...


--------------------
Cowards die many times before their deaths;
The valiant never taste of death but once.
Of all the wonders that I yet have heard.
It seems to me most strange that men should fear;
Seeing that death, a necessary end,
Will come when it will come.
(Julius Caesar, Act II, Scene 2).
:mushroom2:Host of new 'Kinderfeld's Kindness' monthly contest.:mushroom2:

Edited by kinderfeld11 (06/29/09 11:39 AM)

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OfflineRoseM
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Re: One monotub two strains? [Re: kinderfeld11]
    #10592116 - 06/29/09 12:18 PM (15 years, 6 months ago)

Just 'cause we have trusted cultivator tags doesn't mean we're experts in all things. I did most of my cultivation research over 5 years ago, and things change quickly in this hobby. When in doubt, listen to RR.

If RR says it won't make a difference, he's probably right... or close enough to being correct that you'll hardly notice a difference. It really comes down to what YOU want to get out of your grow, 'cause either way, you should get plenty of shrooms.

By the time you're spawning your tub, It does seem a little late in the game to intentionally mix two different 'Strains', chances are slim that the two 'strains' would cross with one another, since all the viable spores have already mated... so both 'Strains' will be competing for the tub's substrate, and nobody knows exactly what you'll end up with. Neither RR nor I knows for sure exactly what will happen. Neither of us has played with your  jars. We just like giving advice...

If you knocked up any jar with the spores of two different 'strain' syringes (like RR suggested), it'd be no different than multispore innoc from one syringe... EXCEPT you wouldn't know exactly what kind of shrooms you'd get. One 'Strain' may dominate the other... OR there is a chance you'd get a PE/EQ like combo... which is pretty fucking cool, but hardly a guarantee.

But if you have never grown either 'strain' and you think they'll both look similar (PE looks unique so it is easier to tell when it crosses with something else) how the hell will you know what you ended up with?

If you wish to cross 'Strains' there are better, more accurate and more specific ways to do it... so you can know for sure that you actually crossed them.

I see no reason to mix them unless you want to experiment. If you DO want to experiment, it would probably be fun to try... and you'll still get shrooms assuming you set your tub up properly.

By keeping them separate, it will be easier to know which is which, and if you wish to make and trade prints, it will be easier to know exactly what you will be trading. It is much easier to trade a Texas print than it is to trade a print that might be Texas or it might be Koh Samui.

Also, if one of your spawn jars is less productive, or slower than the other... you may get better results from BOTH if you keep them separate... because they'll both have a chance to fruit at their own pace.

So, if you are interested in seeing BOTH 'Strains', keep 'em separate. If you are interested in mixing and matching, try that. It'd be a fun experiment.

Usually people asking questions in the Cultivation Forum want to avoid taking chances. Mixing two different 'strains' is taking an extra chance... because, aside from mushrooms, you won't exactly know what you got. I believe RR is correct, however, when he suggests it will have little effect on your ultimate bulk.

I hope that helps.


--------------------
Fiddlesticks.


Edited by Rose (06/29/09 01:15 PM)

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Offlinekinderfeld11
kill your master


Registered: 12/13/08
Posts: 315
Last seen: 15 years, 5 months
Re: One monotub two strains? [Re: Rose]
    #10593746 - 06/29/09 06:11 PM (15 years, 6 months ago)

Excellent post. Absolutely EXCELLENT.

Very interesting not many people experiment with this, I guess it's because like you said, there are better ways to cross strains. However If the only 'chance' taken being taken is not knowing the kind of mushroom, seems like a small chance to me.

I still don't see why I don't see many 'split' tubs. Why would people not more variety of mushrooms and prints? If the potential yield is just the same...

Thanks for everyone's help I will be dividing them, and I will post pics so you guys can see. I am just a new grower though I don't eat shrooms (for real) so I wouldn't be able to tell you any of that kind of info...

This got me to thinking maybe a very large 5 or 10 strain (or mixed strain(s) :smile:) monotub will be a future project :laugh: 10 different strain spore prints from 1 grow sounds good to me.

Wish me luck.

Edited by kinderfeld11 (06/29/09 06:18 PM)

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