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kinderfeld11
kill your master


Registered: 12/13/08
Posts: 315
Last seen: 14 years, 6 months
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One monotub two strains?
#10588022 - 06/28/09 06:42 PM (14 years, 7 months ago) |
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Ok so I want to innoc like 16 jars but I have no two same strain syringes but have a couple of different variety. I also want a decent amount of innoc for faster performance. I have searched and find people say it could cause problems (mutants), some say it's fine, some say they will fight, some say you'll get crosses, some say you get a little of both or you might just get one.
My main question is can I mix 8 jars of spawn of one strain to one side of the monotub and 8 jars of another strain to the other half... if that would be suffice to grow two strains in the same tub and if they would fruit at full potential. Any problems doing this?
I read that mixing them via inoculation is a toss up but I didn't find much if I just mixed up the crumbled cakes....of both strains...would this be a problem? Is the above idea better for using more than on strain or should I mix cakes or innoc?
I searched but this is a very confusion subject to research. I have not found anything to suggest separating the spawn to the bulk wouldn't work so any advice mucho appreciated.
-------------------- Cowards die many times before their deaths; The valiant never taste of death but once. Of all the wonders that I yet have heard. It seems to me most strange that men should fear; Seeing that death, a necessary end, Will come when it will come. (Julius Caesar, Act II, Scene 2).
Host of new 'Kinderfeld's Kindness' monthly contest.
Edited by kinderfeld11 (06/28/09 06:45 PM)
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Yeah, do it half and half. Put a piece of cardboard in to help set it up, then pull that out carefully.
-------------------- You make it all possible. Doesn't it feel good?
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kinderfeld11
kill your master


Registered: 12/13/08
Posts: 315
Last seen: 14 years, 6 months
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Re: One monotub two strains? [Re: Doc_T]
#10588125 - 06/28/09 07:02 PM (14 years, 7 months ago) |
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Ok sweet so by help "set up" do you mean I pull out the cardboard after colonization of both sides or do I take it out after each side is all mixed up?
Sorry for the bombardment of Qs...
-------------------- Cowards die many times before their deaths; The valiant never taste of death but once. Of all the wonders that I yet have heard. It seems to me most strange that men should fear; Seeing that death, a necessary end, Will come when it will come. (Julius Caesar, Act II, Scene 2).
Host of new 'Kinderfeld's Kindness' monthly contest.
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OogieBoogie
Stranger



Registered: 05/20/09
Posts: 961
Loc: Shadow On The Moon
Last seen: 9 years, 4 months
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I've seen a few things about this splitting the tub half and half  definitely keep us updated id like to see !
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electrics
wave rider



Registered: 12/02/08
Posts: 1,609
Loc: In front of my Computer
Last seen: 4 years, 11 months
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Re: One monotub two strains? [Re: OogieBoogie]
#10588168 - 06/28/09 07:11 PM (14 years, 7 months ago) |
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What ever happened to a cube is a cube!! I've never had mycelium battles or only one strain win...I've currently mixed equador with CRS on accident of course but the result has been 2 flushes of very nice mushrooms just my experience.......e
-------------------- "Listen now I'm talking I've been here for weeks, waiting in this growing crowd staring at my feet, The world around me is Turning I'm just standing still, The time has come for changes do something or I will" Phish
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 10 years, 4 months
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Re: One monotub two strains? [Re: OogieBoogie]
#10588183 - 06/28/09 07:13 PM (14 years, 7 months ago) |
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sounds like a lot of spawn for a monotub.
Should work well either way on the divider.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?" "The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything." - Author and former M6/M5 agent John le Carré on Democracy Now. Conquer's Club
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 1 month
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I think ive actually done this with 1 of my mini monos. I crumbled 3 cakes into it.
And then a few days later i was going to do another and noticed that i was missing 1 jar of 3 of the same strain which isnt in any of my 2 litre soda bottles, so we will see how it goes.
I seem to remember RR syaing that different strains have a 1 in 4 chance of joining.
the certainly dont battle or compete.
If your using spores, dont use more solution, you dont really get faster colinisation. What growth you initially see faster is all the spores germinateing and its ust a larger mass of mycelium, it still has to join together with the other networks which wastes energy.
Ive not noticed much difference between jars that accidentaly got too much solution compared with those that got just a 1/4 CC per hole in pf cakes. The cake that got too much showed alot more mycelium to begin with, but colinised in roughly the same time as the other jars.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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fungusfeller
TIMMY



Registered: 05/14/09
Posts: 549
Loc: South Park
Last seen: 12 years, 5 months
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Re: One monotub two strains? [Re: electrics]
#10588241 - 06/28/09 07:26 PM (14 years, 7 months ago) |
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noc up all the same, are you concerned you don't have enough of one syringe to do 16 jars? if so just put a jar of sterile water in your glove box with your jars. when your syringe gets low draw up some water and continue, and repeat if necessary. when jars are all noced up fill your syringe up for next time.
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TIMMY PUKESPLASH
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Rose
Devil's Advocate



Registered: 09/24/03
Posts: 22,518
Loc: Mod not God
Last seen: 1 year, 6 months
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Re: One monotub two strains? [Re: electrics]
#10588293 - 06/28/09 07:38 PM (14 years, 7 months ago) |
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Quote:
electrics said: What ever happened to a cube is a cube!! I've never had mycelium battles or only one strain win...I've currently mixed equador with CRS on accident of course but the result has been 2 flushes of very nice mushrooms just my experience.......e
Chances are BOTH types of cube would struggle for superiority and this energy could be better spent if it were directed into fruiting. True, the results could be negligible.
However...
Dividing the two would also, make it easier to know which was which.
-------------------- Fiddlesticks.
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 10 years, 4 months
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Re: One monotub two strains? [Re: Rose]
#10588753 - 06/28/09 09:03 PM (14 years, 7 months ago) |
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I remember RR said they don't compete. Cube is a cube, they won't fight and if this dude wants to do half and half I don't think it'll be a problem at all. Kinda might be interesting to see them both preform in identical conditions.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?" "The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything." - Author and former M6/M5 agent John le Carré on Democracy Now. Conquer's Club
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Rose
Devil's Advocate



Registered: 09/24/03
Posts: 22,518
Loc: Mod not God
Last seen: 1 year, 6 months
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They DO compete or people wouldn't isolate strains. 
But either way, the results will usually produce shrooms.
-------------------- Fiddlesticks.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: One monotub two strains? [Re: Rose]
#10588780 - 06/28/09 09:08 PM (14 years, 7 months ago) |
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You can grow two strains side by side in a tub. I've done it. It worked fine. Both sides fruited just like I was expecting them to.
-------------------- You make it all possible. Doesn't it feel good?
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Lucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
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Re: One monotub two strains? [Re: Doc_T]
#10588826 - 06/28/09 09:19 PM (14 years, 7 months ago) |
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Quote:
They DO compete or people wouldn't isolate strains.
They'll compete for nutrients and water, but they won't kill one another off. They will both live and fruit if they are both viable fruiting strains and are both given proper conditions.
Like Doc said, they can both prosper and fruit together. Or another possibility is the two separate dikaryotic networks can fuse together and exchange genetic sets. I forget the scientific name for this, I remember it from one of Stamets books.
-------------------- PAN CYAN & AZURE FOR TRADE "If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha "Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-
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Mnboardin
Stranger


Registered: 04/01/09
Posts: 767
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Let them combine and see what comes out of it, and if you get something a little different mail me a spore print, or a piece of tissue. 
Check out my Penis envy/Ecuadorian mix-

--------------------
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
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Re: One monotub two strains? [Re: Rose]
#10589179 - 06/28/09 10:14 PM (14 years, 7 months ago) |
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Quote:
Cervantes said: They DO compete or people wouldn't isolate strains. 
Not exactly, and that isn't why we isolate strains.
The same thing happens every time you use multispore inoculation, which could just as accurately be called multi-strain inoculation. They don't compete any more than the thousands of hyphae that emerge from an inoculation site. I've done this hundreds of times.
Try it different ways just for grins and giggles. Inoculate two holes of a jar with one strain and the other two holes with another. Or, inoculate each of the 4 holes with a separate syringe(strain), for four strains per jar. You can mix them at spawning or mix them at inoculation. Do a few each way. I doubt you'll be able to tell any difference between them all, at least no more than you'd normally see in a multispore grow. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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iluvfungi


Registered: 06/17/09
Posts: 1,488
Loc: Oakland, CA USA
Last seen: 12 years, 9 months
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Re: One monotub two strains? [Re: RogerRabbit]
#10589239 - 06/28/09 10:21 PM (14 years, 7 months ago) |
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It gets somewhat technical but this is what happens when both strains grow in the same substraight; it generally creates a new strain. I can't explain it at a genetic detail, but the same is true if you pick a wild fungi and it is growing next to something poisonous; the result is a poisonous fungi.
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 10 years, 4 months
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Re: One monotub two strains? [Re: Rose]
#10589257 - 06/28/09 10:24 PM (14 years, 7 months ago) |
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Quote:
Cervantes said: They DO compete or people wouldn't isolate strains. 
Actually Mr. TC we isolate so that we can crank out consistently potent/prolific/large fruits as these traits tend to be genetic most of the time. This is why we don't always keep some isolates we make.
They will as RR had said not fight against each other just consume the nutrients separately. You will not loose in yields in any noticeable way. Especially if you are doing them the way the OP said by dividing them half and half.
edit: wow slow reply on my part
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?" "The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything." - Author and former M6/M5 agent John le Carré on Democracy Now. Conquer's Club
Edited by ScavengerType (06/28/09 10:26 PM)
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
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Re: One monotub two strains? [Re: iluvfungi]
#10589313 - 06/28/09 10:31 PM (14 years, 7 months ago) |
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Quote:
iluvfungi said: but the same is true if you pick a wild fungi and it is growing next to something poisonous; the result is a poisonous fungi.
That my friend is flat-out wrong. I can't count the number of P cyanescens I've picked that were right next to and sometimes touching deadly galerinas. I've picked edibles in the forest that were inches away from death caps. They don't transfer from one to another. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Lucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
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Re: One monotub two strains? [Re: RogerRabbit]
#10589491 - 06/28/09 10:51 PM (14 years, 7 months ago) |
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Misinformation sucks
-------------------- PAN CYAN & AZURE FOR TRADE "If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha "Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 1 month
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Re: One monotub two strains? [Re: Mnboardin]
#10589531 - 06/28/09 10:56 PM (14 years, 7 months ago) |
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Quote:
Mnboardin said: Let them combine and see what comes out of it, and if you get something a little different mail me a spore print, or a piece of tissue. 
Check out my Penis envy/Ecuadorian mix-


Thats awesome!
I have 1 jar doing exactly what RR said, inoculate with 2 different sttrains, i have 8 inoc holes, 2 grouped together with 2 different strains, i need to birth that jar, the damn things pinning.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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