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OfflineJohnP
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Xtremely weak mushrooms?
    #10588761 - 06/28/09 09:05 PM (15 years, 6 months ago)

So a friend of mine just had his first flush of brazillian cubensis. 5.5 grams dried was consumed over an hour and all that happened was a slight body buzz and no visuals. They were cracker dry. They were grown using brf cakes with verm from an oooold liquid culture that had been in the fridge for at least 4 or 5 months before innocuoating, this is what I suspect would be the cause? Besides that, light was on and off random times whenever he felt like switching it on or off. Lights remained on more time than not but never a consistent schedule. Not much misting, once pins appeared he stopped misting and heavily fanned anywhere from 2 to 8 times per day.

He is now on his second flush, are they too bound to be impotent like the first flush? Should he scrap the project and start a new from fresh lc? And what would be the easiest most accessable way to step up yeild and still remain stealth? Any words on sclerotia?  Thanks!

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Invisiblecurbstop
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Registered: 06/02/09
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Re: Xtremely weak mushrooms? [Re: JohnP]
    #10588791 - 06/28/09 09:10 PM (15 years, 6 months ago)

Was the lc made with MS


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OfflineJohnP
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Re: Xtremely weak mushrooms? [Re: curbstop]
    #10588825 - 06/28/09 09:18 PM (15 years, 6 months ago)

I am unsure. I am not familiar with the differences between multispore and whatever else... I figure they all contain thousands of spores? By ms do you mean multiple strains in one? And why do that if so? If it helps answer your question, my friend got his syringe from ralph, brazillian. Whatever that would come as.

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Offlineveda_sticks
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Re: Xtremely weak mushrooms? [Re: JohnP]
    #10588854 - 06/28/09 09:23 PM (15 years, 6 months ago)

thats what can happen when growing from MS, ie a spore syringe which will contain hundreds of thousands of spores. potency is ginetic so you probably got ginetics that produced weak mushrooms.

Im sure an LC stored in the fridge for 4-5 months is fine.


--------------------

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Invisiblecurbstop
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Registered: 06/02/09
Posts: 810
Re: Xtremely weak mushrooms? [Re: JohnP]
    #10588862 - 06/28/09 09:24 PM (15 years, 6 months ago)

MS=Multispore - Refers to an inoculation where multiple germinations and matings occur due to the use of various spores, as in a spore solution (e.g. spore syringe) and as opposed to an isolate. Liquid

The reason i ask is
Quote:

are they too bound to be impotent like the first flush? Should he scrap the project and start a new from fresh lc?


My answer is
1. no ms can give you good and poor potency on anygive flush from any given mushroom on the same flush.
2.No read one again, You could get dynamight potency no matter what flush it is with MS
3.It's never a bad idea to make new lc but if the old is still good why bother. It will not effect potency.


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InvisibleLucid_Euphoria
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Re: Xtremely weak mushrooms? [Re: curbstop]
    #10588909 - 06/28/09 09:34 PM (15 years, 6 months ago)

Quote:

1. no ms can give you good and poor potency on anygive flush from any given mushroom on the same flush.




I may be wrong but I don't think that statement is true.

The issue with MS, is when a spore germinates it sends out mycelium that is monokaryotic (containing half a genetic code, most monokaryotic mycelium is not fruitable). When two monokaryotic myceliums meet, they exchange genetic information and become a single dikaryotic mycelial network. So by the time you fruit from a multispore inoculation, the fruits should all contain similar genetics, potency being one of them.

At least that is my understanding from my readings.


--------------------
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"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-

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Invisiblecurbstop
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Re: Xtremely weak mushrooms? [Re: Lucid_Euphoria]
    #10588929 - 06/28/09 09:37 PM (15 years, 6 months ago)

Quote:

I may be wrong but I don't think that statement is true.

The issue with MS, is when a spore germinates it sends out mycelium that is monokaryotic (containing half a genetic code, most monokaryotic mycelium is not fruitable). When two monokaryotic myceliums meet, they exchange genetic information and become a single dikaryotic mycelial network. So by the time you fruit from a multispore inoculation, the fruits should all contain similar genetics, potency being one of them.

At least that is my understanding from my readings.



This might be true. Im going off past practice,Meaning ive ate choomys from cake "A" and had an ok experience, I then ate choomys from Cake "A" again on a different flush and was blown away. same drop(Amount) same everything.


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Offlineveda_sticks
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Re: Xtremely weak mushrooms? [Re: Lucid_Euphoria]
    #10588965 - 06/28/09 09:44 PM (15 years, 6 months ago)

Quote:

Lucid_Euphoria said:
Quote:

1. no ms can give you good and poor potency on anygive flush from any given mushroom on the same flush.




I may be wrong but I don't think that statement is true.

The issue with MS, is when a spore germinates it sends out mycelium that is monokaryotic (containing half a genetic code, most monokaryotic mycelium is not fruitable). When two monokaryotic myceliums meet, they exchange genetic information and become a single dikaryotic mycelial network. So by the time you fruit from a multispore inoculation, the fruits should all contain similar genetics, potency being one of them.

At least that is my understanding from my readings.





Thats not quite correct. It is very possible for fruits potency to vary not only from flush to flush but even mushroom to mushroom within the same flush and that often happens.

If you have ever seen agar work with a cloned tissue, it cans ometimes infact produce sectors. And those sectors while still part of the same mycelial network are 'strains' different ginetic makeups.

when you inoculate form spores, you will have hundreds of spores and a percentage of them germinate grow monokyrotic myc and jioon with other compatible myc to form dykirotic myc, which can also join with other mycelial networks if compatible. Even though you usually have 1 complete netowrk, its made up from lots of 'strains'.

So you can see how fruits grown can have different ginetic make up and even be made up of several 'strains'

They may have similar ginetic makeups, but similar means not the same so they can be different and 1 of those traits is potency.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Invisiblegeorge castanzaM
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Re: Xtremely weak mushrooms? [Re: JohnP]
    #10589010 - 06/28/09 09:52 PM (15 years, 6 months ago)

Sounds like a triple dose is in order.


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InvisibleLucid_Euphoria
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Re: Xtremely weak mushrooms? [Re: veda_sticks]
    #10589041 - 06/28/09 09:56 PM (15 years, 6 months ago)

Quote:

It is very possible for fruits potency to vary not only from flush to flush but even mushroom to mushroom within the same flush and that often happens.




I agree with most of what you said, however, the above comment is more a subjective opinion than a scientific fact. Potency is such a relative term. Unless there are studies I haven't seen that weighed out extracted alkaloids from different mushies, etc. Which is quite possible, I just haven't seen any definitive research of it.

Quote:

If you have ever seen agar work with a cloned tissue, it cans ometimes infact produce sectors. And those sectors while still part of the same mycelial network are 'strains' different ginetic makeups.




So then do fruit bodies from a strain isolate contain 100% non sectoring mycelium? Because if not then strain isolation seems like it wouldn't produce consistant fruits.


--------------------
PAN CYAN & AZURE FOR TRADE

"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-

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Invisiblecurbstop
Regnarts


Registered: 06/02/09
Posts: 810
Re: Xtremely weak mushrooms? [Re: george castanza]
    #10589050 - 06/28/09 09:58 PM (15 years, 6 months ago)

Quote:

Sounds like a triple dose is in order.


when in doubt double it, LOL i did that the last time,Turned out to be the best dam experience to date. Man that was fun :thumbup:


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Offlineevildee125
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Registered: 03/23/09
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Re: Xtremely weak mushrooms? [Re: Lucid_Euphoria]
    #10589065 - 06/28/09 10:00 PM (15 years, 6 months ago)

yeah unfortunatley my last brazil grow was crap.. although ive gotten killers out of them too


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