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BrandNewbie
Captain



Registered: 05/21/08
Posts: 2,932
Loc: U.S.A.
Last seen: 5 years, 7 months
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Re: 3 Of 8 jars MAY BE CONTAMINATED.. very suspicious. [Re: hooterjh101]
#10586412 - 06/28/09 01:32 PM (15 years, 6 months ago) |
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Toss it.
-------------------- Question: Why do women wear make-up and perfume?
Answer: Because they're ugly and they stink.
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hooterjh101
Stranger

Registered: 06/22/09
Posts: 53
Last seen: 2 months, 28 days
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Re: 3 Of 8 jars MAY BE CONTAMINATED.. very suspicious. [Re: BrandNewbie]
#10588422 - 06/28/09 08:00 PM (15 years, 6 months ago) |
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Quote:
BrandNewbie said: Toss it.
Thats probably what ill end up having to do to the 3 jars, But im hoping i got them out of the enviroment of the healthy jars soon enough to prevent any of it from spreading to any of the non hazordous jars that seem to be colonizing great.
Also, how cruicial is frequent air exchange inside the jars during incubation?, Meaning the intake and outtake of air and c02 throughout the substrates parameters inside the colonizing jar. Because i have jars double layered with tinfoil wrapped loosely around the mouths of jars, the second layer being sealed with no innouclation holes in it,
Thinking maybe there was too much c02 buildup inside the jars and that somehow made the BRF and verm degrade creating the compost like smell? Im not experienced at all cus im fairly new to this hobby, but hoping someone from the forums can put in a 2cents of knowlege and experience i alawys get from the shroomery community.
Peace
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 5 years, 13 days
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Re: 3 Of 8 jars MAY BE CONTAMINATED.. very suspicious. [Re: hooterjh101]
#10588602 - 06/28/09 08:34 PM (15 years, 6 months ago) |
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Most certainly sounds like bacteria.
How were you sterilising your jars? what kind of flour are you using. Flame sterilsiing needle etc? glovebox?
How were you inoculating. To be honest, ive only had bacteria in 1 jar and that is surprising since i steam sterilsiing in a terrible pot that has to have a blutack seal around the lid to keep it form boiling dry.
Metabolites can be yellow, but you need mycelium to have metobolites.
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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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JewelessCaesar
Shroomery's Legal Department




Registered: 05/26/06
Posts: 599
Loc: Petros-Joyner, TN, USA
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Re: 3 Of 8 jars MAY BE CONTAMINATED.. very suspicious. [Re: hooterjh101]
#10589904 - 06/28/09 11:46 PM (15 years, 6 months ago) |
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Quote:
hooterjh101 said:
None of the jars were actually OPENED exposing the verm layer but they are only covered in 2 layers of tin foil the first having inoculation holes and the second put on fairly losely to promote a small amount of gas exchange, but this worries me that the comtams could have gotten in that way.
I dont think its mycelia piss because its growing in the jars that havent even germinated yet. So i dont think it could be a bi product of the mycelia growth for there is none except in the one jar which seems to have healthy mycelia growth.
Yeah if the same yellow is growing inside the jars that have not started to colonize, pretty safe bet that you just wanna start over. Sorry about the shit with the thread and arguing.. he seems to be banned now(johnwayne)..lol Oh well...community as a whole is better off...Well I wish you nothing but luck!..
-------------------- Failure is not an option, it's an adventure! - JewelessCaesar
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hooterjh101
Stranger

Registered: 06/22/09
Posts: 53
Last seen: 2 months, 28 days
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Re: 3 Of 8 jars MAY BE CONTAMINATED.. very suspicious. [Re: veda_sticks]
#10598192 - 06/30/09 02:47 PM (15 years, 6 months ago) |
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Quote:
veda_sticks said: Most certainly sounds like bacteria.
How were you sterilising your jars? what kind of flour are you using. Flame sterilsiing needle etc? glovebox?
How were you inoculating. To be honest, ive only had bacteria in 1 jar and that is surprising since i steam sterilsiing in a terrible pot that has to have a blutack seal around the lid to keep it form boiling dry.
Metabolites can be yellow, but you need mycelium to have metobolites.
Thought id follow up here it seems the one of the 3 with mycelium growth is stilll growing as fast as others and not smelling any worse, The other 2 are still stalled but im honestly thinking i just forgot to knock them up cus i was high on yay when i did my inoculation (oops) and double inoculated a few jars instead of the 2 at all. no harm done. Which explains why somthing else beggan to grow instead of mycelium. i threw those out.
The one jar however is about 80% and going stong so ill stilll birth it if it forms pins probably in a seperate terratium though just to be safe.
The weird thing though is that out of this batch i made a mini pf cake using a shotglass, noc'd it up and let it sit behind my computor with the exaust fan blowing all kinds of shit right on it and its stayed healthy and colonized in 5 days and is now pinning in a mini terrarium with no signs of contamination. it was really just a test to see if it would get contammed, but the jars in my HEPA air filtered incubator did instead? kinda intersting.
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