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InvisibleElSeta
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Registered: 06/26/09
Posts: 127
Loc: Atlantida.
Pleurotus Eryngii agar media color alteration to orange/red?
    #10577428 - 06/26/09 03:37 PM (14 years, 7 months ago)

I'm growing P. Eryngii on agar media of potato dextrose and yeast with H2O2. I have noticed in everyone of the cultures that in the media, (like on the back side of the petri dish) the color has turned orange or reddish-like color right under the mycelium growth. There is a picture on the shroomery website of the back side of a petri dish of P. Dimajor that somebody posted and the color is exactly as mine. I have asked many people about this color alteration and no one has seen that in their cultures. The picture I'm talking about is this https://files.shroomery.org/files/09-20/231644974-Djamor_Plate_3.jpg

the petri on the right side shows the same color as mine. I would like to know if this color is due by some sort of contamination or what? I hope you can give me a clue about this. Thanks a lot.


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Looking for Lion's Mane and Yellow Oyster culture. Will trade for: Shiitake/King Oyster/Blue Oyster/White Beech.
Me for info.



Yes, you can see the real reality through a little window. Now, build a door.


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InvisibleBlimeyGrimey
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Posts: 3,790
Loc: Puget Sound
Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ElSeta]
    #10577488 - 06/26/09 03:46 PM (14 years, 7 months ago)

Its normal for some species to discolor the agar as it grows. Its probably just the agar medium being broken down by digestive enzymes.


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Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

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OfflineScavengerType
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: BlimeyGrimey]
    #10577583 - 06/26/09 04:04 PM (14 years, 7 months ago)

I use food coloring in my agar so as to better see the mycelium and cause it's just plain cool. I also see the coloring removed by the mycelium after it has colonized the agar thoroughly. It's likely a reaction of the agar being broken down.


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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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OfflineMycelio
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ElSeta]
    #10578132 - 06/26/09 06:02 PM (14 years, 7 months ago)

The mycelium of pleurotus species secretes orange droplets when being stressed by contamination or environmental conditions.

Carsten


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InvisibleElSeta
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Registered: 06/26/09
Posts: 127
Loc: Atlantida.
Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: Mycelio]
    #10581245 - 06/27/09 11:23 AM (14 years, 7 months ago)

Could it be the Hydrogen Peroxide that caused the mycelium to react while being exposed to it and therefore make the mycelium produce some sort of enzyme that cause oxidation?


--------------------
-  -  -  -  -  -  -  -  -  -  -  -  -
Looking for Lion's Mane and Yellow Oyster culture. Will trade for: Shiitake/King Oyster/Blue Oyster/White Beech.
Me for info.



Yes, you can see the real reality through a little window. Now, build a door.


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OfflineScavengerType
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ElSeta]
    #10581551 - 06/27/09 01:02 PM (14 years, 7 months ago)

yes, that is a condition that will stress any mycelium culture.


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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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InvisibleJef
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Registered: 12/02/08
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ScavengerType]
    #10581744 - 06/27/09 01:59 PM (14 years, 7 months ago)

I grew it on H2O2 and it didn't discolor mine.  I should tell you though, some species never really grow on it.

I use it only when I want to slow growth.

Blue food colouring on no-peroxide MEY Agar.  See how the djamor has bleached the blue, and the pink of the mycelium.







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I am my own lab rat.


Tell me and I will forget. Show me and I will remember.  Involve me and I will learn.


Edited by Jef (06/27/09 06:56 PM)


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InvisibleElSeta
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Registered: 06/26/09
Posts: 127
Loc: Atlantida.
Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: Jef]
    #10583182 - 06/27/09 07:57 PM (14 years, 7 months ago)

Jeff do you have a picture of the back side of the petri dish?


--------------------
-  -  -  -  -  -  -  -  -  -  -  -  -
Looking for Lion's Mane and Yellow Oyster culture. Will trade for: Shiitake/King Oyster/Blue Oyster/White Beech.
Me for info.



Yes, you can see the real reality through a little window. Now, build a door.


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OfflineBuckeye Oysters
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ElSeta]
    #10583358 - 06/27/09 08:28 PM (14 years, 7 months ago)

Peroxide has no purpose in mycology except to stop cobweb mold.  Otherwise it damages the mycellium at the cellular level.  High incubation temps are most likely the culprit.


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Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.


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InvisibleJef
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ElSeta]
    #10583844 - 06/27/09 10:21 PM (14 years, 7 months ago)

Here you go.



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I am my own lab rat.


Tell me and I will forget. Show me and I will remember.  Involve me and I will learn.


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OfflineScavengerType
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: Jef]
    #10583887 - 06/27/09 10:29 PM (14 years, 7 months ago)

kinda looks redish to me like his, but you shouldn't drink so much coffee before taking pics.

BTW what's that red stuff where the parafilm (or substitute) on your dishes should be?


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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


Edited by ScavengerType (06/27/09 10:31 PM)


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InvisibleJef
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Registered: 12/02/08
Posts: 764
Loc: near Duncan, BC
Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ScavengerType]
    #10585564 - 06/28/09 09:42 AM (14 years, 7 months ago)

It's caffeine withdrawal and the DTs.  I'm rock steady when I've had my coffee with a double shot.:laugh:

Around the dish is some kind of stretchy rubbery tape that doesn't stick.  It's what I use instead of parafilm up here in Canada where we live in igloos and will soon have our first telephone installed, at Rick Mercer's house.  It is also very handy for hog-tying the wild nauga during the spring nauga hunts in Saskatchewan.

I have a very festive pink version too, but you'll only see that on special occasions.

Good growing,

Jef


--------------------
I am my own lab rat.


Tell me and I will forget. Show me and I will remember.  Involve me and I will learn.


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InvisibleJef
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Registered: 12/02/08
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ScavengerType]
    #10585598 - 06/28/09 09:56 AM (14 years, 7 months ago)

.


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OfflineScavengerType
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: Jef]
    #10585778 - 06/28/09 11:01 AM (14 years, 7 months ago)

Quote:

Around the dish is some kind of stretchy rubbery tape that doesn't stick.



You mean, electrical tape?

hey uhh you ever heard of the saran wrap method? Saran wrap of the LD-PE variety is oxygen permeable and cheap as fuck. I took one roll and cut it into 2" sections with a hacksaw and it worked like a charm.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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InvisibleElSeta
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Registered: 06/26/09
Posts: 127
Loc: Atlantida.
Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ScavengerType]
    #10586276 - 06/28/09 01:02 PM (14 years, 7 months ago)

Jey Jef your media is turning out reddish as mine. That's the same color I've been talking about. But is it Pink Oyster? or Eryngii?... By the way, sometimes I use pipe fitting tape on petri dishes to allow gas exchange and it's cheap too. Thaks for the pic.


--------------------
-  -  -  -  -  -  -  -  -  -  -  -  -
Looking for Lion's Mane and Yellow Oyster culture. Will trade for: Shiitake/King Oyster/Blue Oyster/White Beech.
Me for info.



Yes, you can see the real reality through a little window. Now, build a door.


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OfflineMephistophelian
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ElSeta]
    #10586490 - 06/28/09 01:48 PM (14 years, 7 months ago)

You mean like Teflon tape?


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Master Slants Made Simple


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OfflineScavengerType
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Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: Mephistophelian]
    #10586675 - 06/28/09 02:15 PM (14 years, 7 months ago)

I think he means like plumber tape used to fit threaded pipes to threaded ends and prevent liquid from seeping through the threads. An example of such a connection would be a shower fixture.

edit: this is not what jef is using though.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


Edited by ScavengerType (06/28/09 02:15 PM)


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InvisibleJef
Out-of-work Sex Slave
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Registered: 12/02/08
Posts: 764
Loc: near Duncan, BC
Re: Pleurotus Eryngii agar media color alteration to orange/red? [Re: ScavengerType]
    #10587570 - 06/28/09 05:07 PM (14 years, 7 months ago)

No. it's some kind of ribbon without adhesive but a bit clingy like saran.  I don't know if little girls use it for their pig tails, or it is for decoration somehow.

The little girl I stole it from didn't say.

Y'know money doesn't grow on trees and it's not easy to get good stuff in the middle of nowhere. I'll have to order some parafilm to be put on the next dog sled team coming out my way.

Jef


--------------------
I am my own lab rat.


Tell me and I will forget. Show me and I will remember.  Involve me and I will learn.


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