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OfflineThunderShroomer
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Registered: 05/16/09
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Good or Bad LC? Pictures included.
    #10585894 - 06/28/09 11:40 AM (14 years, 8 months ago)

Hey can anyone tell me if this is good Pan Sub growth or contam? It is a honey and water solution in a fairly sterile environment. Its hard to tell from the pictures, but it is more spider-web, stringy-like than fuzz-like.



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"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:

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InvisibleShroominit
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Re: Good or Bad LC? Pictures included. [Re: ThunderShroomer]
    #10585902 - 06/28/09 11:43 AM (14 years, 8 months ago)

Doesn't look good to me at all, still may be worth testing on a half pint PF tek jar, but it should be fluffy and almost jellyfish-like in appearance, not grainy or chunky like I think I see.

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Invisiblemisfitdeviate

Registered: 06/20/09
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Re: Good or Bad LC? Pictures included. [Re: Shroominit]
    #10585914 - 06/28/09 11:46 AM (14 years, 8 months ago)

that is fucked, should be a little cloud of white settled at the bottom

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InvisibleDoc_T
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Re: Good or Bad LC? Pictures included. [Re: misfitdeviate]
    #10585922 - 06/28/09 11:49 AM (14 years, 8 months ago)

Looks ok enough that I'd shoot a test jar. :thumbup:


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OfflineAgarAgar
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Re: Good or Bad LC? Pictures included. [Re: ThunderShroomer]
    #10585940 - 06/28/09 11:54 AM (14 years, 8 months ago)

If you have a microscope you can mount a drop on a slide and look.

AgarAgar


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Keep those mushrooms a growin

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InvisibleAlexP
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Re: Good or Bad LC? Pictures included. [Re: Doc_T]
    #10585941 - 06/28/09 11:54 AM (14 years, 8 months ago)

Looks ok to me. Test it. It was shaken, that's why it looks that way.


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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InvisibleShroominit
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Re: Good or Bad LC? Pictures included. [Re: AlexP]
    #10585981 - 06/28/09 12:03 PM (14 years, 8 months ago)

Quote:

misfitdeviate said:
that is fucked, should be a little cloud of white settled at the bottom



My mycelium often floats at the top, also that was obviously just shaken.


Quote:

AlexP said:
Looks ok to me. Test it. It was shaken, that's why it looks that way.



Looks a lot like bacteria to me, but it could just be the photograph. The only real way to tell is to test it anyway.

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Offlinehyphae
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Re: Good or Bad LC? Pictures included. [Re: Shroominit]
    #10586058 - 06/28/09 12:19 PM (14 years, 8 months ago)

FYI anything non-white is a contam in LC period. Also back lighting light makes it very difficult to tell anything try strong front lighting. Three different jars? Second pic looks great third looks very suspect and first could be a toss up. Contams often show up as simple black dots in LC and sometimes overlooked but discovered soon after knocking up a dozen jars! What a waste!!!  :frown:


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineGordy
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Re: Good or Bad LC? Pictures included. [Re: hyphae]
    #10586759 - 06/28/09 02:38 PM (14 years, 8 months ago)

Hyphae, i use malt extract to grow out my LC's and i will often get myc that has some brown stuff on it but that's just malt extract. messed me up once i thought it was just malt and i had wetspot :mad: neways, i found out after that that it will suck up all the brown and everything out of the water if you let it sit long enough in the jar, or even better suck it up into a bunch of 12cc syringes.

Thunder, like everybody else said test it out on a jar or two and see if it grows out.


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Edited by Gordy (06/28/09 02:40 PM)

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OfflineCyber
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Re: Good or Bad LC? Pictures included. [Re: hyphae]
    #10586821 - 06/28/09 02:52 PM (14 years, 8 months ago)

Quote:

hyphae said:
FYI anything non-white is a contam in LC period. (




Hyphae, that is not exactly true. LC with Malt Extract in it can have all kinds of crap that is non white and still be good. Plus if you use a dark syringe you can end up with big black clumps of spores that make it look bad. I have made LC where I was not careful and ended up with chunks of gunk in it after it was PC'd. Did not effect the growth and did not contaminate.

If those were made with Malt Extract and Dextrose, they could be fine. I would make a PF Tek Jar and test it. You can end up with stuff that you can not see that is a contaminate as well and it never hurts to test them first.

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Offlinehyphae
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Re: Good or Bad LC? Pictures included. [Re: Cyber]
    #10587032 - 06/28/09 03:35 PM (14 years, 8 months ago)

OK true true and true. I myself using karo thats all thats needed for LC and it makes spotting contams much easier. I hear ya on the staining (malt) and when using honey there can certainly oddities. As far as spores this is true they like to clump so shake shake shake. I use mag stir plates myself. I guess I'm so used to using clear LC I forgot about all the others sorry. Point taken!


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineCyber
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Re: Good or Bad LC? Pictures included. [Re: hyphae]
    #10587670 - 06/28/09 05:25 PM (14 years, 8 months ago)

I tend to use dextrose LC's for the same reason and have just gone over to using stir bars and my magnetic stirrer to mix it up. The only reason it came to mind is that I have done some testing with Malt Extract and other things and had several jars that looked like that. :lol:

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Invisiblemisfitdeviate

Registered: 06/20/09
Posts: 762
Loc: ESSR
Re: Good or Bad LC? Pictures included. [Re: Shroominit]
    #10587960 - 06/28/09 06:28 PM (14 years, 8 months ago)

Quote:

Shroominit said:
Quote:

misfitdeviate said:
that is fucked, should be a little cloud of white settled at the bottom



My mycelium often floats at the top, also that was obviously just shaken.




Its still black whether you shake it or not

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OfflineTweexican
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Re: Good or Bad LC? Pictures included. [Re: AgarAgar]
    #10588016 - 06/28/09 06:41 PM (14 years, 8 months ago)

Quote:

AgarAgar said:
If you have a microscope you can mount a drop on a slide and look.

AgarAgar




That's the best advice yet...


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Offlinehyphae
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Re: Good or Bad LC? Pictures included. [Re: Tweexican]
    #10588026 - 06/28/09 06:43 PM (14 years, 8 months ago)

Quote:

Tweexican said:
Quote:

AgarAgar said:
If you have a microscope you can mount a drop on a slide and look.

AgarAgar




That's the best advice yet...



As long as you know what your looking at! :wink:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineThunderShroomer
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Registered: 05/16/09
Posts: 44
Last seen: 5 years, 4 months
Re: Good or Bad LC? Pictures included. [Re: hyphae]
    #10588136 - 06/28/09 07:04 PM (14 years, 8 months ago)

Those pictures were all of the same jar... Just shaken and swished around. Without my help, it has all settled to the bottom. I just shook it so that the strands would be more visible. I do not see any other colors than white, but my hesitation is that it is not the cloudy white I was expecting. I have no microscope, but I will try to test it on a jar and see what happens.


--------------------
"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:

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InvisibleShroominit
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Re: Good or Bad LC? Pictures included. [Re: misfitdeviate]
    #10589468 - 06/28/09 10:47 PM (14 years, 8 months ago)

Thundershroomer, if it has almost coarse or chunky bits, that is quite characteristic of bacteria. Mycelium will always look cloudy or jellyfish-like.

Quote:

misfitdeviate said:
Quote:

Shroominit said:
Quote:

misfitdeviate said:
that is fucked, should be a little cloud of white settled at the bottom



My mycelium often floats at the top, also that was obviously just shaken.



Its still black whether you shake it or not




I like how you quoted me just to prove that you ignored my post. What I mean to say is that you would be a great politician.

My post essentially said that it doesn't have to be sunk to the bottom, especially after mixing. I didn't even mention coulor, thus I was not disagreeing with you on that point at all.

Furthermore, I would still bet that it is bacteria.


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Invisiblemisfitdeviate

Registered: 06/20/09
Posts: 762
Loc: ESSR
Re: Good or Bad LC? Pictures included. [Re: Shroominit]
    #10592481 - 06/29/09 01:36 PM (14 years, 8 months ago)

whoa, calm down buddy. I thought you were the original OP so my bad :rolleyes: And yeah, something LOOKS wrong with it. When I shake my LC it looks nothing like that and im sure its ok because I knocked up some jars and theyre contam free


After a good shake /\

Edited by misfitdeviate (06/29/09 01:42 PM)

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InvisibleShroominit
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Re: Good or Bad LC? Pictures included. [Re: misfitdeviate]
    #10595453 - 06/30/09 12:05 AM (14 years, 8 months ago)

misfit, I was and am perfectly calm, it's just annoying to be called out on something that I didn't even write.

Your LC is clear, if it was a Malt Extract LC with a bit of glass/stirrer/marble in there it gets significantly more broken up and looks darker... like in the OP, try to take a picture facing the light instead of having light behind you, gives that weird dark look, even in a clear solution. Still does not look like pictures in the OP, I will get pics of mine tomorrow.

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InvisibleAlexP
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Re: Good or Bad LC? Pictures included. [Re: Shroominit]
    #10596621 - 06/30/09 08:13 AM (14 years, 8 months ago)

The dark look is because it is honey which is not completely used by the mycelium.

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