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Ar53Lsto
Intrigued

Registered: 06/26/09
Posts: 85
Last seen: 5 years, 5 months
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Please help determining possible contamination. (PICS)
#10578070 - 06/26/09 05:49 PM (14 years, 7 months ago) |
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This was my first attempt using the PF Tek. I inoculated 6 jars with P. cubensis B+ and followed Tek as best I could. I have read a little about different types of contamination, but I am not sure if my project is totally lost. As you can see in the pics, there are "wet spots" around the jar, but there is no discoloration of the mycelium at all. I would consider it to be healthy other than these spots. The mycelium growth rate has however been what I would call, very slow! The jar in this pic has taken almost a month to reach this mycelium level, and it is the best of the lot. The other jars have about half the amount of growth as this one. As far as smell goes, there is no foul odor at all. If this is indeed bacteria, are my cakes lost completely or can they be saved? Thanks!



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bumpn
jesus juice


Registered: 05/31/09
Posts: 948
Loc: HELL
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Re: Please help determining possible contamination. (PICS) [Re: Ar53Lsto]
#10578215 - 06/26/09 06:15 PM (14 years, 7 months ago) |
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looks wet do u have the lid rubber side up if u dont losten it a little to dry it out a little no expert so dont quote me on this
THE CANNING JAR LID (loose or tight) There are two choices with the lids during incubation - tight or loose. With a very high moisture content (good for fruiting), a tight lid can cause water to collect in the bottom of the jar. This is to be avoided. If it happens, the lid should be kept on loose during incubation. Tape the canning jar lid to the band to make the lid act as a one piece lid for raising and lowering. If the substrate is on the dry side, a tight lid will preserve the moisture content. It is all a matter of the balance between the water needs of the mycelium, the size of the jar, the available air space in the jar and the type of vermiculite used. Only by simple experimenting and comparison can the right balance be found for a given set of conditions. Take notes and go with what fruits the best.
After the substrate turns white with the mycelium (2 or 3 weeks after inoculation), the jars are left to sit in indirect light. The mycelium will continue to infiltrate the substrate until it gets enough food to trigger the fruiting cycle. In less than a week to a few weeks after surface colonization of the cake, tiny white "pin" like structures begin to appear. This is called pinning. This is the beginning of the fruiting cycle. Soon after that, within the week, small round fungus growths appear that soon begin to turn yellow.
i just knocked up 12 jars myself they look to be on the dry side. rubber side up. and i started to see Mycelium after only 3 days
-------------------- [if u have PB print PM ME] {-----------------------------------------------------------------------------------------------------------------}
 
Edited by bumpn (06/26/09 06:19 PM)
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Ar53Lsto
Intrigued

Registered: 06/26/09
Posts: 85
Last seen: 5 years, 5 months
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Re: Please help determining possible contamination. (PICS) [Re: bumpn]
#10578238 - 06/26/09 06:19 PM (14 years, 7 months ago) |
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I have the lids rubber side up and they are all on loose to the point where you could just lift them off without screwing.
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bumpn
jesus juice


Registered: 05/31/09
Posts: 948
Loc: HELL
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Re: Please help determining possible contamination. (PICS) [Re: Ar53Lsto]
#10578251 - 06/26/09 06:21 PM (14 years, 7 months ago) |
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well then ull need to wait for an expert to comment. sry i couldn't help
-------------------- [if u have PB print PM ME] {-----------------------------------------------------------------------------------------------------------------}
 
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bumpn
jesus juice


Registered: 05/31/09
Posts: 948
Loc: HELL
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Re: Please help determining possible contamination. (PICS) [Re: bumpn]
#10578273 - 06/26/09 06:26 PM (14 years, 7 months ago) |
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i used the search function and seen some talk about fliping the jars to let the verm asorb it, and they say to watch out for pooling witch can cause rot but they say a little water is ok
ps im soo jealous i hate waiting this is all i got so far
-------------------- [if u have PB print PM ME] {-----------------------------------------------------------------------------------------------------------------}
 
Edited by bumpn (06/26/09 06:32 PM)
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mycolusician
Stranger



Registered: 05/27/09
Posts: 36
Last seen: 14 years, 5 days
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Re: Please help determining possible contamination. (PICS) [Re: Ar53Lsto]
#10579650 - 06/26/09 11:11 PM (14 years, 7 months ago) |
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youre fine
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Ar53Lsto
Intrigued

Registered: 06/26/09
Posts: 85
Last seen: 5 years, 5 months
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Re: Please help determining possible contamination. (PICS) [Re: mycolusician]
#10584536 - 06/28/09 12:50 AM (14 years, 7 months ago) |
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Thanks...I appreciate the replies!
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Othyem



Registered: 04/17/09
Posts: 1,935
Last seen: 3 years, 4 months
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Re: Please help determining possible contamination. (PICS) [Re: Ar53Lsto]
#10584579 - 06/28/09 01:01 AM (14 years, 7 months ago) |
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They look good to me. Good luck fruiting.
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