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Lucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
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Culture Timeline
#10584077 - 06/27/09 11:12 PM (15 years, 6 months ago) |
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So I'm trying to plan out the best procedures to take to get the most out of a strain isolate. I haven't isolated yet, but I figured I should have a game plan for once I have.
So this is what I have come up with:
1. Strain Isolation
2. Inoculation of Master Slants (Long Term Low Temp Storage)
3. Transfer from Slant to a single grain jar (this step is to ensure the strain is still vigorous as well as free of contamination)
4. Once grain jar is nearing 100% colonization, use Agar's Grain LC Tek to create a "Master" Non Nutritious LC (NNLC)
5. The Master LC is then used to inoculate nutritious LC's (Light Dried Malt Extract)
6. Nutritious LC then used to inoculate grain spawn
7. If extra spawn is required, G2G transfers to multiply spawn
8. Fruit
All comments are welcome. Do I have too many steps? Would you change anything?
-------------------- PAN CYAN & AZURE FOR TRADE
"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha
"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-
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AgarAgar
Mushroom Cultivator
Registered: 06/23/09
Posts: 70
Last seen: 15 years, 10 days
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Looks good to me.
AgarAgar
-------------------- Keep those mushrooms a growin
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Lucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
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Re: Culture Timeline [Re: AgarAgar]
#10587831 - 06/28/09 05:55 PM (15 years, 6 months ago) |
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Anymore opinions?
-------------------- PAN CYAN & AZURE FOR TRADE
"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha
"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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You thinkQuote:
Lucid_Euphoria said: Anymore opinions?
You think too much.
-------------------- You make it all possible. Doesn't it feel good?
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Psuper
Psilocybin

Registered: 02/12/08
Posts: 2,878
Loc: PNW
Last seen: 1 month, 1 day
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You can get a lot of that grain LC from a single jar. I wouldn't bother with the additional nutritive LC unless you really need that much inoculant. Just keep a master slant and do more grain LC or g2g when you need.
That's just me though, maybe you would benefit from having a jar of LC on hand. ~Pixie~
-------------------- Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk
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Psuper
Psilocybin

Registered: 02/12/08
Posts: 2,878
Loc: PNW
Last seen: 1 month, 1 day
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Re: Culture Timeline [Re: Psuper]
#10587874 - 06/28/09 06:07 PM (15 years, 6 months ago) |
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Everything else sounds spot-on. Best of luck. ~Pixie~
-------------------- Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk
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Lucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
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Re: Culture Timeline [Re: Psuper]
#10588097 - 06/28/09 06:56 PM (15 years, 6 months ago) |
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Quote:
You think too much.
Libra/Scorpio Cusp man...
its in the stars they tell me.
-------------------- PAN CYAN & AZURE FOR TRADE
"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha
"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-
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