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InvisibleLucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
Bulk Tub Contammed, Help with procedure please
    #10575890 - 06/26/09 09:53 AM (15 years, 6 months ago)

I just a monotub contam on me. I'm not sure the cause of my contams but my educated guess would be:
a) The bulk sub wasn't pasteurized properly
or
b) A contam got in during the inoculation with spawn

Here is my rundown:
I followed this tek by Large_dose for bulk pasteurization.

When I went to spawn to the bulk, I had to adjust moisture content on the bulk sub, so added some water and mixed it in. Of course this is all done in open air, which I'm guessing is where my contam came into play. I stood outside of a small closet that I use as a work area. Then I poured the sub into a monotub, took out my spawn, scooped the spawn into the sub using a spoon cleaned with alcohol. Mixed the spawn into the sub, leveled it out, & placed some wax paper down onto  it. Then covered it up, put it in a black trash bag, threw it in a closet with a blanket over it and let it go for 2 weeks. Temp inside the closet was prolly in the low to mid 80's. Went to check it today and saw what I think was trich on one side of the sub, and another contam in a different area which looked a bit different to me than the other.

So I'd like to know what other steps people take during spawn inoculation to ensure against contam particles floating into your sub? I turned shut the air vents in the room to prevent drafts and such. I'm going to spawn another today and want to try to take more preventive measurements against this happening again.


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PAN CYAN & AZURE FOR TRADE

"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-

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OfflineCyber
Ash
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Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 1 year, 8 months
Re: Bulk Tub Contammed, Help with procedure please [Re: Lucid_Euphoria]
    #10575928 - 06/26/09 10:01 AM (15 years, 6 months ago)

You could clean the room and close / tape off the vents, then put a hepa filter in the room and run it for a couple of hours. After that you could spray the room down with lysol.

You could also up the spawn ratio so that the contam does not have time to get hold.

Truth is, you are the third person in 2 days that I have heard complain of contam and was using that tek.

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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: Bulk Tub Contammed, Help with procedure please [Re: Lucid_Euphoria]
    #10575931 - 06/26/09 10:02 AM (15 years, 6 months ago)

I can't say plainly enough how much I hate the process of using a spoon to get spawn out of a jar.  The spoon cuts many of the grains in half, leaving the uncolonized center of the grains exposed to mold spores. 

Instead, I suggest banging the jar against a tire, phone book, or block of wood to break up the individual grains, and then simply pour them out.

Bulk substrates need not be sterile, nor do you need to spawn in sterile conditions.  I doubt that was the problem.  Either your spawn was contaminated from the get-go, or you damaged the grains when you spooned them out and that's where the contamination got its foothold.

I'm sure others will come along and say they've used a spoon before with no problems, but I stand by the above.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleLucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
Re: Bulk Tub Contammed, Help with procedure please [Re: RogerRabbit]
    #10575952 - 06/26/09 10:08 AM (15 years, 6 months ago)

Thanks guys. I will be sure not to use a spoon anymore, and follow your directions RR. I'm also going to go find a cheapo hepa air cleaner to run in the room, cause I need one anyway :grin:

So as far as adjusting moisture content on the substrate in open air, you don't think that is a big deal? Should be safe enough?


--------------------
PAN CYAN & AZURE FOR TRADE

"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-

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InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Bulk Tub Contammed, Help with procedure please [Re: RogerRabbit]
    #10575961 - 06/26/09 10:09 AM (15 years, 6 months ago)

:sad: I've always used a spoon... :blush:

What about a rubber spatula?
I don't have room for a tire in my apartment, and my hand gets sore breaking up fully colonized quarts. What other ways are there to break up grain spawn?


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You make it all possible. Doesn't it feel good?

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InvisibleLucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
Re: Bulk Tub Contammed, Help with procedure please [Re: Doc_T]
    #10575990 - 06/26/09 10:18 AM (15 years, 6 months ago)

I'm gonna try the phonebook. I'm just afraid I'm gonna break the jar.


--------------------
PAN CYAN & AZURE FOR TRADE

"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-

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OfflineEdgeChaos
Still a stranger


Registered: 08/04/06
Posts: 2,071
Last seen: 7 years, 5 months
Re: Bulk Tub Contammed, Help with procedure please [Re: Doc_T]
    #10575993 - 06/26/09 10:18 AM (15 years, 6 months ago)

I have always just bashed the jar on some rolled up carpet. You could use a mouse pad.

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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: Bulk Tub Contammed, Help with procedure please [Re: Doc_T]
    #10575994 - 06/26/09 10:19 AM (15 years, 6 months ago)

Quote:

I don't have room for a tire in my apartment




Go to your local bicycle shop and buy or get for free a wrecked bike wheel that the tire will still hold air.  Pump it up to 60 psi and use that.  Works like a charm, as shown in my DVD.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleAlexP
 User Gallery


Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: Bulk Tub Contammed, Help with procedure please [Re: RogerRabbit]
    #10576208 - 06/26/09 11:19 AM (15 years, 6 months ago)

HAHAHAHAHAH RR, I LOVE your signature!


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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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InvisibleLucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
Re: Bulk Tub Contammed, Help with procedure please [Re: AlexP]
    #10576272 - 06/26/09 11:33 AM (15 years, 6 months ago)

Ok I spawned another tub. The phonebook worked great btw RR. Unfortunately I forgot to reserve some substrate for a top layer so there are some exposed grains, hopefully this won't be an issue. Also, how necessary is it to use foil/wax on top of the sub to help along colonization? Does it just help speed it along or is it a significant difference over not using any?


--------------------
PAN CYAN & AZURE FOR TRADE

"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-

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OfflineOutThisLife
Male


Registered: 03/27/09
Posts: 1,296
Loc: 127.0.0.1
Last seen: 4 years, 10 months
Re: Bulk Tub Contammed, Help with procedure please [Re: Lucid_Euphoria]
    #10576407 - 06/26/09 11:59 AM (15 years, 6 months ago)

Lol I'm using that tek and it's going perfectly.

When did you notice contams?

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Offlinebrand0n
large jar mad man!!


Registered: 02/09/09
Posts: 842
Last seen: 10 years, 6 months
Re: Bulk Tub Contammed, Help with procedure please [Re: OutThisLife]
    #10576416 - 06/26/09 12:02 PM (15 years, 6 months ago)

i smack jars against my hand and can get them to break up.

rr thanks on the spoon info, ill be sure to keep that in mind from here out.

makes sense.


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That did it! I am now the anti BRF CAKE! I have had enough! TO HELL WITH BRF! REAL MEN USE SEED! Go now brf younging. Get some seed. COME BACK A MAN!

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InvisibleLucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
Re: Bulk Tub Contammed, Help with procedure please [Re: OutThisLife]
    #10576417 - 06/26/09 12:02 PM (15 years, 6 months ago)

Quote:

Lol I'm using that tek and it's going perfectly.



As stated above, I don't think the tek was the problem.

Quote:

When did you notice contams?



I only checked the tub once, 13 days after I spawned it.


--------------------
PAN CYAN & AZURE FOR TRADE

"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-

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OfflineOutThisLife
Male


Registered: 03/27/09
Posts: 1,296
Loc: 127.0.0.1
Last seen: 4 years, 10 months
Re: Bulk Tub Contammed, Help with procedure please [Re: OutThisLife]
    #10576437 - 06/26/09 12:06 PM (15 years, 6 months ago)

Hmm.. Right now my tub is looking great and smells wonderful. I'm guessing you had that too?

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InvisibleLucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
Re: Bulk Tub Contammed, Help with procedure please [Re: OutThisLife]
    #10577617 - 06/26/09 04:10 PM (15 years, 6 months ago)

Quote:

Right now my tub is looking great and smells wonderful. I'm guessing you had that too?




Are you just rambling or are you being a wise ass? I can't tell.

Yes I've had success using these procedures.


--------------------
PAN CYAN & AZURE FOR TRADE

"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha

"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-

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OfflineOutThisLife
Male


Registered: 03/27/09
Posts: 1,296
Loc: 127.0.0.1
Last seen: 4 years, 10 months
Re: Bulk Tub Contammed, Help with procedure please [Re: Lucid_Euphoria]
    #10587250 - 06/28/09 04:14 PM (15 years, 6 months ago)

Quote:

Lucid_Euphoria said:
Quote:

Right now my tub is looking great and smells wonderful. I'm guessing you had that too?




Are you just rambling or are you being a wise ass? I can't tell.

Yes I've had success using these procedures.




Both.

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