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OfflineKagenical
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Registered: 05/27/08
Posts: 128
Last seen: 11 years, 2 months
Storing Sterile grain jars?
    #10570544 - 06/25/09 12:30 PM (15 years, 6 months ago)

I don't currently own a PC...

However, I have the chance to borrow a 32qt All American PC from work which is absolutely fantastic. I get it for the entire weekend as well.

I'm thinking about sterilizing some WBS jars for a good 90 minutes @ 15 psi (allowing for the PC to come up to pressure, of course) and storing them in the fridge afterwards for a few weeks.

This way, I can use the last of my syringes in a few wbs jars, then do a G2G transfer at a later date (when the first jars colonize) to knock up the rest of em.

How does this sound to everyone? I could technically freeze the jars as well (but I worry it would burst some of the seed).

Opinions wanted!

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InvisibleDoc_T
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Storing Sterile grain jars? [Re: Kagenical]
    #10570629 - 06/25/09 12:45 PM (15 years, 6 months ago)

"a few weeks" is kinda long, you'll have a higher contam rate than usual.


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You make it all possible. Doesn't it feel good?

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OfflineKagenical
Stranger

Registered: 05/27/08
Posts: 128
Last seen: 11 years, 2 months
Re: Storing Sterile grain jars? [Re: Doc_T]
    #10570739 - 06/25/09 01:06 PM (15 years, 6 months ago)

Figured as much. I'd only be waiting as long as it takes for the first jar to colonize before I do a G2G.

In reality, I doubt they'd be sitting for more than 15 days or so, as brazillian myc tears through WBS IME.

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InvisibleCH HELL
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Registered: 10/02/08
Posts: 6,610
Loc: mars Flag
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Re: Storing Sterile grain jars? [Re: Kagenical]
    #10570919 - 06/25/09 01:35 PM (15 years, 6 months ago)

Can't you borrow it again in a couple weeks?  The grain jars are only partially sterilized and after two weeks the endo spores begin to multiply. 
GL,
CH

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OfflineStealthgrower
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Registered: 04/19/09
Posts: 622
Last seen: 6 months, 7 days
Re: Storing Sterile grain jars? [Re: Doc_T]
    #10570947 - 06/25/09 01:39 PM (15 years, 6 months ago)

Quote:

Doc_T said:
"a few weeks" is kinda long, you'll have a higher contam rate than usual.



:thumbup:
My 16 qt presto is the best investment I've ever made into this hobby.


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Infea said:
You ever seen that movie Constantine.  Well they pass into hell several times.  In hell there are melting human bodies, in your tub are melting mushroom bodies.  Your tub had passed into mushroom hell.

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