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Offlinextofury
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Registered: 12/18/08
Posts: 588
Last seen: 14 years, 11 months
Re: How necessary is Lysol? [Re: fungusfeller]
    #10571979 - 06/25/09 04:13 PM (15 years, 6 months ago)

eew who the heck lives in a place with carpet?  Most places use laminates, ceramic or stone tiles or hardwood.
Do it in a room that is one of those.

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Offlinextofury
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Re: How necessary is Lysol? [Re: xtofury]
    #10571986 - 06/25/09 04:14 PM (15 years, 6 months ago)

Oh I don't use lysol and I think it's gross spraying chemicals all over the house like that.  It's a disgusting product, bathing in chemicals is not healthy at all.

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OfflineProfessor Frink
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Registered: 11/02/08
Posts: 144
Loc: USA
Last seen: 6 years, 9 months
Re: How necessary is Lysol? [Re: tektonic]
    #10572264 - 06/25/09 05:00 PM (15 years, 6 months ago)

Sloppy sterile technique works for some, but why take the chance?

I don't see much of a difference using Oust inside of a glove box as opposed to just using a fine mist of water to cleanse the air every 10 minutes. The same goes for soaking a tub with 70% alcohol while your working with flame sterilization inside. Water itself effectively holds contaminants to the sides and floor while i do culture work, G2G, and multi-spore innoc's with gourmets and medicinals. A Paper towel stack works wonders to keep the equip dry that i put down after the mist.

My apartment is absolutely filthy and even using proper sterile procedure in a glove box the rate of contam from this and human error (hey, no one's perfect) combined, add up to about <8% with G2G, multispore, and petri work. I am proud to say i've never gotten bacterial contamination on all 50 jars and minuscule amounts on petri.

For beginners its extremely valuable to learn proper sterile procedure, keep track of progress, and identify molds and bacteria. By knowing your enemies here you can learn, from experience, how to combat all of them. Proper sterile work gives you more insight into the vectors of contamination than a clueless person with a jar and multispore syringe, in a massively unsterile environment, will ever gather in the same amount of time.


--------------------
Culture trade list

Wanted Culture list:

Panellus stipticus
Yellow Morel

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OfflineCarcass
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Registered: 02/15/09
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Re: How necessary is Lysol? [Re: fungusfeller]
    #10572308 - 06/25/09 05:09 PM (15 years, 6 months ago)

i heard that you can use ultraviolet light source running for hours before you start to work does the same with lysol. kill the mold spores in the air.

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InvisibleDoc_T
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: How necessary is Lysol? [Re: Carcass]
    #10572319 - 06/25/09 05:11 PM (15 years, 6 months ago)

Quote:

Carcass said:
i heard that you can use ultraviolet




UV is wicked dangerous. Be very damn careful if you use it.


--------------------
You make it all possible. Doesn't it feel good?

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OfflineIzangi
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Registered: 07/18/08
Posts: 479
Loc: In a field in FL
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Re: How necessary is Lysol? [Re: fungusfeller]
    #10572359 - 06/25/09 05:19 PM (15 years, 6 months ago)

i have a ritual of using a whole 5 dollar can of lysol on my room the closet and the outside hallway...then if I have any oust i do the same..I wait like 5 minutes then do my work..just to be on safe side..maybe a little ocd lol


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chris606 said:
some 1 told me to try long brown rice and water and vermiculite and pressure cook for 1 hour i did but nothing happen that is y was wonting to no if i need spores
RogerRabbit said:
Nothing gets more sterile than red hot.


All posts are intended for fictional or educational purposes only.

No statements in any post or message by myself should be taken as fact. Use of contained information or opinions may be in violation of local, provincial, and/or federal laws.

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Offlinefungusfeller
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Registered: 05/14/09
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Re: How necessary is Lysol? [Re: Izangi]
    #10572373 - 06/25/09 05:22 PM (15 years, 6 months ago)

:rockon:


--------------------


TIMMY PUKESPLASH

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Offlinetazz
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Registered: 04/21/09
Posts: 162
Loc: The Great White North
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Re: How necessary is Lysol? [Re: Nuggetpouch]
    #10572378 - 06/25/09 05:23 PM (15 years, 6 months ago)

I don't use Lysol or Oust or alcohol or bleach for inoculation. IMHO all that crap is unnecessary.

I have a smallish glove (still air) box with open holes on the side. The box gets quickly wiped down inside with a clean dishcloth wet with dishsoap and water, and left to sit for 5 minutes or so before I go to work.

I work in the kitchen, or sometimes in the computer room (living room with carpet). No special cleanup is done before inoculation.

I wear Tyvek sleeves and latex gloves. The gloves get doused with iso alcohol, rubbed all over them, at the start of work, and again if I think they need it during the job.

That's it. I wash my hands before I begin, but I don't shower or do anything else special. And my house is NOT super clean - I'm not much of a house cleaner - a cat lives here too, so lots of cat dander floating around. No significant contamination problems whatsoever so far (knock wood).

Edited by tazz (06/25/09 05:25 PM)

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Offlinedarkestmage
Medical Student / Ski Bum

Registered: 06/18/09
Posts: 195
Last seen: 11 years, 4 months
Re: How necessary is Lysol? [Re: fungusfeller]
    #10572412 - 06/25/09 05:31 PM (15 years, 6 months ago)

I don't worry a lot about sterilization and have not had any contams yet.  I have my fruiting chamber, which i put upside down on my table.  I spray the area down with 10% alcohol or lysol, and just put everything inside the fruiting chamber and use it as a shield/tipped glove box.

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Offlinextofury
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Re: How necessary is Lysol? [Re: darkestmage]
    #10575567 - 06/26/09 08:05 AM (15 years, 6 months ago)

ummm I hear of people discussing sterile technique and I'm willing to bet the most common mistake is that people will think putting alcohol all over their hands is fine and they don't realize that they need to wash themselves like the doctors on tv do.

There is a very specific way in which doctors wash themselves before prepping for surgery.

Wash right up to your elbows.  Dig your nails into the alcohol (and try to keep them trimmed and clean).  Rub the edges of your cuticle where it meets skin.  Curl your knuckles in to get rid of the cracks to wash there.  Start out doing the palms of your hands of course (forgot to mention that, there'll be more alcohol to get between your prints).
It is also smart to shave your arms... but you don't have to do that (still not a bad idea though, even a trim 'll help).

It's more important to be sterile before colonization, once you have a fair amount of solid myc and perhaps some patches of greyish-white (less dense) semi-colonized stuff, the cake should be much more resilient to contamination and you can relax a little (depending on individual conditions of course).

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Offlinextofury
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Re: How necessary is Lysol? [Re: xtofury]
    #10575590 - 06/26/09 08:11 AM (15 years, 6 months ago)

By the way, there is nothing wrong with handling alcohol or bleach.  They are both safe chemicals for sterilization in my book, and I use them alot for cleaning.  You probably shouldn't use methyl alcohol though, lol.  The iso is fine.  Methyl has gradual skin contact toxicity, and h2o2 is a mutagenic so it's not the best thing to handle either (but not as bad as methyl alcohol).

Personally I would rather use food-grade ethyl but that can get a little expensive.

Maybe looking up msds before handling something is a wise precaution eh?

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OfflineCarcass
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Registered: 02/15/09
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Re: How necessary is Lysol? [Re: Doc_T]
    #10576016 - 06/26/09 10:26 AM (15 years, 6 months ago)

Quote:

Doc_T said:
Quote:

Carcass said:
i heard that you can use ultraviolet




UV is wicked dangerous. Be very damn careful if you use it.



thanks for the warning bro. meanwhile i'm OK with my sanitized glovebox.

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