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gus330
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Registered: 04/27/09
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Loc: south
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91% ISO alcohol
#10565795 - 06/24/09 04:23 PM (15 years, 6 months ago) |
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I was wondering does 91% ISO alcohol sterilize better than 70% ISO
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mrHerro
resistant to acid corrosion




Registered: 03/03/09
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Re: 91% ISO alcohol [Re: gus330]
#10565912 - 06/24/09 04:38 PM (15 years, 6 months ago) |
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Can't find the exact post but I remember reading one by a trusted cultivator that 70% will sanitise better. Apparently because the mixture does not evaporate as fast, leaving more time for the alcohol mixture to sanitise the surface.
someone please correct me if I am wrong
Edited by mrHerro (06/24/09 04:39 PM)
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RoosterCogburn
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Re: 91% ISO alcohol [Re: mrHerro]
#10565917 - 06/24/09 04:39 PM (15 years, 6 months ago) |
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70% is best.
The 30% water allows it to be absorbed into cells, and kill them.
91/99% is better for ISO hash and extraction stuff.
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Babo911
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the only difference i have ever found is how fast it evaporates.
I mix the two in 1:1 ratio.
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RoosterCogburn
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Re: 91% ISO alcohol [Re: Babo911]
#10565940 - 06/24/09 04:44 PM (15 years, 6 months ago) |
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It has to do with how well a contam can absorb the alcohol... 91% can't be absorbed, so it doesn't work.
They add water, to make it absorbable... if that's a word.
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hyphae
born to grow



Registered: 12/13/02
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70% is better because it doesn't evaporate as quick this means longer contact with the alcohol that is the only reason.
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Not what is overlay but rather what overlay is
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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RoosterCogburn
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Re: 91% ISO alcohol [Re: hyphae]
#10566386 - 06/24/09 06:22 PM (15 years, 6 months ago) |
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Quote:
Alcohols, usually ethanol or isopropanol, are sometimes used as a disinfectant, but more often as an antiseptic (the distinction being that alcohol tends to be used on living tissue rather than nonliving surfaces). They have wide microbicidal activity, are non corrosive, but can be a fire hazard. They also have limited residual activity due to evaporation, which results in brief contact times, and have a limited activity in the presence of organic material. Alcohols are more effective combined with purified water—70% isopropyl alcohol or 70% ethyl alcohol is more effective than 90% alcohol, because the higher water content allows for greater diffusion through the cell membrane. [3] Alcohol is, however, not effective against resistant fungal and bacterial spores.
- FDA/CFSAN - Food Safety A to Z Reference
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hyphae
born to grow



Registered: 12/13/02
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Quote:
RoosterCogburn said:
Quote:
Alcohols, usually ethanol or isopropanol, are sometimes used as a disinfectant, but more often as an antiseptic (the distinction being that alcohol tends to be used on living tissue rather than nonliving surfaces). They have wide microbicidal activity, are non corrosive, but can be a fire hazard. They also have limited residual activity due to evaporation, which results in brief contact times, and have a limited activity in the presence of organic material. Alcohols are more effective combined with purified water�70% isopropyl alcohol or 70% ethyl alcohol is more effective than 90% alcohol, because the higher water content allows for greater diffusion through the cell membrane. [3] Alcohol is, however, not effective against resistant fungal and bacterial spores.
- FDA/CFSAN - Food Safety A to Z Reference

Interesting I stand corrected there is another reason it is more effective haven't heard that one before. I know alcohol is quite absorbent threw the skin didn't realize water aids this diffusion thanks for the lesson RC!
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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veda_sticks
Cultivator




Registered: 07/29/07
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Re: 91% ISO alcohol [Re: hyphae]
#10566760 - 06/24/09 07:42 PM (15 years, 6 months ago) |
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I keep telling people that and theres 1 member here that calls me out bullshit.
70% alcohol works more effectively than 90% as 1, 90% evaporates to quickly, at most it dries out the cell making it dormant till it gets rehydrated. 2, the higher water content if 70% allows the mixture to be absorbed into the cell which is what kills it. Also alcohol doesnt kill alot of things, like bacillus and mould spores.
That is why i always say you shouldn;t have the need to wipe a flame sterilised syringe. And that dipping a needle in alcohol is no wear neer as effective as flaming. Granted that a syringe needle might not harbour bacteries etc, but theres always the chance something has worked its way inside the needle.
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gus330
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Registered: 04/27/09
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Loc: south
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i read a mono tub tek were they said to use vinegar before you use ISO to sterilize what is the purpose of that
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veda_sticks
Cultivator




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Re: 91% ISO alcohol [Re: gus330]
#10566835 - 06/24/09 07:56 PM (15 years, 6 months ago) |
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Quote:
gus330 said: i read a mono tub tek were they said to use vinegar before you use ISO to sterilize what is the purpose of that
IPA does not sterilise
--------------------
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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Nikon
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Registered: 01/29/09
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When you guys talk about 70% are you talking about methylated spirt? (Denatured alcohol)
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: 91% ISO alcohol [Re: Nikon]
#10566968 - 06/24/09 08:23 PM (15 years, 6 months ago) |
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Quote:
Nikon said: When you guys talk about 70% are you talking about methylated spirt? (Denatured alcohol)
That's the UK equivalent. We use isopropyl in the US, but you all use denatured ethanol. Works about the same.
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Nikon
Stranger


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Re: 91% ISO alcohol [Re: Doc_T]
#10567027 - 06/24/09 08:35 PM (15 years, 6 months ago) |
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I just wonder because I can get ISO from work, or buy it. But its damn expensive and in small bottles. Metho on the other hand, the natives here drink the stuff every store sells it.
So the sterilization properties are sufficient yeah?
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RogerRabbit
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Re: 91% ISO alcohol [Re: Nikon]
#10567990 - 06/24/09 11:35 PM (15 years, 6 months ago) |
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It has nothing to do with how fast the alcohol evaporates.
It has to do with pure alcohol not being admitted inside the cell walls by osmosis. If water is mixed with the alcohol, it tricks the cell wall into admitting it, therefore killing the cell. 70% has been shown to be a good mixture for sanitizing, and superior to higher concentrations. RR
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Don't rule out contact time it is also important. I read a good medical article years back that stressed this. I'll see if I can locate it along with an article on how effective vinegar/alcohol is at disinfecting.
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
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Re: 91% ISO alcohol [Re: hyphae]
#10569408 - 06/25/09 06:44 AM (15 years, 6 months ago) |
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isnt denatured alcohol what is used in alcohol lamps? which is a very high concentration? 99% which is why it burns with a very clean flame.
You can get 70% IPA in the UK. its just much harder to get. Only place i found is maplin. They sell it in a pressurised 400ml can for £7.99 or in a 1 litre can for 10.99
--------------------
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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DeeGee
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Registered: 06/25/09
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Interesting, I didn't know it was as scientific as that. I knew 70% was plenty, and disinfected at least the same as 91%, but 91% was only for people who want to look cool by using overkill.
-------------------- DG
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Brennus
Student of Life



Registered: 05/31/08
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Quote:
veda_sticks said: isnt denatured alcohol what is used in alcohol lamps? which is a very high concentration? 99% which is why it burns with a very clean flame.
Yep, denatured alcohol is used for alcohol lamps and stoves. I have a can of it sitting in my basement that I use to refill my alcohol lamps.
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RoosterCogburn
Fearless,one-eyed U.S.Marshall



Registered: 08/25/06
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Re: 91% ISO alcohol [Re: DeeGee]
#10570573 - 06/25/09 12:34 PM (15 years, 6 months ago) |
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Quote:
DeeGee said: but 91% was only for people who want to look cool by using overkill.
Or people looking to make ISO hash, and they don't want 30% water in there (water absorbs nasty shit, and not THC). In fact, I found 99% for such things.
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