Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Mono Tub Substrate   North Spore Bulk Substrate

Jump to first unread post Pages: < First | < Back | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Strain/Race/Variety Thread [Re: libertaire]
    #13198232 - 09/15/10 08:40 AM (13 years, 4 months ago)

Quote:

libertaire said:

You're right though, the way you described it would be the best way to achieve a desired trait, but you would also have to add the step of taking the clone of an isolated fruit and a clone of another isolated fruit, both the desired traits you'd like to combine, put them on agar, and let them meet to form their own sector or sectors.  Then isolate those sectors, grow each one out, and you should have the desired cross breeding.



There is alot more to it than this.  The two sectors will repel each other and then your back to the drawing board.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery
Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Strain/Race/Variety Thread [Re: andymc]
    #13198286 - 09/15/10 08:55 AM (13 years, 4 months ago)

Quote:

andymc said:
Quote:

LiquidMyce said:
Strains of Pleurotus specie(Oyster Mushrooms) are as follows;
Pink, blue, elm, yellow, ect.




Pink Oyster: species: Pleurotus djamor
Blue Oyster: species: Pleurotus ostreatus
Elm Oyster: species: Hypsizygus ulmarius
Yellow Oyster: species: Pleurotus citrinopileatus

Not strains but species (plural).



Agreed, but you left out the rest of my statement.

Quote:

A multispore culture of cubensis or oysters can/will have many strains.




This though, don't you mean a MS culture can/will have many variances in genetics, not strains?


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
Invisibleandymc
cocoa beetles from zanzibar
Male User Gallery


Registered: 11/25/09
Posts: 2,395
Re: Strain/Race/Variety Thread [Re: hamloaf]
    #13198313 - 09/15/10 09:04 AM (13 years, 4 months ago)

Quote:

LiquidMyce said:
Agreed, but you left out the rest of my statement.




Sorry, didn't mean to quote you out of context.

Quote:

LiquidMyce said:
Quote:

A multispore culture of cubensis or oysters can/will have many strains.




This though, don't you mean a MS culture can/will have many variances in genetics, not strains?




No, I meant that (in the simple explanation) spores would germinate and grow monokaryotic strands of mycelium, those would meet others and mate to form strains of dikaryotic mycelium (typically, many many of them in a multispore culture).  I was using it in the proper sense, but that probably just confuses things in the "strains" thread. :shrug:


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Strain/Race/Variety Thread [Re: andymc]
    #13198316 - 09/15/10 09:05 AM (13 years, 4 months ago)

Quote:

andymc said:

No, I meant that (in the simple explanation) spores would germinate and grow monokaryotic strands of mycelium, those would meet others and mate to form strains of dikaryotic mycelium (typically, many many of them in a multispore culture).  I was using it in the proper sense, but that probably just confuses things in the "strains" thread. :shrug:



So tru:lmafo:


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery
Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Strain/Race/Variety Thread [Re: truskool]
    #13198333 - 09/15/10 09:10 AM (13 years, 4 months ago)

Quote:

I was using it in the proper sense, but that probably just confuses things in the "strains" thread. 





:wink:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
Offlinelibertaire
liberator
Male


Registered: 08/06/08
Posts: 4,204
Last seen: 3 years, 6 months
Re: Strain/Race/Variety Thread [Re: truskool]
    #13198363 - 09/15/10 09:18 AM (13 years, 4 months ago)

Quote:

truskool said:
Quote:

libertaire said:

You're right though, the way you described it would be the best way to achieve a desired trait, but you would also have to add the step of taking the clone of an isolated fruit and a clone of another isolated fruit, both the desired traits you'd like to combine, put them on agar, and let them meet to form their own sector or sectors.  Then isolate those sectors, grow each one out, and you should have the desired cross breeding.



There is alot more to it than this.  The two sectors will repel each other and then your back to the drawing board.




Why would they repel each other?  Is it because they are dikaryotic?  I'll admit, I do have limited knowledge/experience in cross breeding, but this is just the way I had understood it, based on workman's strain crossing method.  I guess I forgot to take into account that dikaryotic mycelium does not mate with other dikaryotic mycelium.  Guess the only way is to put it under a microscope?  Is there another way to get them to mate?


Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Strain/Race/Variety Thread [Re: libertaire]
    #13198382 - 09/15/10 09:25 AM (13 years, 4 months ago)

Limited sucess has been acheived thru quatrenary ammonium 1/3 strength and rattlesnake venom to weaken the cell walls so that the dikaryotic strains will fuse instead of repeling


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Extras: Filter Print Post Top
Invisibleandymc
cocoa beetles from zanzibar
Male User Gallery


Registered: 11/25/09
Posts: 2,395
Re: Strain/Race/Variety Thread [Re: hamloaf]
    #13198399 - 09/15/10 09:30 AM (13 years, 4 months ago)

In case that last sequence of posts confused any noobs, I'm going to try to summarize with a metaphor:

Humans are a species of primate.  Macaques, howlers, marmosets, and mandrills are other unique species of primate that we like to call "monkeys".  We tend to imagine that we can tell different races of humans apart based on superficial attributes such as colour, size, shape of eyes, etc, so we give the races different names.

Substitution guide:
Human = cubensis
Primate = mushroom
Macaque, howler, marmoset, mandrill = blue, yellow, pink, and elm
Monkey = oyster
Race = "strain" (as in this thread)
Shape of eyes = shape of cap

For your convenience, here's are the substitutions completed:

Cubensis are a species of mushroomBlue, yellow, pink, and elm are other unique species of mushroom that we like to call "oysters".  We tend to imagine that we can tell different "strains" of cubensis apart based on superficial attributes such as colour, size, shape of cap, etc, so we give the "strains" different names.


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Strain/Race/Variety Thread [Re: andymc]
    #13198407 - 09/15/10 09:31 AM (13 years, 4 months ago)

Quote:

andymc said:
In case that last sequence of posts confused any noobs, I'm going to try to summarize with a metaphor:

Humans are a species of primate.  Macaques, howlers, marmosets, and mandrills are other unique species of primate that we like to call "monkeys".  We tend to imagine that we can tell different races of humans apart based on superficial attributes such as colour, size, shape of eyes, etc, so we give the races different names.

Substitution guide:
Human = cubensis
Primate = mushroom
Macaque, howler, marmoset, mandrill = blue, yellow, pink, and elm
Monkey = oyster
Race = "strain" (as in this thread)
Shape of eyes = shape of cap

For your convenience, here's are the substitutions completed:

Cubensis are a species of mushroomBlue, yellow, pink, and elm are other unique species of mushroom that we like to call "oysters".  We tend to imagine that we can tell different "strains" of cubensis apart based on superficial attributes such as colour, size, shape of cap, etc, so we give the "strains" different names.



:thumbup:
Well explained


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Extras: Filter Print Post Top
Invisibleandymc
cocoa beetles from zanzibar
Male User Gallery


Registered: 11/25/09
Posts: 2,395
Re: Strain/Race/Variety Thread [Re: truskool]
    #13198456 - 09/15/10 09:43 AM (13 years, 4 months ago)

Thanks Truskool.  Everybody likes monkeys.


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Strain/Race/Variety Thread [Re: andymc]
    #13198475 - 09/15/10 09:51 AM (13 years, 4 months ago)

Yes specially evil monkeys


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Extras: Filter Print Post Top
Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Strain/Race/Variety Thread [Re: libertaire]
    #13198480 - 09/15/10 09:52 AM (13 years, 4 months ago)

Quote:

libertaire said:

Why would they repel each other?  Is it because they are dikaryotic?  I'll admit, I do have limited knowledge/experience in cross breeding, but this is just the way I had understood it, based on workman's strain crossing method.  I guess I forgot to take into account that dikaryotic mycelium does not mate with other dikaryotic mycelium.  Guess the only way is to put it under a microscope?  Is there another way to get them to mate?




I've asked about this before and not gotten a good answer. What I dont quite get is when anastomosis occurs and where the genes originate that work their way into the spores.

I made up a substrate of 2 different spawns, PE and Costa Rica. The sub looked totally normal. No inhibition areas or weird patterns of growth. Costa has a much faster fruiting time and the sub fruited costa-looking fruits within a week of fruiting. A week later, the sub fruited PE looking fruits.

I asked about this and someone suggested that had I grown out some of the spores from those fruits, I could have possibly found a mixed phenotype. I never tried it or got confirmation on the theory.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


Extras: Filter Print Post Top
OfflineNizzyJones
Fight evil with funk
Male User Gallery


Registered: 08/22/06
Posts: 2,082
Loc: Somewhere North of Normal
Last seen: 1 year, 8 months
Re: Strain/Race/Variety Thread [Re: anonjon]
    #13198619 - 09/15/10 10:37 AM (13 years, 4 months ago)

You had two separate phenotypes cohabiting on one substrate. I don't know why that would lead anyone to believe they had exchanged DNA. :shrug:

To put it in a monkey analogy: that's like saying two monkeys sleeping next to each other (but not having intercourse) is enough for one of the monkeys to get pregnant.


--------------------
Wildflower seed on the sand and stone, may the four winds blow you safely home
Curriculum vapidum (dry herb vapes)


Extras: Filter Print Post Top
Invisibleandymc
cocoa beetles from zanzibar
Male User Gallery


Registered: 11/25/09
Posts: 2,395
Re: Strain/Race/Variety Thread [Re: NizzyJones]
    #13198674 - 09/15/10 10:51 AM (13 years, 4 months ago)

You draw them in with the monkeys, and then POW you hit them with the logic.


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Strain/Race/Variety Thread [Re: andymc]
    #13198710 - 09/15/10 10:57 AM (13 years, 4 months ago)

Quote:

andymc said:
You draw them in with the monkeys, and then POW you hit them with the logic.


:laugh2::rofl2:


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Extras: Filter Print Post Top
Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Strain/Race/Variety Thread [Re: NizzyJones]
    #13198864 - 09/15/10 11:33 AM (13 years, 4 months ago)

Quote:

NizzyJones said:
You had two separate phenotypes cohabiting on one substrate. I don't know why that would lead anyone to believe they had exchanged DNA. :shrug:






Well right, it doesn't seem like it could be true because something weird would have to happen where both strains were present in fruitbodies even tho they took one form or the other. Because I think anastasmosis occurs up in the gills at the basidia, but I guess it would be possible that nuclei from the other strain were somehow getting shunted up into the gills. Which sounds doubtful, but with as much as the mycelia likes to shuffle nuclei around, it's not impossible.

But lets say it's not possible. Then the 2 strains are cohabiting the same substrate as 2 distinct organisms. This seems more likely. But it's interesting that the diff strains don't inhibit each other or form any distinct zones like you expect them to on agar. You also don't see any more or less metabs on a multispore substrate than you do on an isolated strain. There doesn't appear to be much intra-species competition.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


Extras: Filter Print Post Top
Invisible13shroomsM
Lightning Shaman
I'm a teapot User Gallery


Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
Trusted Cultivator
Re: Strain/Race/Variety Thread [Re: anonjon]
    #13198946 - 09/15/10 11:46 AM (13 years, 4 months ago)

Quote:

Well right, it doesn't seem like it could be true because something weird would have to happen where both strains were present in fruitbodies even tho they took one form or the other




thats exactly what a grow from multispore does. :mushroom2:

thats what isolating to a monoculture is for, so you have what you want without as many possible variables.:thumbup:


--------------------
:takingnotes:A M U:helpdesk:
Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
"Man is the sex organ of the machine world" ~  Marshall McLuhan


Extras: Filter Print Post Top
Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Strain/Race/Variety Thread [Re: 13shrooms]
    #13198967 - 09/15/10 11:49 AM (13 years, 4 months ago)

Quote:

13shrooms said:
Quote:

Well right, it doesn't seem like it could be true because something weird would have to happen where both strains were present in fruitbodies even tho they took one form or the other




thats exactly what a grow from multispore does. :mushroom2:




it was just 2 isolates tho that looked very distinct from one another and came in waves of one phenotype or the other. u still think both strains were present in the fruitbody?


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Strain/Race/Variety Thread [Re: anonjon]
    #13199087 - 09/15/10 12:14 PM (13 years, 4 months ago)

Id put money on it that you got a flush of cr then a flush of pe.  Hence the wait in between flushes and the fruits looking different.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Strain/Race/Variety Thread [Re: truskool]
    #13199090 - 09/15/10 12:15 PM (13 years, 4 months ago)

^^^ that.


--------------------
You make it all possible. Doesn't it feel good?


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | Next > | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Mono Tub Substrate   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* THE STRAIN THREAD!
( 1 2 3 4 ... 21 22 )
Magician26 435,735 422 09/21/07 03:04 PM
by 2012shaman
* Words in the wind SixTango 13,906 9 05/13/03 02:39 PM
by Robbyrob
* Ultimate Myco Directory v5.0
( 1 2 all )
ChromeCrow 49,858 26 08/29/04 06:22 AM
by Anno
* Ultimate Mycodirectory v4.0
( 1 2 3 4 all )
oOjonahOo 270,732 64 03/06/05 09:48 AM
by Anno
* Oven Tek for Inoculation Jammer 14,393 15 04/06/19 04:05 PM
by bodhisatta
* glove box or oven tek? mellojello 5,954 15 04/07/19 08:12 AM
by bodhisatta
* Filter Patch bags VS. Canning jars for substrate
( 1 2 3 all )
Ryche HawkV 27,570 40 01/24/19 01:27 PM
by bodhisatta

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit
625,567 topic views. 0 members, 0 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.051 seconds spending 0.024 seconds on 15 queries.