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Offlinehamloaf
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Re: Strain/Race/Variety Thread. [Re: anonjon]
    #12928845 - 07/21/10 08:06 AM (13 years, 6 months ago)

If you want powerful while keeping well with in the growth parameters for cubes.  Grow Pan Cyans.  I can't attest to their potency personally.  To tell you the truth, I have never before heard of Pan Cyans until I joined the shroomery and began to lurk.  I have done alot of research into Pan Cyans and, from what I gather, Pan Cyans grow and can be stored like cubes.

I recently received my Pan Cyan MS syringe from Ralphster's and have inoculated LC's and agar plates with it.  I will have Pan Cyans in a couple of months.  Oh yes, I will.
HL


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleJavadog
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Re: is it worth it in the long-run [Re: hamloaf]
    #12928877 - 07/21/10 08:21 AM (13 years, 6 months ago)

Right on Ham.

I chose Pan-Cyan as one of my first set of strains.

I spawned three huge grain (rye) bags to poo+verm in seven
gallon milk jug tubs, one shoe-box sized tub, and my first
monotub (70 Qt)....all about a week ago,

I will report on what comes of it.  (and must put the
finishing touches on the now-dry martha that they are
incubating in)

I would add for the OP that another strain recognized as
powerful is the Azurescens....but I only mention it academically,
as it is a cold, wood-loving, strain and is not easy to
grow, from all reports.

Take care,

JD

(P.S. I never heard from you and chose Jalisco ;0)


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org


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InvisibleDoc_T
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Re: is it worth it in the long-run [Re: Javadog]
    #12928916 - 07/21/10 08:30 AM (13 years, 6 months ago)

Quote:

Javadog said:
I chose Pan-Cyan as one of my first set of strains.
I would add for the OP that another strain recognized as
powerful is the Azurescens....




These are species, not strains.


--------------------
You make it all possible. Doesn't it feel good?


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Invisibleanonjon
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Registered: 11/03/08
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Re: is it worth it in the long-run [Re: Javadog]
    #12929433 - 07/21/10 10:47 AM (13 years, 6 months ago)

Quote:

Javadog said:
Right on Ham.

I chose Pan-Cyan as one of my first set of strains.

I spawned three huge grain (rye) bags to poo+verm in seven
gallon milk jug tubs, one shoe-box sized tub, and my first
monotub (70 Qt)....all about a week ago,

I will report on what comes of it.  (and must put the
finishing touches on the now-dry martha that they are
incubating in)

I would add for the OP that another strain recognized as
powerful is the Azurescens....but I only mention it academically,
as it is a cold, wood-loving, strain and is not easy to
grow, from all reports.

Take care,

JD

(P.S. I never heard from you and chose Jalisco ;0)




There arn't enuff grow logs on pan cyan. You should log it!


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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InvisibleJavadog
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Re: is it worth it in the long-run [Re: anonjon]
    #12931581 - 07/21/10 05:53 PM (13 years, 6 months ago)

Anon,  you are right.  Any goodness will be shared.

I did find a couple of good threads... (Shea, was that you?)
..but I will post what I can whenever possible.

I am SO making this stuff up as I go, I hesitate to expose
newbies to my thought process before I have confirmed results.

Not that I am afraid of failure (see my "DOOMED" threads ;0)

I have noticed a few things about P-C...i.e. it can react
harshly to being shaken.  Also, I found that waiting for
every rye berry to look like a fuzzy white caterpillar
means waiting too long. (It took pooling metabolites to
force my spawn action)

I did get access to a camera that should make posting
a log possible.

So, good point.  Duly noted.


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org


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InvisibleJavadog
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Re: is it worth it in the long-run [Re: Javadog]
    #12931643 - 07/21/10 06:08 PM (13 years, 6 months ago)

P.S. The tubs are colonizing slowly, or so it seems to me,
but they are about 60-70% colonized and the few that I have
checked closely had no contams.

Are there threats introduced by agitating condensation on a
plastic wrap cover (with micro-pore taped hole cut in it)
so that it runs down?

This suggests another question...and a basic one, but I want
to confim...I have read teks that suggested leaving breathing
holes in spawned containers and others that answer slow
growth issues with "CO2 was too low.  Did you tape the lid?"

Q: When does one want zero GE, if ever?


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org


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Invisibleanonjon
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Re: is it worth it in the long-run [Re: Javadog]
    #12932742 - 07/21/10 09:17 PM (13 years, 6 months ago)

a little ge is fine


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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OfflineCounterstriker
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Re: Strain/Race/Variety Thread. [Re: Rose]
    #12933400 - 07/21/10 11:44 PM (13 years, 6 months ago)

Do B+ spawn well in mycobags , I hear some strains work better than others invitro.


--------------------
-CS

"All the years combine.. they melt into a a dream"


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Invisible13shroomsM
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Re: Strain/Race/Variety Thread. [Re: Counterstriker]
    #12933448 - 07/21/10 11:57 PM (13 years, 6 months ago)

:yesnod:

:kenthumbup:


--------------------
:takingnotes:A M U:helpdesk:
Click here ^ for the AMU forum
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OfflinePrimalSoup
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Re: is it worth it in the long-run [Re: Javadog]
    #12933537 - 07/22/10 12:28 AM (13 years, 6 months ago)

Quote:

Q: When does one want zero GE, if ever?




I can't think of anytime, except storage.

Peace
-PS


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InvisibleJavadog
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Re: is it worth it in the long-run [Re: PrimalSoup]
    #12934553 - 07/22/10 09:16 AM (13 years, 6 months ago)

Thanks all!


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org


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Offlineirockesp
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Which one to start with? [Re: Rose]
    #12936136 - 07/22/10 03:33 PM (13 years, 6 months ago)

I am a first time grower and ordered 5 spore syringes of cubes
I have Amazon, Ecuador, Lipa Yai, Transkei, and a Wollongong syringe.

I am very intrigued with strain isolation and agar work. I have made it before but with wild mushrooms from my lawn just for shits.

My question is which strain should I pick to begin to work with isolates?
I don't care if i should just knock up BRF or PF jars because I am what you call a begginer, it does not interest me as much as agar.


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Invisiblejokefox
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Re: Which one to start with? [Re: irockesp]
    #12936182 - 07/22/10 03:41 PM (13 years, 6 months ago)

Quote:

irockesp said:
I am a first time grower and ordered 5 spore syringes of cubes
I have Amazon, Ecuador, Lipa Yai, Transkei, and a Wollongong syringe.

I am very intrigued with strain isolation and agar work. I have made it before but with wild mushrooms from my lawn just for shits.

My question is which strain should I pick to begin to work with isolates?
I don't care if i should just knock up BRF or PF jars because I am what you call a begginer, it does not interest me as much as agar.




I got this guys

its good to start a jar to pick something to clone and isolate.
the problem with blind isolation is you have no idea on a profile** for the given "strain/sub strain/race"
when you start from a fruit , from a cake or tub or whatever , then you have an idea on a profile
you can check potency , if its a large fruit then you can isolate a larger fruit, or you can grab a nice small premature pin from a huge cluster and isolate a pinnset,  when you used mushrooms from your lawn you had a profile,
trust me , when you don know the life cycle of a mushroom , agar work is blind, pointless and can be frustrating if you wasted a long time on isolating a very weak mushroom isolate.

*profile,(very crude general meaning)- spores or sporeless, big or small fruits, potent or not, basically the general characteristic of the musrhoom


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Offlineirockesp
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Re: Which one to start with? [Re: jokefox]
    #12936283 - 07/22/10 03:57 PM (13 years, 6 months ago)

Jokefox,

You bring up very interesting points that help my understanding.

I had plans of using a few plates of agars and isolating some strains of each plate and growing those out until they don't sector and fruiting about 5 different strains. Would this be less blind?


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Invisiblejokefox
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Re: Which one to start with? [Re: irockesp]
    #12936316 - 07/22/10 04:02 PM (13 years, 6 months ago)

heres what i would do

i would use 10 jars ,
i would make 10 brf cakes

2 cakes of each kind

birth those cakes

take the 12 jars and use them for petri dishes 

then i would take one large fruit and clone it
and take one for pinset and clone it

thats the best way to get a full use out of your jars and mushrooms

i hope that helps

i ment blind as not being able to what you started working with
each spore is like a sperm , there the same but are some how different
you wouldent clone a human from sperm would you


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Offlineirockesp
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Re: Which one to start with? [Re: jokefox]
    #12936372 - 07/22/10 04:17 PM (13 years, 6 months ago)

Thank you so much for help Jokefox
Do you not recommend LC's?


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Invisibleanonjon
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Re: Which one to start with? [Re: irockesp]
    #12936501 - 07/22/10 04:44 PM (13 years, 6 months ago)

The reason he says you'd be isolating 'blind' and that you should fruit some cakes first is because you don't know what the fruits will look like until you fruit it. In fact don't even know for sure that you're isolating a fruiting strain.

The reason he suggests cakes first is because its the fastest way to get from spores to fruit.

LC's are awesome. But you want to isolate something worthy for your LC.

Each of these jars is agar with clone tissue from the fruit u see on top. They are from the same grow, but have diff genetics. The cloning was successful and I just transferred these to karo LC's.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


Edited by anonjon (07/22/10 04:48 PM)


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Invisiblejokefox
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Re: Which one to start with? [Re: anonjon]
    #12936679 - 07/22/10 05:26 PM (13 years, 6 months ago)

for lc's i recommend using agar

i use antibiotic Potato dextrose agar  diluted to about 1/5
so you get a broth
you can tweak it to how ever you like but make sure its more liquid like broth


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Offlineirockesp
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Re: Which one to start with? [Re: anonjon]
    #12937004 - 07/22/10 06:23 PM (13 years, 6 months ago)

Hmm you don't think if i tried to fruit 5 isolates atleast one would fruit?


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Invisibleanonjon
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Re: Which one to start with? [Re: irockesp]
    #12937032 - 07/22/10 06:27 PM (13 years, 6 months ago)

Quote:

irockesp said:
Hmm you don't think if i tried to fruit 5 isolates atleast one would fruit?




odds are that they all would. if you're intent on doing it in that order, go for it. The odds are you'll get some decent results.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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