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ezwider
Mushroom Enthusiast



Registered: 06/19/07
Posts: 971
Last seen: 2 months, 15 days
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Contamed wbs jars?
#10553368 - 06/22/09 12:22 PM (15 years, 6 months ago) |
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Well I never had a contaminated brf cake, however wbs has given me some problems.
I only did a 4-6 hour soak, and the water was still brown, so this may have something to do with it, but the brownish color I'm seeing in the jars might just be that brown water run off instead of a mold, so I would like to make sure.
I started out with 6 pints of creeper and 6 pints of burma.
I lost 3 creeper jars to contamination around 15% colonization.
The jars are now at 90%, and I have noticed 1 jar of creeper showing some funky signs, and 1 jar of burma showing signs as well.
The first two pictures are of the burma jar, at the top I can see a potentially moldy looking substance that might have a dark green hue but I might be wrong.
The last 3 photos are of the creeper jars, you can see the brown lining around the edges of the mycelium but no where else, maybe its just the brown from the poorly soaked wbs?
Thanks everyone.




-------------------- Proud to be a member of the Feel Family.
My Logs:
BRF Trial 1
First Mono Tub
Quadruple Mono Tub Grow
My Last Tub
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ezwider
Mushroom Enthusiast



Registered: 06/19/07
Posts: 971
Last seen: 2 months, 15 days
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Re: Contamed wbs jars? [Re: ezwider]
#10561329 - 06/23/09 08:21 PM (15 years, 6 months ago) |
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I dont see that blue/green part anymore, im just going to let them sit for a while longer and see if anything funky comes up.
-------------------- Proud to be a member of the Feel Family.
My Logs:
BRF Trial 1
First Mono Tub
Quadruple Mono Tub Grow
My Last Tub
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eric111e
Stranger

Registered: 02/21/08
Posts: 82
Last seen: 11 years, 5 months
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Re: Contamed wbs jars? [Re: ezwider]
#10562272 - 06/23/09 11:31 PM (15 years, 6 months ago) |
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That doesn't look like any wbs jar I've ever had. I would get rid of it, it just doesn't look right
Edited by eric111e (06/23/09 11:32 PM)
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Brennus
Student of Life



Registered: 05/31/08
Posts: 3,297
Loc: SE United States
Last seen: 6 years, 10 months
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Re: Contamed wbs jars? [Re: eric111e]
#10563428 - 06/24/09 05:51 AM (15 years, 6 months ago) |
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The milky, melted ice cream look is probably from a bacterial contamination. I'll wager those jars don't smell much like fresh mushrooms if you give them a whiff.
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Poseidon
My FAE is bigger than your FAE

Registered: 01/08/09
Posts: 229
Last seen: 14 years, 9 months
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Re: Contamed wbs jars? [Re: Brennus]
#10575612 - 06/26/09 08:19 AM (15 years, 6 months ago) |
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I agree with whoever said that doesn't look right. Kill it.
That being said I see browney/yellowing birdseed all the time. It is either bacteria or the result of fungicide that often comes with birdseed. Bacteria doesn't really fair too well on mushroom substrates, I have found, and usually just causes the mycelium to grow slower and realease a sort of mycelial urine making the jars look gross. Fungicide, which often comes with birdseed, does basically the same thing. Both of them cause them cause the mycelium to release nasty piss stuff but won't kill it. I've been able to remove both problems completely by a) Washing the birdseed very, very thoroughly, b) removing all floaters (they seem to cause the bacterial problems) and c) soaking for 24 hours, no less. It's all about giving your mycelium good quality, clean birdseed. Although funny enough the best quality stuff is the cheapest because it has less useless hydrophobic white millet in it.
-------------------- HELLO, NUBCAKES. ARE YOU HAVING CONTAMINATION ISSUES? WELL, YOU MUST NOT BE AWARE OF THE MAGICAL FORCE OF BACTERIAL ENDOSPORES. E-N-D-O-S-P-O-R-E-S. SPELL IT OUT, YOU GOD DAMN NUBLETS, BECAUSE IT WHAT CAUSES EVERY CONTAMINATION KNOWN TO MAN. ENDOSPORES HAPPEN WHEN YOU DO NOT PRESSURE COOK AT 15 PSI FOR AT LEAST 6 HOURS. LIKE A FUCKING VIETCONG IN HIS CAVE, THESE RELENTLESS COCKSUCKSERS CAN GET FUCKING FRIED AND STILL POP OUT OF YOUR GRAIN AND FUCK ALL YOUR SHIT RIGHT UP!! GETTING CONTAMINATIONS? WELL OBVIOUSLY, YOU DO NOT KNOW ANYTHING ABOUT ENDOSPORES!!
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Brennus
Student of Life



Registered: 05/31/08
Posts: 3,297
Loc: SE United States
Last seen: 6 years, 10 months
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Re: Contamed wbs jars? [Re: Poseidon]
#10575625 - 06/26/09 08:24 AM (15 years, 6 months ago) |
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The brown and yellow spots are likely metabolites. It's not "piss", if anything, it's closer to sweat. Biologically, they're a form of antibiotic used to fight infection. They are excreted usually when the mycelium is stressed - either from being overly colonized and waiting too long to spawn (oyster mushroom grain jars turn foul if you wait a week or two too long to spawn - there will be yellow metabolite pools at the top of the jar), being over-heated, or from fighting a competitor mold or bacterial infection.
The presence of fungicides wouldn't cause metabolites. It would either prevent spores from germinating, or simply do nothing if the WBS was inoculated with liquid mycelium. Floaters don't cause contamination, either - but it's still a good policy to toss most of them out because they waste space in your jars from being hollow.
I suggested tossing out the jars not because of the metabolites, but because one of the pictures has a milky, slimy appearance that usually indicates bacterial infection. Grain jars with metabolites are fine to spawn with if they pass the smell test, but doing a G2G with them is a bad idea.
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ezwider
Mushroom Enthusiast



Registered: 06/19/07
Posts: 971
Last seen: 2 months, 15 days
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Re: Contamed wbs jars? [Re: Brennus]
#10575976 - 06/26/09 10:14 AM (15 years, 6 months ago) |
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Here is the thing, its been a week now since I posted this, the jars are 100%, and I no longer see that milky white ice cream stuff you were talking about. It seems to have either been gobbled up byt he mycelium or just covered. I'm out of town for a week so I am going to check them again when i get back. I have pictures updated, but its just a plain old white mycelium jar that you guys wouldnt be able to do much with. Hopefully this week of waiting will let me know.
The brown stuff seems to be hanging out though, the water was still murky when I proceeded to pressure cook as I said before. Smell test when I get back.
-------------------- Proud to be a member of the Feel Family.
My Logs:
BRF Trial 1
First Mono Tub
Quadruple Mono Tub Grow
My Last Tub
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ezwider
Mushroom Enthusiast



Registered: 06/19/07
Posts: 971
Last seen: 2 months, 15 days
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Re: Contamed wbs jars? [Re: ezwider]
#10615270 - 07/03/09 11:11 AM (15 years, 6 months ago) |
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Well guys its been one week since I've been home and I came back to find this.
That they look basically the same with a slight brown discoloration. All of them look like this though, even the ones that obviously are not contaminated, has anyone ever gotten this brown discoloration in their wbs jars before, maybe from not thoroughly rinsing your wbs well enough? I'm spawning to a monotub today so I need to know this soon. Here are some updated pics.
This is one side of the 2 jars.
 Please note that the brown hasnt grown even after a week or two, so I'm beginning to think its not contaminated, but I need input.
Other side.

Here are the uncontaminated jars(hopefully) that are sitting in my closet away from the other two, they seem to look the same...

Let me know what you all think.
-------------------- Proud to be a member of the Feel Family.
My Logs:
BRF Trial 1
First Mono Tub
Quadruple Mono Tub Grow
My Last Tub
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