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OfflineMsGardener
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Did I mess up?
    #10552641 - 06/22/09 08:55 AM (15 years, 6 months ago)

After Sterilizing my jars, I only waited about an hour, and then I injected them with the spores. I thought the jars were cool enough, but I recently read about how the recommended wait time is 6-24 hrs! Do you guys think my spores are doomed :frown: ?




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OfflineCyber
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Re: Did I mess up? [Re: MsGardener]
    #10552644 - 06/22/09 08:56 AM (15 years, 6 months ago)

More than likely, yes. But you never know some may survive, I just would not hold my breath on that one. :wink:

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OfflineBrennus
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Re: Did I mess up? [Re: Cyber]
    #10552664 - 06/22/09 09:04 AM (15 years, 6 months ago)

:shrug:

It depends on how fast they were able to cool down. Thermal death for P. Cubensis spores is in the 115F-130F range, I think.

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OfflineMsGardener
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Re: Did I mess up? [Re: Cyber]
    #10552669 - 06/22/09 09:05 AM (15 years, 6 months ago)

This was about a day ago, I have more syringes.... Should I inject them again? just to be safe?? Thank Youuuuuu <3


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OfflineCyber
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Re: Did I mess up? [Re: MsGardener]
    #10552694 - 06/22/09 09:13 AM (15 years, 6 months ago)

It it was me, I would make up a new set. Leave the ones you have on the chance they grow but make a new set so you have some that were allowed to cool properly.

If you don't have enough jars or verm, brf, etc then you can reinject the jars. As I said, it would not me my first choice. All kinds of things can go wrong when trying to reinject so I avoid it where I can.

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OfflineDWreck
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Re: Did I mess up? [Re: MsGardener]
    #10552696 - 06/22/09 09:15 AM (15 years, 6 months ago)

wait and see what happens first. If nothing happens I would re-do the jars rather then inject again.
P.S. You should be able to get more then 5 jars from a syringe

Edited by DWreck (06/22/09 09:17 AM)

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OfflineMsGardener
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Re: Did I mess up? [Re: DWreck]
    #10552746 - 06/22/09 09:33 AM (15 years, 6 months ago)

Thanks soo much guys! I think I will wait a week before doing anything, then I will post some pics of the jars. I have 2 full syringes left, so this can be a learning experience I guess :]


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OfflineMsGardener
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Re: Did I mess up? [Re: MsGardener]
    #10552768 - 06/22/09 09:43 AM (15 years, 6 months ago)

^ and I have more than five jars incubating. I only have 5 in the pic ;]


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OfflineMsGardener
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Re: Did I mess up? [Re: MsGardener]
    #10588464 - 06/28/09 08:11 PM (15 years, 6 months ago)

4 of my babies are knocked up!!!!!!!!!!! Wahoooooooo :grin:


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OfflineHiddenIdentity
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Re: Did I mess up? [Re: MsGardener]
    #10588551 - 06/28/09 08:25 PM (15 years, 6 months ago)

On my first attempt, I waited about 1-2 hours.  After about a month, I threw the jars out because nothing was growing.  I am assuming they were too hot, but since I have yet to produce any shrooms, it could have been another reason - bad syringes, fungicides on the rye, something else...

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OfflineRoseM
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Re: Did I mess up? [Re: MsGardener]
    #10588553 - 06/28/09 08:25 PM (15 years, 6 months ago)

Congrats.

Patience is usually rewarded in this hobby.

:smile:


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Offlinehyphae
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Re: Did I mess up? [Re: Brennus]
    #10588572 - 06/28/09 08:29 PM (15 years, 6 months ago)

Quote:

Brennus said:
:shrug:

It depends on how fast they were able to cool down. Thermal death for P. Cubensis spores is in the 115F-130F range, I think.



122F (50C) :wink: Congrads MsGardener!!!


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