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Offlineapoonanor
Time & Space Traveler


Registered: 04/20/09
Posts: 380
Last seen: 9 years, 8 months
is a clone a single sector
    #10516495 - 06/16/09 11:38 AM (14 years, 8 months ago)

i figured i would bring this question to the adv. myc. forum because i've gotten mixed answers elsewhere (if this belongs in another place, mods, feel free to move it accordingly). i'm in the process of helping another member in a project to attempt to clone an albino fruit in order to try and produce an albino flush. at this point, the tissue has been cloned and placed on agar (which is quickly running as we speak). my question is, do these cultures represent single sector isolates or do they need to be isolated further in order to produce a single sector. it's purely a theorhetical question so i wasn't sure if it was something that could be answered theorhetically or if you would actually need to look at the plate to be sure. i'm still fairly new in this area so please excuse if this is a redundant question. any help would be greatly appreciated.


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Invisiblelipa


Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: is a clone a single sector [Re: apoonanor]
    #10516514 - 06/16/09 11:42 AM (14 years, 8 months ago)

If the culture is from a cloned piece of tissue no further selection is required as long as the culture is clean.

Lipa


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Offlineapoonanor
Time & Space Traveler


Registered: 04/20/09
Posts: 380
Last seen: 9 years, 8 months
Re: is a clone a single sector [Re: lipa]
    #10516516 - 06/16/09 11:43 AM (14 years, 8 months ago)

EXCELLENT. a somewhat simple answer. thank you very much.


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Offlinetalkingwalnut
Stranger


Registered: 10/03/08
Posts: 546
Last seen: 2 months, 24 days
Re: is a clone a single sector [Re: apoonanor]
    #10516534 - 06/16/09 11:48 AM (14 years, 8 months ago)

From my understanding more than 1 sector can still somtimes be in a fruit you clone.


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Offlineapoonanor
Time & Space Traveler


Registered: 04/20/09
Posts: 380
Last seen: 9 years, 8 months
Re: is a clone a single sector [Re: talkingwalnut]
    #10516546 - 06/16/09 11:51 AM (14 years, 8 months ago)

just out of curiosity then, given that the clone was taken properly, what is the probability the culture taken will produce the aforementioned albino flush without further isolation?


Edited by apoonanor (06/16/09 12:05 PM)


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Invisiblelipa


Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: is a clone a single sector [Re: apoonanor]
    #10516579 - 06/16/09 11:58 AM (14 years, 8 months ago)

Even if it sectors(looks like sectors) it is compatable hyphae. There is no worries here. It will produce just fine.

lipa


Edited by lipa (06/16/09 11:59 AM)


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Invisiblebosch
Registered: 01/16/09
Posts: 92
Re: is a clone a single sector [Re: lipa]
    #10516711 - 06/16/09 12:31 PM (14 years, 8 months ago)

In theory a fruiting body can have hundreds or thousands of strains co-existing in that one fruit.

Cloning from a fruit will probably get you the desired traits you want from the parent fruit, although rather closer to senesence than isolating from spores. This is not guarenteed, however, as there is still probably SOME genetic diversity in there.

If you're just trying to keep the albinism, then you are fine cloning from the fruit, whether it is a single-strain isolate or not, probably. If you actually want a single-strain isolate, unless you initially started your first project from one, you're almost certain to still have genetic diversity and thus it will probably sector out.


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Offlineapoonanor
Time & Space Traveler


Registered: 04/20/09
Posts: 380
Last seen: 9 years, 8 months
Re: is a clone a single sector [Re: bosch]
    #10516805 - 06/16/09 12:48 PM (14 years, 8 months ago)

here are some early pics of the myc growth. not sure if this will help or not but i figured i'd post them up. (sorry i'm still new to posting pics)

https://files.shroomery.org/files/09-25/517554475-albino_myc_agar_001.jpg

https://files.shroomery.org/files/09-25/517554491-albino_myc_agar_002.jpg

https://files.shroomery.org/files/09-25/517554509-albino_myc_agar_003.jpg

https://files.shroomery.org/files/09-25/517554543-albino_myc_agar_004.jpg

i also wanted to give "Time Ed" credit for these pics and this project. he is the one who found the albino and decided to try his hand at cloning for the first time. BIG thanks. it's been a learning experience for the both of us.

here's a link to "time ed's" original thread where he found the albino used to clone...
http://www.shroomery.org/forums/showflat.php/Number/10458834#10458834


Edited by apoonanor (06/16/09 01:00 PM)


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Invisiblebosch
Registered: 01/16/09
Posts: 92
Re: is a clone a single sector [Re: apoonanor]
    #10519008 - 06/16/09 07:07 PM (14 years, 7 months ago)

It's hard to say from those pictures, but it doesn't look like an isolated strain to me. They will almost certainly produce albino fruits, however.


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Offlineapoonanor
Time & Space Traveler


Registered: 04/20/09
Posts: 380
Last seen: 9 years, 8 months
Re: is a clone a single sector [Re: bosch]
    #10519124 - 06/16/09 07:21 PM (14 years, 7 months ago)

the albino fruits were the real goal. i was just curious about the isolate thing and it's relation to said albino fruits. much thanks.


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Offlinetroncotron
Stranger

Registered: 12/13/08
Posts: 79
Last seen: 11 years, 1 month
Re: is a clone a single sector [Re: apoonanor]
    #10538515 - 06/19/09 05:36 PM (14 years, 7 months ago)

you should get albino fruits by clonning, if the original fruit was a real albino. I mean if it carries the albino trait (mutation, alelic frequency...) in his DNA. I´ve recently had false albinos fruiting a thai multispore cake at low humidity levels.I cloned it and isolated seven diferent sectors and fruited them separately, but none of them gave any albino fruit.Otherwise, i could see clearly diferences between the substrains (isolated from the same specimen) in growth speed, pinning, etc







Good luck with the proyect:thumbup:


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