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Offlineneotekz
Stranger
Registered: 10/16/02
Posts: 8
Last seen: 13 years, 11 months
innoculation
    #1052533 - 11/14/02 03:16 PM (14 years, 24 days ago)

i innoculated a jar but how come only one out of the four points actually innoculated?
the jar is almost half filled with mycelium now but if this happens again can or should i try to innoculate the jar a second time or should i just leave it?


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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: innoculation [Re: neotekz]
    #1052539 - 11/14/02 03:19 PM (14 years, 24 days ago)

*inoculation, inoculated

you should just leave it. one is all you need. more just makes it faster. trying re-innoc may lead to contamination.


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: innoculation [Re: neotekz]
    #1052547 - 11/14/02 03:20 PM (14 years, 24 days ago)

Leave it alone , give it some more time .
What temps are your jars incubating in ?
If above or below the optimum range (86*F) it will be a bit slow .
Also was jar completly cool prior to inocing ?
If not hot spots may have occured , not to worry though , the myc you have will take over the jar .
Good Luck


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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OfflinepsilocybinjunkieM
relaxin
Male

Registered: 03/18/01
Posts: 13,322
Last seen: 8 hours, 49 minutes
Trusted Cultivator
Re: innoculation [Re: neotekz]
    #1052560 - 11/14/02 03:25 PM (14 years, 24 days ago)

That happens some time. My suspects are uneven spore distribution in the water. It takes longer to colonize when this occours. Thats on reason i prefer dark syringes.
May i ask where your syring came from? Homemade or vendor?


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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 11 years, 7 months
Re: innoculation [Re: Bilge]
    #1052564 - 11/14/02 03:28 PM (14 years, 24 days ago)

You corrrected him, then made the same mistake, thats great. But anyways, to the origional poster, just wait it out. You probably just had more spores in that one spot, but soon all will start to show growth.


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Offlineneotekz
Stranger
Registered: 10/16/02
Posts: 8
Last seen: 13 years, 11 months
Re: innoculation [Re: psilocybinjunkie]
    #1052582 - 11/14/02 03:33 PM (14 years, 24 days ago)

my jar was still kinda warm when i inoculated, it;s stored at around 21 degrees(70 degrees). where do u guys store your jars for it to be 86 degrees? i'm not about to go out and buy an incubator or something. i got my syring from a vendor. i know that i should wait but is it safe to try to inoculate the jar a second time if this happens again?
i'm not going to bother to inoculate this jar again cause it;s been around 3-4 weeks and the jar is half full


Edited by neotekz (11/14/02 03:34 PM)


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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: innoculation [Re: Skikid16]
    #1052588 - 11/14/02 03:35 PM (14 years, 24 days ago)

hehe. you are righ Skikid16!

my foot tastes delicious.


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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 11 years, 7 months
Re: innoculation [Re: neotekz]
    #1052599 - 11/14/02 03:37 PM (14 years, 24 days ago)

Incubators are cheap, easy, and very effective, I highly suggest you look into one
http://www.myco-tek.com/TEKs/Incubation.htm
http://www.fungifun.org/pf/incubator.htm

Both are great, the top link is better, IMO, just because its easier to set up and I think that styrofoam is a better insulator, but the bottom one is great because its big enough for casings, whereas its somewhat difficult to find a styrofoam cooler big enough for casings. What I do is keep my jars in the styrofoam cooler, and my casings in the rubbermaid. Works like a charm.


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Re-Defeat Bush in '04


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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 11 years, 7 months
Re: innoculation [Re: Bilge]
    #1052605 - 11/14/02 03:38 PM (14 years, 24 days ago)

No worries, oh doesn't crow go nicely with feet? hehehehehe, you still da man.


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Re-Defeat Bush in '04


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: innoculation [Re: neotekz]
    #1052850 - 11/14/02 04:59 PM (14 years, 24 days ago)

Cousin "IT" stores his above his oven in a cabinet , in summer temp is auto , in winter he turns it on(the oven) periodically to keep temps up . Is not consistent but is doing the trick . Can't wait for spring heat to come back ! So far average temp is 80*F , drops to 78 and spikes around 90 when oven is in use . Still with in the good range . :grin:
If jar was warm at time of inoc. , that may have killed mant spores preventing them from germinating , next time let jar sit till "room" temp .  :wink: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlineiloveraving
Fighting themachine...

Registered: 08/27/02
Posts: 1,965
Loc: ..long road ahead..
Last seen: 10 years, 2 months
Re: innoculation [Re: tripndicular]
    #1052909 - 11/14/02 05:14 PM (14 years, 24 days ago)

Do any vendors sell dark syringes?


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Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 11 years, 8 months
Re: innoculation [Re: iloveraving]
    #1052926 - 11/14/02 05:19 PM (14 years, 24 days ago)

the little guy sells dark syringes. Really reliable service, fast too. And cheap.


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OfflineAcidSmurf
Mycobandit

Registered: 10/24/02
Posts: 113
Loc: North of KY, South of MI,...
Last seen: 10 years, 9 months
Re: innoculation [Re: neotekz]
    #1053338 - 11/14/02 07:23 PM (14 years, 24 days ago)

True to that! The little guy is the SHIT! I ordered on friday evening a couple weeks ago and recieved them the following monday! Bought four syringes of creeper for thirty bucks. And also got a free syringe of mazatapec. Thanks little guy!


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If you drop your keys in a river of molten lava..... Let em go..... Because man... They're gone! - Jack Handey


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Amazon Shop for: ½ Pint Jars

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