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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Effects of Multispore transfer on bulk yields
    #1050420 - 11/13/02 08:23 PM (21 years, 4 months ago)

My lizard just pc'ed 7 quart jars of rye, and is planning to innoculate from a multispore syringe tomorrow. He plans to case the whole deal with poo later on, but has one worry.
All jars will be from syringe, rather than agar isolates. Anyone have any idea how this will affect the end yield? He's done the same with PF cakes and gotten good results, but just wants to be sure the varying strains won't greatly depreciate the outcome.
Any help would surely be appreciated.

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OfflineTheta
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Registered: 10/29/02
Posts: 352
Last seen: 18 years, 3 months
Re: Effects of Multispore transfer on bulk yields [Re: Servator]
    #1050463 - 11/13/02 08:35 PM (21 years, 4 months ago)

I have no real experience but I'm going to take a crack at it - it depends on the syringe. That is, it could vary widely depending on chance and the particular, possibly random contents of the syringe.

Most likely, yields will not be as great as they could be with an agar isolate.

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Offline420M
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Registered: 10/29/02
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Re: Effects of Multispore transfer on bulk yields [Re: Servator]
    #1050741 - 11/13/02 09:45 PM (21 years, 4 months ago)

Are you going to be using syringes of different strains?

~ 420M ~

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InvisibleServator
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Registered: 08/30/02
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Re: Effects of Multispore transfer on bulk yields [Re: 420M]
    #1050997 - 11/13/02 10:55 PM (21 years, 4 months ago)

No it's going to be the same strain. I was just worried that incompatibilities between seperate substrains might cause lower than usual yields.
Main reason for the concern was an article I was browsing at theomc.com mentioned that combining multispore jars could have adverse effects on the end result. It sounds kinda untrue to me, as I've done it before and had no trouble. I just thought I'd check some more knowledgable people to be sure.

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OfflineDERRAYLD
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Registered: 05/13/02
Posts: 9,760
Loc: South Africa
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Trusted Cultivator
Re: Effects of Multispore transfer on bulk yields [Re: Servator]
    #1051023 - 11/13/02 11:01 PM (21 years, 4 months ago)

I`ve used multispore innoculations many times with my bulk substrates and it deffinatelly hasn`t effected yield or size of fruit.
SO go for it.
I would however suggest rather innoculating one jar and perhaps doing g2g to the other 6 jars instead.

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