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Invisiblebob5

Registered: 03/01/09
Posts: 1,391
how many compartments.. in a petri dish?
    #10499898 - 06/13/09 02:03 PM (14 years, 8 months ago)

i'm just learning how to make a clone on agar...

what's best out of?
:
  • Single-Compartment Petri Dishes
    Two-Compartment Petri Dishes
    Three-Compartment Petri Dishes
    Four-Compartment Petri Dishes



is it riskier to use multi compartments..  -> due to the lid being opened more often?

cheers
b


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Invisiblebob5

Registered: 03/01/09
Posts: 1,391
Re: how many compartments.. in a petri dish? [Re: bob5]
    #10499952 - 06/13/09 02:17 PM (14 years, 8 months ago)

also - does anyone know where i can buy agar?
google is bogged down with other agars...:  there's a writer and some other shit.

i was searching the google uk.
what's the best nutrient(s) to add to the agar?




thanks alot.


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OfflineScavengerType
Male User Gallery

Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 10 years, 3 months
Re: how many compartments.. in a petri dish? [Re: bob5]
    #10500457 - 06/13/09 04:41 PM (14 years, 8 months ago)

Personally I like the O (one section) dishes for isolation particularly of sources like spores where you may have a large amount of genetic variation and will need not just one but also subsequent flowing isolations to reach a perfect isolate. In more isolated cases where not as much room is required, Y (three section) dishes are far better for low diversity isolation and can be used more easily for tissue transfers.

This is just my experience. I use a glovebox with good sterile procedure and have had contaminants in roughly 4% of my dishes only 1% of my dishes have ever had a working project in them while receiving a contaminant. So I would not worry so much about the amount of time open until you are actually working on the project.

I've heard complaints about the X (4 section) dishes being too constrained, but nothing about the I (2 section). I think I chose well to go with Os and Ys in the selection of my dishes and would recommend such a choice to anyone considdering the purchase of dishes. I may warn you that in order to get a good deal on dishes you often need to purchase around 300. In order to get the split of O and Y I had to go in with an associate here and got them at a cost of ~$.30 per dish.

As for agar, you'll need to find agar agar powder, many health foods stores stock this sort of thing I find. You may need to look arround a little more for a nearby seller.

The simplest additives are potato and dextrose (PDA), or malt extract(MEA). You can find a few of these recipes on the main page of the shroomery under the agar section.


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