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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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OfflineRainmann
Pimp
Registered: 11/13/02
Posts: 4
Loc: Texas
Last seen: 12 years, 1 month
Mycelium not growing
    #1049931 - 11/13/02 06:57 PM (14 years, 29 days ago)

Greetings,

I searched the FAQ and the archives and did not come up with any useful info. So, im posting here hoping for some insight.

It has been 14 days since innoculation of 12 jars with Golden Teacher. I have not seen any signs of activity whatsoever. not even an infection. The jars look the same now as they did two weeks ago.

my substrate (verm and brown rice flour) was sterilized in a pressure cooker. My syringe sat in the refrigerator for about a week before innoculation.

The jars cooled overnight before injection.

my incubator has maintained 80 degrees the entire time.

Could this be dead spores? Im not sure what else it could be since there are no signs of infection.... Thanks for any help in advance!


--Rainmann



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OfflineNew Name
New Name, OldUser
Registered: 11/11/02
Posts: 230
Loc: ATL
Last seen: 13 years, 11 months
Re: Mycelium not growing [Re: Rainmann]
    #1049946 - 11/13/02 07:05 PM (14 years, 29 days ago)

Could be bad spores, could be contamination that you can't see (bacterial). Did you inject the spores on the edge of the substrate where it meets the glass?


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"I will tell a cop that I know my fucking rights and we can match wits all night" -311
Don't let the cops search you. Let them know that you care about your rights.


Edited by New Name (11/13/02 07:07 PM)


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Mycelium not growing [Re: Rainmann]
    #1049947 - 11/13/02 07:05 PM (14 years, 29 days ago)

The only other thing I can think of would bacterial contamination. The signs are not always visible. Loosen one of the jar lids and take a careful sniff. Does it smell like sour vinegar?


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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OfflineRainmann
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Registered: 11/13/02
Posts: 4
Loc: Texas
Last seen: 12 years, 1 month
Re: Mycelium not growing [Re: Rainmann]
    #1049953 - 11/13/02 07:08 PM (14 years, 29 days ago)

Yes, I injected against the glass.
I just opened one and it doesnt have a nasty sour smell. These guys look just like they were just cooked. nice and clean. No sign of infection.


--Rainmann


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OfflineShrooms4e
LSAuser theScale Whore :)
Registered: 11/13/02
Posts: 160
Last seen: 14 years, 17 days
Re: Mycelium not growing [Re: Rainmann]
    #1049961 - 11/13/02 07:13 PM (14 years, 29 days ago)

order another syringe, actually whoever you ordered it from, state your complaint.


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OfflineRainmann
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Registered: 11/13/02
Posts: 4
Loc: Texas
Last seen: 12 years, 1 month
Re: Mycelium not growing [Re: Rainmann]
    #1049969 - 11/13/02 07:18 PM (14 years, 29 days ago)

I think ill do that. This just reaks of a bad syringe. Mucho Gracias.


--Rainmann


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: Mycelium not growing [Re: Rainmann]
    #1049999 - 11/13/02 07:35 PM (14 years, 29 days ago)

Weird. Have you taped the inoculation holes up? If so remove the tape. This tends to speed up the colonization but it's strange theres no sign of life after all that time


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineRainmann
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Registered: 11/13/02
Posts: 4
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Re: Mycelium not growing [Re: Bi0TeK]
    #1050011 - 11/13/02 07:42 PM (14 years, 29 days ago)

No tape... I just covered with aluminum foil and a rubber band. Very strange indeed. You would at least think there would be some sort of contamination showing.


--Rainmann


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Rainmann]
    #1050015 - 11/13/02 07:44 PM (14 years, 29 days ago)



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OfflineFlamDrag
I Like Pie
Registered: 10/26/02
Posts: 7
Last seen: 14 years, 12 days
Re: Mycelium not growing [Re: Rainmann]
    #1050056 - 11/13/02 08:01 PM (14 years, 29 days ago)

Maybe your brf was treated with fungicide. Is is organic? Have you been successful with the same brand of brf in the past? Some brands of brf do contain fungicide which will guarantee failure. If it's not the brf, it's the syringe.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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