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OfflineCyber
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Is this penicillin mold?
    #10449617 - 06/03/09 07:27 PM (14 years, 8 months ago)

Ok, This showed up in one of my plates and got me a little interested.







I took a sample of the yellow liquid that the mold seems to be producing, suspended it in an acetate solution,  and ran it through the spectrophotometer. It showed peeks at 314.00nm, 321.50nm, and 324.50nm which seems about right for penicillin. Most of the testing data states that it will absorb around 322.00nm but it is based on a purified product.

So does anyone know if this is penicillin mold?


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Offlineemstik5
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Re: Is this penicillin mold? [Re: Cyber]
    #10449826 - 06/03/09 08:00 PM (14 years, 8 months ago)

Looks like a form of Penicillium to me.

Has the macroscopic characteristics, white leading edge, banding etc...

Swipe some bacteria on that dish and see if it keeps away from the mould.

Got any pics through a microscope?

Have a look at
http://bioinfo.bact.wisc.edu/themicrobialworld/antimicrobial.html
There are some cool pics and a bit of info.


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InvisibleBlimeyGrimey
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Re: Is this penicillin mold? [Re: emstik5]
    #10450189 - 06/03/09 08:55 PM (14 years, 8 months ago)

Do you own your own spectrophotometer?


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OfflineCyber
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Re: Is this penicillin mold? [Re: BlimeyGrimey]
    #10450425 - 06/03/09 09:33 PM (14 years, 8 months ago)

Yes I do. As well as other things.


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InvisibleBlimeyGrimey
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Re: Is this penicillin mold? [Re: Cyber]
    #10450597 - 06/03/09 10:06 PM (14 years, 8 months ago)

Fucking awesome!

If I was to send you some reishi alcohol or acetone extract could you run a test for me?

I want to know the ganoderic acid content of my wild Puerto Rican Ganoderma.


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Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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Offlinemushroomhunter10
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Re: Is this penicillin mold? [Re: BlimeyGrimey]
    #10450663 - 06/03/09 10:17 PM (14 years, 8 months ago)

Hmm.
He lives near me.

Cyber, you wouldn't happen to have a tenure at OCC or or Wayne State would you?


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OfflineCyber
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Re: Is this penicillin mold? [Re: mushroomhunter10]
    #10452379 - 06/04/09 07:05 AM (14 years, 8 months ago)

Quote:

BlimeyGrimey said:
Fucking awesome!
If I was to send you some reishi alcohol or acetone extract could you run a test for me?





I would need 2x20ml samples of the ethanol extract and 1x20ml sample of the same ethanol that you used to make the extract, to run as a background. I could run it and send you the raw absorbency data but calculating the mg/g would be up to you. Drop me a PM and I'll see what we can do and discuss the sample preparation.

Quote:

mushroomhunter10 said:
Cyber, you wouldn't happen to have a tenure at OCC or or Wayne State would you?




mushroomhunter10, I am just an average guy off the street. :smirk: No tenure here. These are not the droids you are looking for, You can go about your business, move along. :lol:


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Offlinemushroomhunter10
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Re: Is this penicillin mold? [Re: Cyber]
    #10452428 - 06/04/09 07:31 AM (14 years, 8 months ago)

HeHe

I'm moving along!


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Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
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Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES


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