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Estario
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Registered: 05/01/08
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Honey LC Tek - pictorial 1
#10446142 - 06/03/09 06:21 AM (14 years, 7 months ago) |
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Honey LC Tek I had to make some new LC and decided I would share my method. Honey is a bit tricky to use as it caramelizes pretty easy, but this is the only additive I have had success with. With some caution during heat exposure I get pretty good LC jars without any sedimentation. I have used fructose, but the mycelium grew weak. So here it is. The material you will need: Organic Honey 20 ml syringe Mineral water 500 ml jars something to measure the amount of water used Proper jar lids (Jar lid with silicon injection port and polyfil filter (Tek) )
Step 1 Put some honey in a cup. My honey is very pure and I had to melt it down on a water bat. Don't use microwave as the honey will boil in just few seconds and you will have sedimentation, from crystals forming during the boiling.
 This is what it should look like, when ready for use:

Step 2 Take 11 ml of honey with a syringe.

Step 3 Use mineral water and measure 250 ml of it. Don't use tap water as it might be chemically treated. Tap water is being cleaned out of bacteria and fungi and it has additives to prevent them from contaminating it. You want exactly the opposite. You want fungi mycelium to grow in this water. Also mineral water carries its own energy.

Step 4 Mix the water and the honey in a 500 ml jar. If you use different amounts of water the ratio you are aiming at is 4 ml of honey per 100 ml of water (around 4 % honey solution).
 I warm the jars in a water bat to completely dissolve the honey.

Step 5 Put the lid on. I use lids with an air port and a self healing silicon injection port. They are pretty easy to make and provide real good air exchange as well as very good sealing after inoculation. Soon I will have to make some more and I will post a pictorial. EDIT: Here is the tek. Jar lid with silicon injection port and polyfil filter (Tek)

Step 6 Pressure cook your jars. First ensure they don't touch the bottom or the sides of the pressure cooker as the worm up and too high temperatures cause crystalisation. I use an old metal box to raise the jars above the bottom. Be creative.
 I put one layer of aluminum foil and PC for 15 minutes.
 Every 5 minutes I check the PC to see if it is boiling. When it starts boiling I turn down the heat and measure 15 minutes from that moment.
Edited by Estario (12/28/09 12:24 AM)
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Carcass
Polarized


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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10446154 - 06/03/09 06:30 AM (14 years, 7 months ago) |
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Thanks for sharing. Good pictorial.
   
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NoBrainNoPain
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Re: Honey LC Tek - pictorial [Re: Carcass] 1
#10448039 - 06/03/09 02:37 PM (14 years, 7 months ago) |
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How do you prevent your filter from getting wet when you shake your LC?
-------------------- I would love a woodlover's print… PM If you have one and want to trade
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Estario
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This is a ready LC done the same way, which I am using to inoculate the ready jars.
 This is how a jar should look like. There should be no sedimentations inside. If there are you should do the process again, because from my personal experience jars with caramelized honey are simple not being colonized.
 To draw LC from a jar you need to flame sterilize you needle.
 A syringe that is ready for use:
 About the shaking. I don't shake my jars that hard. Mycelium usually doesn't form so thick clouds with the setup I use and there is no real need. As for the speed of colonization, it is not so important for me as I always keep some LC ready for use. (1 jar in my incubator and 1 backup jar in the fridge) In case it gets contaminated or runs out I prepare some more with the backup LC and use it to inoculate any future projects until the new one is ready. I am doing this how pictorial, because my old LC got contaminated. I find this tek works for me perfectly. There are better solutions, providing more mycelium and faster colonization, such as using magnetic stirrer or potato LC, but I am a big fan of the KIS(keep it simple) idea. If something works well enough don't overtweak it.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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protestsong
City Fox


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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10448790 - 06/03/09 04:51 PM (14 years, 7 months ago) |
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how did you make your "alcohol lamp"? i'm pretty sure i know how... i just don't want to blow myself up or anything!
-------------------- "Everything you can imagine is real." - Pablo Picasso "Reality is merely an illusion, albeit a very persistent one" - Albert Einstein "He felt that his whole life was some kind of dream and he sometimes wondered whose it was and whether they were enjoying it." - Douglas Adams "Beauty is in the eye of the beholder, and it may be necessary from time to time to give a stupid or misinformed beholder a black eye." - Miss Piggy
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Estario
Enthusiast



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Re: Honey LC Tek - pictorial [Re: protestsong] 1
#10448897 - 06/03/09 05:10 PM (14 years, 7 months ago) |
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You simply drill a hole in the lid and use some cotton wool as a wick. Noting too fancy or complicated. The more wick you have sticking out, the bigger the flame will be.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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Estario
Enthusiast



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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10465642 - 06/07/09 12:23 PM (14 years, 7 months ago) |
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And here are the results 4 days later.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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Plastered marble
All posts fictional

Registered: 12/28/08
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Re: Honey LC Tek - pictorial [Re: Estario] 2
#10466646 - 06/07/09 04:25 PM (14 years, 7 months ago) |
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You're mycelium loves to make snowballs allright!
Also: did you know a lot of nail clippers have a little "hook" on the inside to scrape fingernails clean? ... just saying ...
-------------------- I survived operation midnight climax and all I got was really, really high. (older sigs)
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Estario
Enthusiast



Registered: 05/01/08
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Yep. I noticed the fingernail issue and took care of it. Right now I have exams and I seem to forget to do simple things such as cutting my nails.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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spoonman_
Stranger
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10469341 - 06/08/09 03:11 AM (14 years, 7 months ago) |
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Thanks for this, nice pictures.
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romulux
Connoisseur of Hallucination



Registered: 08/11/08
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Re: Honey LC Tek - pictorial [Re: spoonman_] 1
#10469532 - 06/08/09 05:28 AM (14 years, 7 months ago) |
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awesome tek, thanks dude!
-------------------- My psytrance: Soundcloud:Facebook
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Estario
Enthusiast



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Re: Honey LC Tek - pictorial [Re: romulux] 1
#10505561 - 06/14/09 04:39 PM (14 years, 7 months ago) |
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I have a question. Lately I have been reading All Of RR's Notes On Mushroom Cultivation Forum. It is something quite big and full of information. I have changed many aspects of my growing procedures. Recently I got to the part where RR speaks about LC. Apart from recommending against inoculating LC directly with spores he mentions a recipe for LC.(this is the better way of making LC)
Quote:
*Extra light malt, dextrose, nutritional yeast, gypsum. Makes the strongest growth. LC/AGAR
Now I have 3 questions. First one is: Can I use small amount of gypsum and nutritional yeast with honey? Is brewers nutritional yeast the same as the brewers yeast I take as vitamins? Second question. Dextrose is a sugar. I know that. But I am not sure if extra light malt is the same as pure maltose? Can one use coffee as additive to the LC to lower pH and add some nutritions to the mix? The water I use for LC has a pH of around 8,5.
Edited by Estario (06/16/09 09:45 AM)
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misfitdeviate

Registered: 06/20/09
Posts: 762
Loc: ESSR
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10572394 - 06/25/09 05:27 PM (14 years, 7 months ago) |
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hey, thanks for this tek, I did it for my first time about a week ago and everything has worked out great!
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Estario
Enthusiast



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I ran out of mineral water and I had to prepare 8 jars and PC them in 2 different pressure cookers. They all ended up with caramelized. I don't know what tap water in my area contains, but it definitely doesn't work for me.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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talane2005
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Re: Tap water Vs Mineral Water [Re: Estario] 1
#10735783 - 07/24/09 10:37 PM (14 years, 6 months ago) |
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very helpful post. had some pretty good growth, however, even with a small amount of sediments. but you're right, most of my jars that did contain the sediments simply didn't colonize.
great clear pictures. hopefully no more "what should finished LC look like" questions.
-------------------- See you later, Space Cowboy...
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Kaoskid
inspiring mycologist


Registered: 07/18/09
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Re: Tap water Vs Mineral Water [Re: talane2005] 1
#10735929 - 07/24/09 11:07 PM (14 years, 6 months ago) |
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im kinda new here and failed at my attempt to create a LC using karo. im now attempting honey. could you clarify the part when your pressure cooking them. you say you check it every five mins to see if its boiling and then you turn the heat down and pressure cook it for 15 mins. i tried that and my whistle never blew on my PC. so are you actually achieving the 15psi or what ever psi your going for, or are you just kind of "steaming them"?
and my first attempt might of failed due to my syringe. it was from spores101 and after i got some from elsewhere i could see how bad spores101 sucks. i ordered them prior to my research.
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Gnarly Tourettes
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Re: Tap water Vs Mineral Water [Re: Kaoskid] 1
#10736197 - 07/24/09 11:57 PM (14 years, 6 months ago) |
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i have an lc colinizing with a cat hair in it.. had to of been there before sterilizing maybe stick to the inside of the jar lid or something..
i just see it floating around in the myc like wtf stupid cat
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Estario
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Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive. Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
Edited by Estario (09/02/09 10:33 AM)
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MagicMaker
The Lizard King




Registered: 10/10/06
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Re: Early enzyme development in LC [Re: Estario] 1
#10987848 - 09/02/09 04:49 PM (14 years, 4 months ago) |
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Quote:
Estario said: Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive. Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.
interesting theory, sounds plausible, i have been doing research as I am going to make my first LC soon, this tek looks like a good one to try, my question is, to get the LC out after colonizing it do u just tip the jar as not to wet the polyfil and suck it up? i guess thats the only way,
also want to say you could probably just pull polyfil through a small hole instead of bothering to insert a tube and have to silicon it on both sides, might save you some time,
thanks for the alcohol lamp though thats a damn good idea, you think a peice of a shirt will work or does it have to be wool?
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Estario
Enthusiast



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Re: Early enzyme development in LC [Re: MagicMaker] 1
#10988032 - 09/02/09 05:20 PM (14 years, 4 months ago) |
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Quote:
MagicMaker said:
interesting theory, sounds plausible, i have been doing research as I am going to make my first LC soon, this tek looks like a good one to try, my question is, to get the LC out after colonizing it do u just tip the jar as not to wet the polyfil and suck it up? i guess thats the only way,
I just tip the jar a little bit and stick the needle inside. I don't let the edge of the LC touch the lid.
Quote:
also want to say you could probably just pull polyfil through a small hole instead of bothering to insert a tube and have to silicon it on both sides, might save you some time,
I prefer using the tube, because when shaking the jar the liquid flows all around and the tubes serves as a protection for the polyfil. Otherwise it would get wet. It holds the filter in place and also increases its thickness. Also it is much easier to replace the filter if needed once you have the tube installed.
Quote:
thanks for the alcohol lamp though thats a damn good idea, you think a peice of a shirt will work or does it have to be wool?
It has to be a material that can suck up alcohol. Some synthetic materials are not suitable for this purpose, but I think a shirt will work out. An easy test you can perform is soak a piece of the material in alcohol and then squize it. A small stream should come out, which means the material absorbs it and can serve as a wick.
Happy growing. This tek has worked out for me the best. Honey has carbohydrates, amino acids and vitamins which are all essential for the development of a healthy mycelium.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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MagicMaker
The Lizard King




Registered: 10/10/06
Posts: 626
Loc: Terrapin
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Re: Early enzyme development in LC [Re: Estario] 2
#10988080 - 09/02/09 05:27 PM (14 years, 4 months ago) |
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good point about the tubing that would seem to protect the filter from getting wet due to swirling, and def easier to replace, more air flow too but i bet just pulling through a hole packs it a lot tighter which would be better to keep out contam, but whatever floats ur boat i can def see wetting the polyfil being an issue
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Estario
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Gentamicin Sulfate Antibiotic LC [Re: MagicMaker] 1
#11143166 - 09/28/09 12:00 PM (14 years, 4 months ago) |
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I have been adding Gentamicin sulfate to my LC lately. I ad 80 mg of gentamicin to 1250 ml of LC. The mycelium grows as usual and I think those new jars are much more contamination resistant than my older ones. I will try inoculating one of those jars with contaminated LC to see if the bacterial growth is inhibited in those antibiotic LC's.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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vishal779
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Re: Gentamicin Sulfate Antibiotic LC [Re: Estario] 1
#11509248 - 11/23/09 07:17 AM (14 years, 2 months ago) |
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hi..just wanted to know if mineral water is the best for lc or distilled water and tap water are equally nice as they are finally going to be sterilised anyway..
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dman080
00000000000000000000000000000


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Re: Gentamicin Sulfate Antibiotic LC [Re: vishal779] 1
#11509265 - 11/23/09 07:26 AM (14 years, 2 months ago) |
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SOrry Visual dont know exactly about the mineral water. I did a Karo tek about 4 days ago. And I used Distilled water from a bottle. What I was going to say is mine does not look like that after 4 days. Its my second attempt with LC . No signs of anything but clear right now...
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Gentamicin Sulfate Antibiotic LC [Re: dman080] 1
#11509273 - 11/23/09 07:35 AM (14 years, 2 months ago) |
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Wait 7-14 days to see visible growth from spores.
-------------------- You make it all possible. Doesn't it feel good?
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myceleus_rex
seeker



Registered: 07/01/09
Posts: 1,581
Loc: alized
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Re: Early enzyme development in LC [Re: Estario] 1
#11509278 - 11/23/09 07:39 AM (14 years, 2 months ago) |
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Quote:
Estario said: Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive. Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.
As long as this thread is bumped:
This idea sounds intriguing. The only thing is, I'd think that the little bit of ground-up grain would be inadequately sterilized and could introduce contamination.
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dman080
00000000000000000000000000000


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Re: Gentamicin Sulfate Antibiotic LC [Re: Doc_T] 1
#11509280 - 11/23/09 07:41 AM (14 years, 2 months ago) |
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Quote:
Doc_T said: Wait 7-14 days to see visible growth from spores.
Oh ok cause this guy is like 4 days. But he prob use another LC . Thats what makes me love this hobby. Cause you can keep regenerating your stock. . So fucking cool...
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Gentamicin Sulfate Antibiotic LC [Re: dman080] 1
#11509291 - 11/23/09 07:49 AM (14 years, 2 months ago) |
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Quote:
dman080 said: Oh ok cause this guy is like 4 days. But he prob use another LC .
No. It doesn't matter what you are growing the spores on. They germinate when they are ready. Fresh spores may take off right away. But wait 7-14 days to see growth from random spores.
And stop staring at your LC. Every time you touch it or breathe on it, you are risking contamination.
-------------------- You make it all possible. Doesn't it feel good?
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dman080
00000000000000000000000000000


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Re: Gentamicin Sulfate Antibiotic LC [Re: Doc_T] 1
#11509296 - 11/23/09 07:52 AM (14 years, 2 months ago) |
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Quote:
Doc_T said:
Quote:
dman080 said: Oh ok cause this guy is like 4 days. But he prob use another LC .
No. It doesn't matter what you are growing the spores on. They germinate when they are ready. Fresh spores may take off right away. But wait 7-14 days to see growth from random spores.
And stop staring at your LC. Every time you touch it or breathe on it, you are risking contamination.
Nah i dont fuck with it .. i check it evry 2nd day to see if it started yet. Im pretty chill when it comes to waiting for these things. But when he said four days that rAIsed a Red flag. Thanks BTW..
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cacharstar
Strange is good...



Registered: 11/13/08
Posts: 4,014
Loc: The West Coast
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Re: Early enzyme development in LC [Re: Estario] 1
#11603541 - 12/07/09 01:46 PM (14 years, 1 month ago) |
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Quote:
Estario said: Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive. Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.

Would this improve the acceptance time of lc to wood lovers substrate as well? Also if a culture is taken from fruiting bodies or transfer from agar to agar from the wild, then the DNA should be unlocked right? This applies to germinated spores I assume?
--------------------
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Estario
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Re: Early enzyme development in LC [Re: cacharstar] 1
#11729409 - 12/28/09 12:37 AM (14 years, 1 month ago) |
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Quote:
cacharstar said:
Quote:
Estario said: Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive. Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.

Would this improve the acceptance time of lc to wood lovers substrate as well? Also if a culture is taken from fruiting bodies or transfer from agar to agar from the wild, then the DNA should be unlocked right? This applies to germinated spores I assume?
Paul Stamets explains this topic pretty extensively in his book Growing Gourmet and Medicinal Mushrooms. He recommends using the soak water from grains to make LC. I have stored some but right now I don't have the need for any more LC so I will use it later. If any one is interested in this topic read page 107.
Quote:
To ameliorate degenerative effects, the addition of extracted end-substrates (sawdust, straw, etc.) favors the normal development of mycehium. The introduction of the end-sub- strate acquaints the mushroom mycelium with its destined fruiting habitat, challenging the mycelium and selectively activating its enzy- matic systems. This familiarity with the end-substrate greatly improves performance later on. Parent cells retain a"genetic memory" passed downstream through the mycelial net- works. Paul Stamets - Growing Gourmet and Medicinal Mushrooms
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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santaclausmush
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#12217723 - 03/17/10 10:01 AM (13 years, 10 months ago) |
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Quote:
Estario said: And here are the results 4 days later.
in this tek did ur mycelium stay and grow at the bottom of the jar? i have six bottles already growing myc. in all of em. but they are all contammed. but they still grow bigger clouds everyday.
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Razzl3Frazzl3


Registered: 07/31/09
Posts: 4,630
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Thanks, how do i sticky this???
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kurshiukas
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Quote:
santaclausmush said:
Quote:
Estario said: And here are the results 4 days later.
in this tek did ur mycelium stay and grow at the bottom of the jar? i have six bottles already growing myc. in all of em. but they are all contammed. but they still grow bigger clouds everyday.
if by contam you mean mold, then the clouds you see are probably mold clouds. Mold myc is the white as shroom myc as long as it does not sporrate.
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santaclausmush
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Re: Honey LC Tek - pictorial [Re: kurshiukas] 1
#12218053 - 03/17/10 11:14 AM (13 years, 10 months ago) |
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this is my clouds. there white but im colorblind so i put em in front of my high pressure sodium grow light to see and there white. but i got contam on all of it. this is all good. some i have black dots in and they all are contamed but huge myc clouds it loooks like.
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Mountrakker
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#12230860 - 03/19/10 12:35 PM (13 years, 10 months ago) |
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hey thanks for this great tek, the lids look really prof now
i have done 2 Honey Lc jars by the book/tek but there was too much sediment according to my judgement, is there any chance the flowing around sediment would use as an anchorage for the mycelium? they haven't formed a dense blob as of yet, and they've been in there for more than a week
 i like the right jar more, it looks better, and it looks as if it has too types of sediment, the brown (caramelized) and tiny bits and clumps that have formed white hairs on them. The left one (short one) had too much sediment to begin with (more bio-honey) and i guess it will be discarded
what do you guys think?
now i've got bird seed and popcorn waiting to be pressure cooked and sterilized, as to test the formations in the honey Lc, do you guys think ill have any chance of success? im really new to Lc and in my country(europe) we haven't got Karo products although today at the supermarket i saw some artificial sweetener sugar with a malt-dextrose mix..frustrating..
peace out
-------------------- Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer
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Mountrakker
r2d2



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Re: Early enzyme development in LC [Re: Estario] 1
#12235563 - 03/20/10 06:03 AM (13 years, 10 months ago) |
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Okay, so the Honey Lc has too much sediment and i'm overconfident with what i do, i guess those two jars in my previous thread have nothing but sediment, and the spores can't get a grip
question : Sedimentation retarded mycelium from forming. The suspended spores should be still vital right?
in this pic i want to make Lc to Lc tranfer, havent done that before

the left one is the one with sediment + spores the right one is 4% honey properly done
has anybody had success with that? remember i want to do Lc to Lc but with no clear mycleium formation from the first, just the suspended spores (or clumps)
thanks
-------------------- Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer
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santaclausmush
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Re: Early enzyme development in LC [Re: Mountrakker] 1
#12235584 - 03/20/10 06:16 AM (13 years, 10 months ago) |
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Quote:
Mountrakker said: Okay, so the Honey Lc has too much sediment and i'm overconfident with what i do, i guess those two jars in my previous thread have nothing but sediment, and the spores can't get a grip
question : Sedimentation retarded mycelium from forming. The suspended spores should be still vital right?
in this pic i want to make Lc to Lc tranfer, havent done that before

the left one is the one with sediment + spores the right one is 4% honey properly done
has anybody had success with that? remember i want to do Lc to Lc but with no clear mycleium formation from the first, just the suspended spores (or clumps)
thanks
both jars if u inoculate them will grow a fluffy sediment in it... did u use distilled water... i like using distilled water when i make LC cause theres nothing in the water and it always looks clearer. so u can see the myc better.. but test the LC on one jar first before u go making all these jars then inoculate them and they all grew contaminated .this LC JAR is contaminated... those other pics are the white fluffy stuff in em but when i inoculated my stuff it was all contam...you get fluffy white stuff if its contammed or not... so b carefull so u dont waste all that time.. THE CLEARER HONEY U CAN FIND THE BETTER.USING DISTILLED WATER IS BETTER. ive tried 3 kinds of honey and all worked... just use a test jar to make sure its good...
ne thing elsee i can help u with.. he liquid in this jar i used to make LC with cause the liquid and the myc are 100% colonized so i sucked some liquid out of the jar and used it to make LC. then i took the LC and inoculated one jar with brf and it turned white white so no contam in that LC... make sure u dont contam ur LC JAR before, after or during inoculation.. then u wont know its contamed and all ur jars will be contamed.
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Mountrakker
r2d2



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hey thanks for the help, the last 2 hours i've been experimenting like crazy,
a) Lc to Lc b) Lc to Wild Bird Seed, and Popcorn ! (properly done with polyfill fae, and silicone inoculation points)
c) Alive Pf-tek cake mycelium (3cm x 3cm)(that has produced 3 flushes already) into honey Lc! (glovebox- inner part of cake with sharp knife)
d) Re-Inoculated 10 pf-tek jars after 20 days that show no activity, with honey lc in question (strange thing is that in the syringe that sucked up the honey lc although didnt contain any myc blobs, i couldn't see any typical black spores floating around, though i shot more that 5-6 cc of dense spore medium 10 days ago)
yes this time i used distilled water, you are right, there is tremendous change in my lc appearance and i hope this time it'll work
thanks for the help and guidance
-------------------- Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer
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santaclausmush
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Re: Early enzyme development in LC [Re: Mountrakker] 1
#12236297 - 03/20/10 10:48 AM (13 years, 10 months ago) |
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ur LC IS CONTAMINATED. 20days no show nothing CONTAMINATED. LC THE QUESTION U SAY NO.... contaminated.... the lc if contamed will still show blob of fluffy myc.... so u have to test on one or two jars to make sure. LC is a joke u cant tell if its good or bad. have to test i had eight LC jars. all of em were contaminated. best wishes and good luck..
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Mountrakker
r2d2



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Hey thanks for the tip, though i have to say that its my Pf-tek Jars that haven't colonized in 20 days, not my Lc that is why i shot them around 3-4 cc honey lc again to test..
it is highly probable that you are right and it is contamed, will know soon for sure though, time will tell as Bob Marley sais

hey the bird seed is really cool, popcorn is ok, rye berries is the last i haven't tried and i guess the best of them all
will keep this process posted once i get any results
tx!
-------------------- Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer
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santaclausmush
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Re: Early enzyme development in LC [Re: Mountrakker] 1
#12246958 - 03/22/10 04:29 AM (13 years, 10 months ago) |
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if u did the pf tek to spec and boiled or pc. it doesnt matter the lc wont make nothing sometimes if its bad on brf cakes... IF THE LC IS CONTAMED sometimes it wont show nothing u could have done the pf tek fine. just the LC is shit. where do u live? if ur looking to do organic rye berries watch out it could cost u some coin... i was doing organic rye berries for a while.. it was 20$for the bag and 30$ shipping so it was fifty a bag...
i found a coop in concord nh.. from a friend alientechkilla and now i use organic wheat berries at 1.29 a lb... and they work just as good.saved me alot of coin too.
i see u live in uk.. so look around for organic stores or some small store somewhere will sell the berries.. take care good luck and best wishes.
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Mountrakker
r2d2



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i think ill end up with a bag of Agar-agar powder and a series of petri-dishes just to be sure!the latest bummer is that the honey lc jars i made, created sediment 2 days after it was pc'ed..strange
i bought me organic rye berries for 1.50 euro (about 2 bucks) per 500gr of product(total rip-off) they look nice, but will not be touching them until i have solid happy white mycelium, ready colonize em like there's no tomorrow..
i live in southern europe in the country that invented the 12 gods of Olympus(not the camera-lol) and has now a huge deficit to balance out
you say organic wheat berries worked for you as well?? now thats interesting, wheat berries are much cheaper than rye.. share your experience! do you think they're better than rye?
thanks
-------------------- Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer
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santaclausmush
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Re: Early enzyme development in LC [Re: Mountrakker] 1
#12248577 - 03/22/10 12:21 PM (13 years, 10 months ago) |
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(wheat berries)i tried one batch so far. i was contaminated by my LC. but my buddy says there just like rye berries...so im going up to the coop today to get more and try again tonight. i have tons of rye berries all 100% colonized i can sho u a pic.. and rye and wheat berries doing GRAIN TO GRAIN colonizes pretty dam fast in the jars... also yes i made A NEW LC three days ago and its already filling up with myc. i stir em like ten times a day so thell colonize faster
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Afoafs Friend
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Is a ventilation hole absolutely required? Has anyone tried this Tek without one? It seems that the absence of a ventilation hole would decrease the chance of contamination and ease in shaking the jar. I apologize if this is a stupid question.
-------------------- "I am warning you with peace and love."
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santaclausmush
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no ventilation hole needed. u can do lc in baby bottles with no ventilation hole.
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Estario
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Without a ventilation hole, when you suck up LC the vacuum you create inside the vessel will draw in unfiltered air in the absence of a filter.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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santaclausmush
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#12376454 - 04/12/10 05:59 AM (13 years, 9 months ago) |
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how are u gonna draw it outta the jar buy putting a hole with scotts tape over it? or are u using a baby bottle? if so take a extra needle pull plunder out and put polyfill in plunger then into jar itll allow air in the jar so u dont create a vacuum./
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Cloneufc
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This is a complicated way to make an LC. All you need is about two teaspoons of honey per pint and a filter. Add honey, water and PC, its that simple. There's also no need to shake an LC. I use micropore tape and swirl my LC's to break up the mycelium, all while keeping my filter dry. Sediment in an LC does not matter. The mycelium eventually breaks down the sediment anyways. Clear LC's can be contaminated, you can't tell by looking at it. You really dont know if an LC is good or not until you use it. Thats why some people on here dont recommend them.
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sicis
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Re: Honey LC Tek - pictorial [Re: Cloneufc] 1
#13496748 - 11/17/10 06:56 AM (13 years, 2 months ago) |
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*
Edited by sicis (02/19/13 11:36 AM)
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Doc_T
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Re: Honey LC Tek - pictorial [Re: sicis] 1
#13496774 - 11/17/10 07:10 AM (13 years, 2 months ago) |
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Hi, you need to learn PF Tek. You can start your PF jars with LC or spores. But you have to grow cakes, it doesn't go LC->shrooms.
-------------------- You make it all possible. Doesn't it feel good?
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sicis
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Re: Honey LC Tek - pictorial [Re: Doc_T] 1
#13496787 - 11/17/10 07:14 AM (13 years, 2 months ago) |
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The problem is that i dont have Vermiculite. I got Brown rice and honey. I would make LC and infect it in BRF jars, wait for myc than break it up and case them in plastic box then put them in incubator. I also have no access to Vermiculite. I planned to go to capital city to buy it, but the seller has run out of vermiculite and dont know when it will be in stock, so i dont want to wait cuz i want to trip out in winter. First experience
Edited by sicis (11/17/10 07:17 AM)
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Doc_T
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Re: Honey LC Tek - pictorial [Re: sicis] 1
#13496789 - 11/17/10 07:15 AM (13 years, 2 months ago) |
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That won't work. Go get verm, do it right.
-------------------- You make it all possible. Doesn't it feel good?
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sicis
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Re: Honey LC Tek - pictorial [Re: Doc_T] 1
#13496800 - 11/17/10 07:19 AM (13 years, 2 months ago) |
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Why verm wtf why always its vermiculite i mean in nature it grows like on wood etc, theres no vermiculite in nature. Is there any other alternative beside coir cow horse manures? Cuz i have no access to them also i can get perlite though, but i read not to use it.
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Doc_T
Random Dude




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Re: Honey LC Tek - pictorial [Re: sicis] 1
#13496820 - 11/17/10 07:25 AM (13 years, 2 months ago) |
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What part of the world do you live in that there's no verm? Forget about Wal-Mart dude, they don't have what you need.
look, you can grow mushrooms if you follow the tek. If you don't follow the tek, then you may or may not grow mushrooms. Mostly 'not'.
-------------------- You make it all possible. Doesn't it feel good?
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sicis
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Re: Honey LC Tek - pictorial [Re: Doc_T] 1
#13496828 - 11/17/10 07:28 AM (13 years, 2 months ago) |
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im from Latvia, and to find Verm i must go to capital city Riga and theres a chance that i wont find it and it would be big waste. The source from where i planned to get verm has run out of it.
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Doc_T
Random Dude




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Re: Honey LC Tek - pictorial [Re: sicis] 1
#13496838 - 11/17/10 07:32 AM (13 years, 2 months ago) |
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Oh, Latvia? You should have said so. I think you'd be better off with one of the horse manure/straw methods. But verm is very light, it would not be too bad to mail it.
Do you have a pressure cooker? It's not required, but opens up options.
-------------------- You make it all possible. Doesn't it feel good?
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sicis
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Re: Honey LC Tek - pictorial [Re: Doc_T] 1
#13496851 - 11/17/10 07:36 AM (13 years, 2 months ago) |
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No i dont have pressure cooker but im ok to steam it for 2h The mailing would be more pricey than hitchiking to Riga and getting back by bus. As i was planning to do cuz i also need biogrow for my weed
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Doc_T
Random Dude




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Re: Honey LC Tek - pictorial [Re: sicis] 1
#13496858 - 11/17/10 07:38 AM (13 years, 2 months ago) |
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Find a pressure cooker somewhere. It will make everything else much simpler. But you can probably grow without one if you can get vermiculite. There's really no substitute, it's a unique product.
-------------------- You make it all possible. Doesn't it feel good?
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sicis
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Re: Honey LC Tek - pictorial [Re: Doc_T] 1
#13496879 - 11/17/10 07:44 AM (13 years, 2 months ago) |
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How long spores are viable in syringe? Cuz i want to do it right, and the straw tek seems bit complicated, i understood the process of PF thought.
Edited by sicis (11/17/10 07:45 AM)
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Doc_T
Random Dude




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Re: Honey LC Tek - pictorial [Re: sicis] 3
#13496891 - 11/17/10 07:50 AM (13 years, 2 months ago) |
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Spores will last quite some time, months at least. All methods are about equally complicated. Once you understand the process, it's not that hard. (Think of cooking, for example.)
The most important issue is what supplies and equipment you have. Find a tek you can follow with what's available to you, that will work better than making your own the first time.
When you decide, check back here and we can help you with specific questions or problems.
-------------------- You make it all possible. Doesn't it feel good?
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STIKAROUND
Rio



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Re: Honey LC Tek - pictorial [Re: Estario] 1
#13798280 - 01/16/11 06:13 PM (13 years, 14 days ago) |
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Could I possibly cut the measurements for everything in half? 250ml jar (1/2 pint), 175ml distilled water w/ 7ml of organic honey, and use a 10cc syringe?
-------------------- The s GAIA
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Base Icks



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Re: Honey LC Tek - pictorial [Re: STIKAROUND] 1
#13799402 - 01/16/11 09:32 PM (13 years, 13 days ago) |
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Yes, there is nothing wrong with scaling a recipe.
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dreaminglad
jojogunz



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Re: Honey LC Tek - pictorial [Re: Estario] 1
#15006158 - 08/31/11 11:35 AM (12 years, 4 months ago) |
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if your using spore syringe, how much should you inject to start fresh jar ?
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Roman08640
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lawl Mom, get off shroomery and make dinner!
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Greatdyskensia
The



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Haha! Man, that's the worst. Did you notice any issues with he growth, or did the mycelium happily eat the cat keratin? There is cat hair in the ambiant emvironment in my home, so That's definitely an issue in my life.
-------------------- Colaberation, not competition.
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Greatdyskensia
The



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Re: Tap water Vs Mineral Water [Re: Kaoskid] 1
#24794074 - 11/19/17 05:14 PM (6 years, 2 months ago) |
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Where did you get them from that was better? I'm looking for a new reasonable Canadian source
-------------------- Colaberation, not competition.
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bodhisatta 
Smurf real estate agent


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Check thread dates.
This tek is pretty bad tho. Tap water is fine and honey LC is probably one of the worst recipes for LC. As with any simple sugar LC
And you should never inoculate a LC with spores.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Honey LC Tek - pictorial (moved) [Re: Estario] 1
#24794211 - 11/19/17 06:13 PM (6 years, 2 months ago) |
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This thread was moved from Mushroom Cultivation.
Reason: Old school LC tek.
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