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Estario
Enthusiast



Registered: 05/01/08
Posts: 205
Last seen: 6 years, 10 months
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This is a ready LC done the same way, which I am using to inoculate the ready jars.
 This is how a jar should look like. There should be no sedimentations inside. If there are you should do the process again, because from my personal experience jars with caramelized honey are simple not being colonized.
 To draw LC from a jar you need to flame sterilize you needle.
 A syringe that is ready for use:
 About the shaking. I don't shake my jars that hard. Mycelium usually doesn't form so thick clouds with the setup I use and there is no real need. As for the speed of colonization, it is not so important for me as I always keep some LC ready for use. (1 jar in my incubator and 1 backup jar in the fridge) In case it gets contaminated or runs out I prepare some more with the backup LC and use it to inoculate any future projects until the new one is ready. I am doing this how pictorial, because my old LC got contaminated. I find this tek works for me perfectly. There are better solutions, providing more mycelium and faster colonization, such as using magnetic stirrer or potato LC, but I am a big fan of the KIS(keep it simple) idea. If something works well enough don't overtweak it.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Honey LC Tek - pictorial [Re: sicis] 3
#13496891 - 11/17/10 07:50 AM (13 years, 2 months ago) |
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Spores will last quite some time, months at least. All methods are about equally complicated. Once you understand the process, it's not that hard. (Think of cooking, for example.)
The most important issue is what supplies and equipment you have. Find a tek you can follow with what's available to you, that will work better than making your own the first time.
When you decide, check back here and we can help you with specific questions or problems.
-------------------- You make it all possible. Doesn't it feel good?
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Plastered marble
All posts fictional

Registered: 12/28/08
Posts: 216
Last seen: 9 years, 3 months
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Re: Honey LC Tek - pictorial [Re: Estario] 2
#10466646 - 06/07/09 04:25 PM (14 years, 7 months ago) |
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You're mycelium loves to make snowballs allright!
Also: did you know a lot of nail clippers have a little "hook" on the inside to scrape fingernails clean? ... just saying ...
-------------------- I survived operation midnight climax and all I got was really, really high. (older sigs)
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MagicMaker
The Lizard King




Registered: 10/10/06
Posts: 626
Loc: Terrapin
Last seen: 5 years, 9 months
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Re: Early enzyme development in LC [Re: Estario] 2
#10988080 - 09/02/09 05:27 PM (14 years, 4 months ago) |
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good point about the tubing that would seem to protect the filter from getting wet due to swirling, and def easier to replace, more air flow too but i bet just pulling through a hole packs it a lot tighter which would be better to keep out contam, but whatever floats ur boat i can def see wetting the polyfil being an issue
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Mountrakker
r2d2



Registered: 12/28/09
Posts: 200
Loc: Europe
Last seen: 12 years, 2 months
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Hey thanks for the tip, though i have to say that its my Pf-tek Jars that haven't colonized in 20 days, not my Lc that is why i shot them around 3-4 cc honey lc again to test..
it is highly probable that you are right and it is contamed, will know soon for sure though, time will tell as Bob Marley sais

hey the bird seed is really cool, popcorn is ok, rye berries is the last i haven't tried and i guess the best of them all
will keep this process posted once i get any results
tx!
-------------------- Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer
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Estario
Enthusiast



Registered: 05/01/08
Posts: 205
Last seen: 6 years, 10 months
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Honey LC Tek - pictorial 1
#10446142 - 06/03/09 06:21 AM (14 years, 7 months ago) |
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Honey LC Tek I had to make some new LC and decided I would share my method. Honey is a bit tricky to use as it caramelizes pretty easy, but this is the only additive I have had success with. With some caution during heat exposure I get pretty good LC jars without any sedimentation. I have used fructose, but the mycelium grew weak. So here it is. The material you will need: Organic Honey 20 ml syringe Mineral water 500 ml jars something to measure the amount of water used Proper jar lids (Jar lid with silicon injection port and polyfil filter (Tek) )
Step 1 Put some honey in a cup. My honey is very pure and I had to melt it down on a water bat. Don't use microwave as the honey will boil in just few seconds and you will have sedimentation, from crystals forming during the boiling.
 This is what it should look like, when ready for use:

Step 2 Take 11 ml of honey with a syringe.

Step 3 Use mineral water and measure 250 ml of it. Don't use tap water as it might be chemically treated. Tap water is being cleaned out of bacteria and fungi and it has additives to prevent them from contaminating it. You want exactly the opposite. You want fungi mycelium to grow in this water. Also mineral water carries its own energy.

Step 4 Mix the water and the honey in a 500 ml jar. If you use different amounts of water the ratio you are aiming at is 4 ml of honey per 100 ml of water (around 4 % honey solution).
 I warm the jars in a water bat to completely dissolve the honey.

Step 5 Put the lid on. I use lids with an air port and a self healing silicon injection port. They are pretty easy to make and provide real good air exchange as well as very good sealing after inoculation. Soon I will have to make some more and I will post a pictorial. EDIT: Here is the tek. Jar lid with silicon injection port and polyfil filter (Tek)

Step 6 Pressure cook your jars. First ensure they don't touch the bottom or the sides of the pressure cooker as the worm up and too high temperatures cause crystalisation. I use an old metal box to raise the jars above the bottom. Be creative.
 I put one layer of aluminum foil and PC for 15 minutes.
 Every 5 minutes I check the PC to see if it is boiling. When it starts boiling I turn down the heat and measure 15 minutes from that moment.
Edited by Estario (12/28/09 12:24 AM)
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Carcass
Polarized


Registered: 02/15/09
Posts: 407
Last seen: 7 years, 29 days
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10446154 - 06/03/09 06:30 AM (14 years, 7 months ago) |
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Thanks for sharing. Good pictorial.
   
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NoBrainNoPain
Enthusiast noob


Registered: 03/27/09
Posts: 427
Loc: here
Last seen: 8 years, 10 months
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Re: Honey LC Tek - pictorial [Re: Carcass] 1
#10448039 - 06/03/09 02:37 PM (14 years, 7 months ago) |
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How do you prevent your filter from getting wet when you shake your LC?
-------------------- I would love a woodlover's print… PM If you have one and want to trade
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protestsong
City Fox


Registered: 02/28/09
Posts: 242
Loc: north of the 49th
Last seen: 13 years, 7 months
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10448790 - 06/03/09 04:51 PM (14 years, 7 months ago) |
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how did you make your "alcohol lamp"? i'm pretty sure i know how... i just don't want to blow myself up or anything!
-------------------- "Everything you can imagine is real." - Pablo Picasso "Reality is merely an illusion, albeit a very persistent one" - Albert Einstein "He felt that his whole life was some kind of dream and he sometimes wondered whose it was and whether they were enjoying it." - Douglas Adams "Beauty is in the eye of the beholder, and it may be necessary from time to time to give a stupid or misinformed beholder a black eye." - Miss Piggy
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Estario
Enthusiast



Registered: 05/01/08
Posts: 205
Last seen: 6 years, 10 months
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Re: Honey LC Tek - pictorial [Re: protestsong] 1
#10448897 - 06/03/09 05:10 PM (14 years, 7 months ago) |
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You simply drill a hole in the lid and use some cotton wool as a wick. Noting too fancy or complicated. The more wick you have sticking out, the bigger the flame will be.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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Estario
Enthusiast



Registered: 05/01/08
Posts: 205
Last seen: 6 years, 10 months
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10465642 - 06/07/09 12:23 PM (14 years, 7 months ago) |
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And here are the results 4 days later.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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Estario
Enthusiast



Registered: 05/01/08
Posts: 205
Last seen: 6 years, 10 months
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Yep. I noticed the fingernail issue and took care of it. Right now I have exams and I seem to forget to do simple things such as cutting my nails.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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spoonman_
Stranger
Registered: 06/05/09
Posts: 11
Last seen: 14 years, 7 months
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10469341 - 06/08/09 03:11 AM (14 years, 7 months ago) |
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Thanks for this, nice pictures.
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romulux
Connoisseur of Hallucination



Registered: 08/11/08
Posts: 74
Loc: United States
Last seen: 12 years, 4 months
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Re: Honey LC Tek - pictorial [Re: spoonman_] 1
#10469532 - 06/08/09 05:28 AM (14 years, 7 months ago) |
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awesome tek, thanks dude!
-------------------- My psytrance: Soundcloud:Facebook
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Estario
Enthusiast



Registered: 05/01/08
Posts: 205
Last seen: 6 years, 10 months
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Re: Honey LC Tek - pictorial [Re: romulux] 1
#10505561 - 06/14/09 04:39 PM (14 years, 7 months ago) |
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I have a question. Lately I have been reading All Of RR's Notes On Mushroom Cultivation Forum. It is something quite big and full of information. I have changed many aspects of my growing procedures. Recently I got to the part where RR speaks about LC. Apart from recommending against inoculating LC directly with spores he mentions a recipe for LC.(this is the better way of making LC)
Quote:
*Extra light malt, dextrose, nutritional yeast, gypsum. Makes the strongest growth. LC/AGAR
Now I have 3 questions. First one is: Can I use small amount of gypsum and nutritional yeast with honey? Is brewers nutritional yeast the same as the brewers yeast I take as vitamins? Second question. Dextrose is a sugar. I know that. But I am not sure if extra light malt is the same as pure maltose? Can one use coffee as additive to the LC to lower pH and add some nutritions to the mix? The water I use for LC has a pH of around 8,5.
Edited by Estario (06/16/09 09:45 AM)
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misfitdeviate

Registered: 06/20/09
Posts: 762
Loc: ESSR
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Re: Honey LC Tek - pictorial [Re: Estario] 1
#10572394 - 06/25/09 05:27 PM (14 years, 7 months ago) |
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hey, thanks for this tek, I did it for my first time about a week ago and everything has worked out great!
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Estario
Enthusiast



Registered: 05/01/08
Posts: 205
Last seen: 6 years, 10 months
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I ran out of mineral water and I had to prepare 8 jars and PC them in 2 different pressure cookers. They all ended up with caramelized. I don't know what tap water in my area contains, but it definitely doesn't work for me.
-------------------- All Of RR's Notes On Mushroom Cultivation Forum - a must read
Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin.
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talane2005
Enthusiast



Registered: 05/03/09
Posts: 15
Loc: Lawrence
Last seen: 14 years, 1 month
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Re: Tap water Vs Mineral Water [Re: Estario] 1
#10735783 - 07/24/09 10:37 PM (14 years, 6 months ago) |
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very helpful post. had some pretty good growth, however, even with a small amount of sediments. but you're right, most of my jars that did contain the sediments simply didn't colonize.
great clear pictures. hopefully no more "what should finished LC look like" questions.
-------------------- See you later, Space Cowboy...
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Kaoskid
inspiring mycologist


Registered: 07/18/09
Posts: 12
Last seen: 13 years, 10 months
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Re: Tap water Vs Mineral Water [Re: talane2005] 1
#10735929 - 07/24/09 11:07 PM (14 years, 6 months ago) |
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im kinda new here and failed at my attempt to create a LC using karo. im now attempting honey. could you clarify the part when your pressure cooking them. you say you check it every five mins to see if its boiling and then you turn the heat down and pressure cook it for 15 mins. i tried that and my whistle never blew on my PC. so are you actually achieving the 15psi or what ever psi your going for, or are you just kind of "steaming them"?
and my first attempt might of failed due to my syringe. it was from spores101 and after i got some from elsewhere i could see how bad spores101 sucks. i ordered them prior to my research.
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Gnarly Tourettes
Stranger



Registered: 07/22/09
Posts: 60
Last seen: 14 years, 6 months
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Re: Tap water Vs Mineral Water [Re: Kaoskid] 1
#10736197 - 07/24/09 11:57 PM (14 years, 6 months ago) |
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i have an lc colinizing with a cat hair in it.. had to of been there before sterilizing maybe stick to the inside of the jar lid or something..
i just see it floating around in the myc like wtf stupid cat
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