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OfflineMagicMaker
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Re: Early enzyme development in LC [Re: Estario] * 1
    #10987848 - 09/02/09 04:49 PM (14 years, 4 months ago)

Quote:

Estario said:
Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive.
Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.





interesting theory, sounds plausible, i have been doing research as I am going to make my first LC soon, this tek looks like a good one to try, my question is, to get the LC out after colonizing it do u just tip the jar as not to wet the polyfil and suck it up? i guess thats the only way,

also want to say you could probably just pull polyfil through a small hole instead of bothering to insert a tube and have to silicon it on both sides, might save you some time,

thanks for the alcohol lamp though thats a damn good idea, you think a peice of a shirt will work or does it have to be wool?


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OfflineMagicMaker
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Re: Early enzyme development in LC [Re: Estario] * 2
    #10988080 - 09/02/09 05:27 PM (14 years, 4 months ago)

good point about the tubing that would seem to protect the filter from getting wet due to swirling, and def easier to replace, more air flow too but i bet just pulling through a hole packs it a lot tighter which would be better to keep out contam, but whatever floats ur boat i can def see wetting the polyfil being an issue


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