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OfflineEstario
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Honey LC Tek - pictorial * 1
    #10446142 - 06/03/09 06:21 AM (14 years, 7 months ago)

Honey LC Tek

I had to make some new LC and decided I would share my method. Honey is a bit tricky to use as it caramelizes pretty easy, but this is the only additive I have had success with. With some caution during heat exposure I get pretty good LC jars without any sedimentation. I have used fructose, but the mycelium grew weak.
So here it is.
The material you will need:
Organic Honey
20 ml syringe
Mineral water
500 ml jars
something to measure the amount of water used
Proper jar lids (Jar lid with silicon injection port and polyfil filter (Tek) )


Step 1
Put some honey in a cup. My honey is very pure and I had to melt it down on a water bat. Don't use microwave as the honey will boil in just few seconds and you will have sedimentation, from crystals forming during the boiling.

This is what it should look like, when ready for use:


Step 2
Take 11 ml of honey with a syringe.


Step 3
Use mineral water and measure 250 ml of it. Don't use tap water as it might be chemically treated. Tap water is being cleaned out of bacteria and fungi and it has additives to prevent them from contaminating it. You want exactly the opposite. You want fungi mycelium to grow in this water. Also mineral water carries its own energy.


Step 4
Mix the water and the honey in a 500 ml jar. If you use different amounts of water the ratio you are aiming at is 4 ml of honey per 100 ml of water (around 4 % honey solution).

I warm the jars in a water bat to completely dissolve the honey.


Step 5
Put the lid on. I use lids with an air port and a self healing silicon injection port. They are pretty easy to make and provide real good air exchange as well as very good sealing after inoculation. Soon I will have to make some more and I will post a pictorial.
EDIT: Here is the tek.
Jar lid with silicon injection port and polyfil filter (Tek)


Step 6
Pressure cook your jars. First ensure they don't touch the bottom or the sides of the pressure cooker as the worm up and too high temperatures cause crystalisation. I use an old metal box to raise the jars above the bottom. Be creative.

I put one layer of aluminum foil and PC for 15 minutes.

Every 5 minutes I check the PC to see if it is boiling. When it starts boiling I turn down the heat and measure 15 minutes from that moment.


Edited by Estario (12/28/09 12:24 AM)


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OfflineEstario
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Re: Honey LC Tek - pictorial [Re: NoBrainNoPain] * 3
    #10448657 - 06/03/09 04:30 PM (14 years, 7 months ago)

This is a ready LC done the same way, which I am using to inoculate the ready jars.

This is how a jar should look like. There should be no sedimentations inside. If there are you should do the process again, because from my personal experience jars with caramelized honey are simple not being colonized.

To draw LC from a jar you need to flame sterilize you needle.

A syringe that is ready for use:

About the shaking. I don't shake my jars that hard. Mycelium usually doesn't form so thick clouds with the setup I use and there is no real need. As for the speed of colonization, it is not so important for me as I always keep some LC ready for use. (1 jar in my incubator and 1 backup jar in the fridge) In case it gets contaminated or runs out I prepare some more with the backup LC and use it to inoculate any future projects until the new one is ready. I am doing this how pictorial, because my old LC got contaminated. I find this tek works for me perfectly. There are better solutions, providing more mycelium and faster colonization, such as using magnetic stirrer or potato LC, but I am a big fan of the KIS(keep it simple) idea. If something works well enough don't overtweak it.


--------------------
All Of RR's Notes On Mushroom Cultivation Forum - a must read
:alert: Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin. :alert:


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OfflineEstario
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Re: Honey LC Tek - pictorial [Re: Estario] * 1
    #10465642 - 06/07/09 12:23 PM (14 years, 7 months ago)

And here are the results 4 days later.


--------------------
All Of RR's Notes On Mushroom Cultivation Forum - a must read
:alert: Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin. :alert:


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Invisiblemisfitdeviate

Registered: 06/20/09
Posts: 762
Loc: ESSR
Re: Honey LC Tek - pictorial [Re: Estario] * 1
    #10572394 - 06/25/09 05:27 PM (14 years, 7 months ago)

hey, thanks for this tek, I did it for my first time about a week ago and everything has worked out great!



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OfflineEstario
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Early enzyme development in LC [Re: Gnarly Tourettes] * 1
    #10985649 - 09/02/09 10:32 AM (14 years, 4 months ago)

Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive.
Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.


--------------------
All Of RR's Notes On Mushroom Cultivation Forum - a must read
:alert: Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin. :alert:


Edited by Estario (09/02/09 10:33 AM)


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Invisiblecacharstar
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Re: Early enzyme development in LC [Re: Estario] * 1
    #11603541 - 12/07/09 01:46 PM (14 years, 1 month ago)

Quote:

Estario said:
Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive.
Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.




Would this improve the acceptance time of lc to wood lovers substrate as well? 
Also if a culture is taken from fruiting bodies or transfer from agar to agar from the wild, then the DNA should be unlocked right? This applies to germinated spores I assume?:shrug:


--------------------
:irishtoast:


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OfflineEstario
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Re: Early enzyme development in LC [Re: cacharstar] * 1
    #11729409 - 12/28/09 12:37 AM (14 years, 1 month ago)

Quote:

cacharstar said:
Quote:

Estario said:
Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive.
Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.




Would this improve the acceptance time of lc to wood lovers substrate as well? 
Also if a culture is taken from fruiting bodies or transfer from agar to agar from the wild, then the DNA should be unlocked right? This applies to germinated spores I assume?:shrug:



Paul Stamets explains this topic pretty extensively in his book Growing Gourmet and Medicinal Mushrooms. He recommends using the soak water from grains to make LC. I have stored some but right now I don't have the need for any more LC so I will use it later. If any one is interested in this topic read page 107.
Quote:

To ameliorate degenerative effects, the
addition of extracted end-substrates (sawdust,
straw, etc.) favors the normal development of
mycehium. The introduction of the end-sub-
strate acquaints the mushroom mycelium with
its destined fruiting habitat, challenging the
mycelium and selectively activating its enzy-
matic systems. This familiarity with the
end-substrate greatly improves performance
later on. Parent cells retain a"genetic memory"
passed downstream through the mycelial net-
works.
Paul Stamets - Growing Gourmet and Medicinal Mushrooms




--------------------
All Of RR's Notes On Mushroom Cultivation Forum - a must read
:alert: Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin. :alert:


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Offlinesantaclausmush
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Registered: 02/25/08
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Re: Honey LC Tek - pictorial [Re: Estario] * 1
    #12217723 - 03/17/10 10:01 AM (13 years, 10 months ago)

Quote:

Estario said:
And here are the results 4 days later.



in this tek did ur mycelium stay and grow at the bottom of the jar? i have six bottles already growing myc. in all of em. but they are all contammed. but they still grow bigger clouds everyday.


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Offlinekurshiukas
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Re: Honey LC Tek - pictorial [Re: santaclausmush] * 1
    #12217963 - 03/17/10 11:01 AM (13 years, 10 months ago)

Quote:

santaclausmush said:
Quote:

Estario said:
And here are the results 4 days later.



in this tek did ur mycelium stay and grow at the bottom of the jar? i have six bottles already growing myc. in all of em. but they are all contammed. but they still grow bigger clouds everyday.




if by contam you mean mold, then the clouds you see are probably mold clouds. Mold myc is the white as shroom myc as long as it does not sporrate.


--------------------
AMU


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Offlinesantaclausmush
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Re: Honey LC Tek - pictorial [Re: kurshiukas] * 1
    #12218053 - 03/17/10 11:14 AM (13 years, 10 months ago)

this is my clouds. there white but im colorblind so i put em in front of my high pressure sodium grow light to see and there white. but i got contam on all of it. this is all good. some i have black dots in and they all are contamed but huge myc clouds it loooks like.


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OfflineMountrakker
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Re: Honey LC Tek - pictorial [Re: Estario] * 1
    #12230860 - 03/19/10 12:35 PM (13 years, 10 months ago)

hey thanks for this great tek, the lids look really prof now

i have done 2 Honey Lc jars by the book/tek but there was too much sediment according to my judgement, is there any chance the flowing around sediment would use as an anchorage for the mycelium? they haven't formed a dense blob as of yet, and they've been in there for more than a week


i like the right jar more, it looks better, and it looks as if it has too types of sediment, the brown (caramelized) and tiny bits and clumps that have formed white hairs on them.
The left one (short one) had too much sediment to begin with (more bio-honey) and i guess it will be discarded

what do you guys think?

now i've got bird seed and popcorn waiting to be pressure cooked and sterilized, as to test the formations in the honey Lc, do you guys think ill have any chance of success? im really new to Lc and in my country(europe) we haven't got Karo products although today at the supermarket i saw some artificial sweetener sugar with a malt-dextrose mix..frustrating..:confused:

peace out


--------------------
Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer


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OfflineMountrakker
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Re: Early enzyme development in LC [Re: Estario] * 1
    #12235563 - 03/20/10 06:03 AM (13 years, 10 months ago)

Okay, so the Honey Lc has too much sediment and i'm overconfident with what i do, i guess those two jars in my previous thread have nothing but sediment, and the spores can't get a grip

question : Sedimentation retarded mycelium from forming. The suspended spores should be still vital right?

in this pic i want to make Lc to Lc tranfer, havent done that before


the left one is the one with sediment + spores
the right one is 4% honey properly done

has anybody had success with that? remember i want to do Lc to Lc but with no clear mycleium formation from the first, just the suspended spores (or clumps)

thanks


--------------------
Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer


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Offlinesantaclausmush
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Re: Early enzyme development in LC [Re: Mountrakker] * 1
    #12235584 - 03/20/10 06:16 AM (13 years, 10 months ago)

Quote:

Mountrakker said:
Okay, so the Honey Lc has too much sediment and i'm overconfident with what i do, i guess those two jars in my previous thread have nothing but sediment, and the spores can't get a grip

question : Sedimentation retarded mycelium from forming. The suspended spores should be still vital right?

in this pic i want to make Lc to Lc tranfer, havent done that before


the left one is the one with sediment + spores
the right one is 4% honey properly done

has anybody had success with that? remember i want to do Lc to Lc but with no clear mycleium formation from the first, just the suspended spores (or clumps)

thanks


  both jars if u inoculate them will grow a fluffy sediment in it... did u use distilled water... i like using distilled water when i make LC cause theres nothing in the water and it always looks clearer. so u can see the myc better.. but test the LC on one jar first before u go making all these jars then inoculate them and they all grew contaminated.this LC JAR is contaminated...  those other pics are the white fluffy stuff in em but when i inoculated my stuff it was all contam...you get fluffy white stuff if its contammed or not... so b carefull so u dont waste all that time..
THE CLEARER HONEY U CAN FIND THE BETTER.USING DISTILLED WATER IS BETTER.
ive tried 3 kinds of honey and all worked...
just use a test jar to make sure its good...

ne thing elsee i can help u with..he liquid in this jar i used to make LC with cause the liquid and the myc are 100% colonized so i sucked some liquid out of the jar and used it to make LC.  then i took the LC and inoculated one jar with brf and it turned white white so no contam in that LC... make sure u dont contam ur LC JAR before, after or during inoculation.. then u wont know its contamed and all ur jars will be contamed.


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Offlinesantaclausmush
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Re: Early enzyme development in LC [Re: Mountrakker] * 1
    #12248577 - 03/22/10 12:21 PM (13 years, 10 months ago)

(wheat berries)i tried one batch so far. i was contaminated by my LC. but my buddy says there just like rye berries...so im going up to the coop today to get more and try again tonight. i have tons of rye berries all 100% colonized i can sho u a pic.. and rye and wheat berries doing GRAIN TO GRAIN colonizes pretty dam fast in the jars...    also yes i made  A NEW LC three days ago and its already filling up with myc. i stir em like ten times a day so thell colonize faster


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