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 dankcrop
unknown
Registered: 09/30/02
Posts: 149
Last seen: 9 years, 1 month
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 Slowww Growth 
#1044034 - 11/11/02 05:58 PM (15 years, 5 months ago) |
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Its been exactly 14 days since innoculation and only 3 out of 10 jars have started. They have about 25% colonization. the other 7 show 0 signs. I dont know what to do. Temps are constant at about mid 60's to 70. maybe they need more time..i dunno
btw the 3 jars that have colonzation, had about 1cc more of solution than that of the other 7, b/c i had some left over in the syringe at the end. should i just chuck the 7 jars or do they still have a chance? theres no mold or anything growing in them, they look the same as when i prepared them 2 weeks ago.
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StS
treetoker
 
Registered: 08/01/00
Posts: 1,821
Loc: Earth
Last seen: 10 months, 24 days
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Re: Slowww Growth [Re: dankcrop]
#1044040 - 11/11/02 06:01 PM (15 years, 5 months ago) |
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Raise the temps to around 80-85. The ones with more spores would have a chance of germinating faster. Don't chuck your jars yet, just raise the temps and wait longer!
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"You took too much, man....too much, too much!"
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Llamanose
The llama knows
Registered: 10/02/02
Posts: 1,868
Last seen: 2 years, 6 months
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Re: Slowww Growth [Re: dankcrop]
#1044050 - 11/11/02 06:04 PM (15 years, 5 months ago) |
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They need to be warmer, yo! 80-85 or so if you can raise the temp, it will speed things immensely. 
As for the ones that aren't movin... did you shake they syringe before and in between innocs? Sterilize everything? Warm things up and wait another week or two... Patience, grasshopper.
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Alice came to a fork in the road. "Which road do I take?" she asked.
"Where do you want to go?" responded the Cheshire cat.
"I don't know," Alice answered.
"Then," said the cat, "it doesn't matter."
~Lewis Carroll, Alice in Wonderland
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dankcrop
unknown
Registered: 09/30/02
Posts: 149
Last seen: 9 years, 1 month
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Re: Slowww Growth [Re: dankcrop]
#1044285 - 11/11/02 08:02 PM (15 years, 5 months ago) |
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No I didnt shake in between innocs ;( I knew i forgot something. But i did shake the syringe good before the start. The whole process took me 30 mintues. I did sterilize with an alch flame and then I wiped the needle with alco every 2 jars.Then put tape over holes. I removed the tape a week ago. I Dont know if that makes a difference. I was even thinking about using another syringe for the 8 jars with delayed signs of growth.
Thanks for the feedback and I think i need to invest in an incubator.
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