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Offlinedankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 7 years, 8 months
Slowww Growth
    #1044034 - 11/11/02 05:58 PM (14 years, 24 days ago)

Its been exactly 14 days since innoculation and only 3 out of 10 jars have started. They have about 25% colonization. the other 7 show 0 signs. I dont know what to do. Temps are constant at about mid 60's to 70. maybe they need more time..i dunno

btw the 3 jars that have colonzation, had about 1cc more of solution than that of the other 7, b/c i had some left over in the syringe at the end. should i just chuck the 7 jars or do they still have a chance? theres no mold or anything growing in them, they look the same as when i prepared them 2 weeks ago.


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OfflineStS
treetoker
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Registered: 08/01/00
Posts: 1,821
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Re: Slowww Growth [Re: dankcrop]
    #1044040 - 11/11/02 06:01 PM (14 years, 24 days ago)

Raise the temps to around 80-85. The ones with more spores would have a chance of germinating faster. Don't chuck your jars yet, just raise the temps and wait longer!


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"You took too much, man....too much, too much!"


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OfflineLlamanose
The llama knows

Registered: 10/02/02
Posts: 1,868
Last seen: 1 year, 1 month
Re: Slowww Growth [Re: dankcrop]
    #1044050 - 11/11/02 06:04 PM (14 years, 24 days ago)

They need to be warmer, yo!  80-85 or so if you can raise the temp, it will speed things immensely.  :grin:

As for the ones that aren't movin...  did you shake they syringe before and in between innocs? Sterilize everything?  Warm things up and wait another week or two...  Patience, grasshopper.  :grin: 


--------------------
Alice came to a fork in the road.  "Which road do I take?" she asked.
"Where do you want to go?" responded the Cheshire cat.
"I don't know," Alice answered.
"Then," said the cat, "it doesn't matter."
~Lewis Carroll, Alice in Wonderland


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Offlinedankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 7 years, 8 months
Re: Slowww Growth [Re: dankcrop]
    #1044285 - 11/11/02 08:02 PM (14 years, 24 days ago)

No I didnt shake in between innocs ;( I knew i forgot something. But i did shake the syringe good before the start. The whole process took me 30 mintues. I did sterilize with an alch flame and then I wiped the needle with alco every 2 jars.Then put tape over holes. I removed the tape a week ago. I Dont know if that makes a difference. I was even thinking about using another syringe for the 8 jars with delayed signs of growth.

Thanks for the feedback and I think i need to invest in an incubator.


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