Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineAkira4321
HNIC
Male User Gallery


Registered: 03/13/02
Posts: 246
Loc: U.S.
Last seen: 5 years, 6 months
Inoculation Of Petri Dish Before Agar Solidification
    #10440303 - 06/02/09 09:12 AM (15 years, 7 months ago)

I finally pour my first agar plates day before yesterday. I think I may have made a mistake though. I only hope that their not doomed. I used PDA which was sterilized in a qt mason jar for forty minutes. I then poured my plates in my glove box. The agar had cooled but only semi started to solidify when I inoculated them with a few drops of solution from a spore syringe. Since I didn't wait for the agar to completely solidify before inoculated did I just botch the whole culturing process or will the spores still germinate?


--------------------
Product Of Genetic Darkness

Extras: Filter Print Post Top
Offlinenoggin
jiggly
Male User Gallery


Registered: 02/17/09
Posts: 228
Loc: South Africa
Last seen: 15 years, 2 months
Re: Inoculation Of Petri Dish Before Agar Solidification [Re: Akira4321]
    #10440317 - 06/02/09 09:14 AM (15 years, 7 months ago)

Should be fine imo.

Extras: Filter Print Post Top
Offlineozzysmygod
Late Night PC'ing Enthusiast
 User Gallery


Registered: 11/26/08
Posts: 835
Last seen: 10 years, 2 months
Re: Inoculation Of Petri Dish Before Agar Solidification [Re: noggin]
    #10440438 - 06/02/09 09:50 AM (15 years, 7 months ago)

If the temperature of the agar was too high, the spores may no longer work. If you see no growth, you should be fine to just drop some more spores on.

Extras: Filter Print Post Top
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: Inoculation Of Petri Dish Before Agar Solidification [Re: Akira4321]
    #18431899 - 06/17/13 01:02 PM (11 years, 6 months ago)

Quote:

Akira4321 said:
I finally pour my first agar plates day before yesterday. I think I may have made a mistake though. I only hope that their not doomed. I used PDA which was sterilized in a qt mason jar for forty minutes. I then poured my plates in my glove box. The agar had cooled but only semi started to solidify when I inoculated them with a few drops of solution from a spore syringe. Since I didn't wait for the agar to completely solidify before inoculated did I just botch the whole culturing process or will the spores still germinate?




id also think if the agar solidified around the spores, they will get no oxygen. if it was solid enough to have them remain on top >.> should be fine unless temps were too high. im no expert, actually only just about to start agar, but im the type of guy the likes to do my research, learn all the ins and outs before I get er done, and do it the right way.


--------------------

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Myyco.com Golden Teacher Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,721 8 02/14/05 07:57 AM
by Roadkill
* Re: Petri Dishes & Agar Q's Placebo 4,118 5 12/07/99 10:00 AM
by Anonymous
* Handling parafilmed inoculated petri dishes... Alegalize 1,695 4 02/26/05 09:11 AM
by scatmanrav
* Pour agar into petri dishes before pc angelko 7,162 5 05/18/03 05:05 PM
by Prisoner#1
* Whats with petri dishes??? Legoulash 1,814 11 01/29/03 04:19 AM
by debianlinux
* pinheads on petri dish Senovio 1,783 7 07/27/03 04:53 PM
by Sev
* premade petri dishes? High_On_Magic 1,984 4 09/15/03 04:10 PM
by simplemachine
* Some of my first petri dishes ever :) kykeon 3,246 7 10/26/02 12:24 AM
by kykeon

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
775 topic views. 14 members, 63 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.015 seconds spending 0.004 seconds on 12 queries.