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Offlinesnapfizzle92
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Registered: 04/07/09
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what happens if i inject inside of the substrate? may have accidentally
    #10367061 - 05/19/09 10:18 PM (14 years, 9 months ago)

looks like i mightve accidentally done it for a few of my jars cause theres small signs of colonization on the outside and its been 2 weeks but for some reason the substrate looks like it could be white on the inside because it appears lighter. is this the reason they arent showing colonization yet? idkkk??

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Offlinejohnwayne
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Registered: 02/19/09
Posts: 548
Loc: 8200 ft
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Re: what happens if i inject inside of the substrate? may have accidentally [Re: snapfizzle92]
    #10367082 - 05/19/09 10:22 PM (14 years, 9 months ago)

how big are your jars? they'll come, sounds like they are colonizing.....also i see no reason injecting the spore solution towards the glass or center will affect growth, since neither application changes the substrate.

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Offlineveda_sticks
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Re: what happens if i inject inside of the substrate? may have accidentally [Re: johnwayne]
    #10367108 - 05/19/09 10:25 PM (14 years, 9 months ago)

Injecting at the edge is recomended so that you see growth as soon as it starts, if something is contaminated you see it right away, rather than it colinise its way through the substrate then appear much later. If you inject into the substrate you just see it much later. It wont effect colinisation time. You just sit waiting longer to see whats happening.


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OfflineScukels
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Registered: 03/22/09
Posts: 145
Last seen: 3 years, 11 months
Re: what happens if i inject inside of the substrate? may have accidentally [Re: veda_sticks]
    #10367332 - 05/19/09 11:23 PM (14 years, 9 months ago)

I like to make my first inoculation site near the glass, for the above reason of watching for contams, and then I make a second inoculation site inside the cake. I do that because I like to think the myce. grows faster in all directions, instead of being limited by the glass barrier. I don't know if my logic makes sense or not.

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