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OfflineRichShroom
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Cloning from Mycelium - a question about Genetics
    #10347842 - 05/16/09 09:04 AM (14 years, 9 months ago)

A question about the exact usage of the word clone from reading this Tek:
Uncle Lazlo's EZ Mycelium Syringe Tek

Quote:

It details a simple and easy method used to clone mushrooms via mycelium syringes.

Here's a way to get into cloning <snip> and allows you to select strong mycelial growth for cloning. <snip>

This technique makes use of a piece of colonized substrate from an unopened, uncontaminated PF-style jar. You will remove a small piece of substrate, place it in a jar of sterile water, agitate the water to distribute mycelial cells through it, and draw a syringe from that jar.




My question is:

* Is the mycelial growth in a jar all one multicelled organism ?

  OR expressed another way:

* Does the growth all contain one gene pattern ?

I grasp the concept of cloning from a mushroom fruit by extracting a piece of core tissue with a syringe - as far as I understand you would then be 'exactly duplicating' that fruit and its genetics.

But does extracting a piece of colonised substrate select a single DNA gene set for replication ?

Or is the word cloning used here in a looser sense.

If I can clarify the difference for myself, it will aid my deeper understanding of the gene-growth-replication cycle, so I would be very happy if someone could explain.

In the broader sense, I'm trying to understand if the colonisation in a PF-style jar is essentially one dividing -> replicating -> expanding organism, or 'a little forest of individuals' each resulting from an individual spore that managed to get a successful foothold once introduced to the substrate ?

If anyone can recommend a good reference on the whole gene - combination - reproduction process, please post a link and I'm more than happy to read up.

Thanks in advance.


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* Hawaiin pins
* Amazonian knots
* Panaeolus cambodginiensis colonising

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OfflineRogerRabbitM
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Re: Cloning from Mycelium - a question about Genetics [Re: RichShroom]
    #10347868 - 05/16/09 09:17 AM (14 years, 9 months ago)

If the jar was inoculated from a multispore syringe or print, probably not.  Thousands of strains can form from a multispore grow.  Most will join into a common organism via the process of anastomosis, but a few will not.  If you want a pure, singe sector isolate, you'll have to learn agar work.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineRichShroom
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Registered: 05/13/09
Posts: 50
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Re: Cloning from Mycelium - a question about Genetics [Re: RogerRabbit]
    #10348808 - 05/16/09 02:04 PM (14 years, 9 months ago)

Anastomosis : Mycology
http://en.wikipedia.org/wiki/Anastomosis

Quote:

In mycology, anastomosis is the fusion between branches of the same or different hyphae.[3] Hence the bifurcating fungal hyphae can form true reticulating networks. By sharing materials in the form of dissolved ions, hormones, and nucleotides, the fungus maintains bidirectional communication with itself. The fungal network might begin from several origins; several spores, several points of penetration, each a spreading circumference of absorption and assimilation. Once encountering the tip of another expanding, exploring self, the tips press against each other in pheromonal recognition, fusing to form a genetic singular that can cover hectares called a genet.

For fungi, anastomosis is also sex. In some fungi, two different haploid mating types - if compatible - merge. Somatically, they form a morphologically similar mycelial wave front that continues to grow and explore. The significant difference, is that in each septated unit is binucleate, containing two unfused nuclei, i.e. one from each parent that do not undergo karyogamy.




Thanks RR ... you've set me off on a path of learning.


--------------------
* B+ fruited & consumed
* Hawaiin pins
* Amazonian knots
* Panaeolus cambodginiensis colonising

Most people do not really want freedom because freedom involves responsibility and most people are frightened of responsibility.

Extras: Filter Print Post Top
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