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OfflineGSAfarmer
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Registered: 06/21/02
Posts: 338
Loc: Mycotopia
Last seen: 19 years, 2 months
Dowl Preperation
    #1032519 - 11/07/02 01:03 PM (21 years, 4 months ago)

This post is for people who would like to share their dowl preperation teks, for people who don't know how or would like to know for future projects. Thanks..........peace


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GSAfarmer Joshua

"Don't live someone elses dream."..........GSAfarmer

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Offliner05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
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Re: Dowl Preperation [Re: GSAfarmer]
    #1033151 - 11/07/02 03:44 PM (21 years, 4 months ago)

What I have done, and has seem to work the best is to use autoclavable filter patch bags with a mix of autoclaved saw dust and the dowel rods. I inoculate that with rye grain. The oysters colonize fast as does ganoderma. Grifola frondosa is slower, and Shitake in between. I have also inoculated straight dowels and I liked the sawdust - dowel mix better. Usually, the dowels are twice as long as you need so I soak mine before autoclaving and cut them in half.

So far I have had the oysters fruiting but the weather has been cold so not alot of growth, but bunch primordia! It is getting warm this weekend so I think things will pop soon. Slugs and squirels like to eat / molest your fruit bodies. Fuckers.


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OfflineGSAfarmer
Mycotopian
Registered: 06/21/02
Posts: 338
Loc: Mycotopia
Last seen: 19 years, 2 months
Re: Dowl Preperation [Re: r05c03]
    #1033402 - 11/07/02 05:40 PM (21 years, 4 months ago)

Thaks for sharing. So after soaking do you add any more water with the bags when you PC the dowls? Also at what PSI and how long do you PC it?..........peace


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GSAfarmer Joshua

"Don't live someone elses dream."..........GSAfarmer

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Offliner05c03
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Re: Dowl Preperation [Re: GSAfarmer]
    #1033424 - 11/07/02 05:49 PM (21 years, 4 months ago)

If I am doing just dowels than I do not use anymore water. If I use a sawdust-dowel mix I weigh the sawdust out in the bags (taking a tare for the bags) and I add water to about 65% by weight, then I add the soaked dowels, PC at 16 psi for 1 hour.


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Offlinezeronio
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Re: Dowl Preperation [Re: GSAfarmer]
    #1034718 - 11/08/02 12:49 AM (21 years, 4 months ago)

I tried this a couple of times. I noticed that wood dowels that were soaked for a week colonized much faster then the ones that were soaked for a day or two. I pressure cooked them in polypropylene bags with tyvek filter patch for 3h and inoculated with grain spawn.
I didn't add water in the bags.

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OfflineAnnoA
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Re: Dowel Preparation [Re: zeronio]
    #1034725 - 11/08/02 12:51 AM (21 years, 4 months ago)

I soak mine in hot water for 1 hour. than they sink to the bottom and don?t take up any more water.

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Offliner05c03
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Re: Dowel Preparation [Re: Anno]
    #1035510 - 11/08/02 08:45 AM (21 years, 4 months ago)

Yeah, yeah, Anno is right. Heating the dowels does help them take up water.


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OfflineSuntzu
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Re: Dowl Preperation [Re: GSAfarmer]
    #1037097 - 11/08/02 04:56 PM (21 years, 4 months ago)

This might not work as well if you do big batches, but for the number of dowels required for a few big logs in the yard this is more than sufficient:

http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum13&Number=977013&page=1&view=collapsed&sb=5&o=&fpart=1

No pressure cooking, just a good hot soak or two. . .has worked for shiitake and reishi without a hitch so far. The others' methods are certainly better and more sure-fire, especially when doing bags of dowels; but this is pretty nice for ease and speed.

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Offlinezeronio
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Re: Dowl Preperation [Re: Suntzu]
    #1042375 - 11/11/02 01:29 AM (21 years, 4 months ago)

That's interesting. I seems that dowels are hard to contaminate.
About longer soaking time... I was soaking dowels and I prepared a part of them after one day and had no time to use the rest. When I inoculated them after one week of soaking they seemed to colonize much better and faster then the first. I will make more trials...

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Offlineviscid
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Registered: 09/23/02
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Re: Dowl Preperation [Re: GSAfarmer]
    #1066576 - 11/19/02 02:51 PM (21 years, 4 months ago)

so hot water definitely helps water uptake.
how bout boiling the dowels as partial sterilization AND to achieve proper moisture?
anyone ever just boiled them?
:laugh: 


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OfflineSuntzu
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Re: Dowl Preperation [Re: viscid]
    #1066906 - 11/19/02 04:27 PM (21 years, 4 months ago)

My impression is that water uptake isn't too tough. . .it can be as simple as letting them sit in room temperature water. . .*long enough*. After they sink, not much more H2O uptake will occur. The other thing that plays a role in dowel success is the actual container that they are incubated in. If it's too big/too spacious, the dowels can dry out during colonization.
Just boiling them would work, I'm sure. . .steeping in hot water appears to be quite sufficient as well.
Something I learned over the past month or so is that dowels are very tough to contaminate.

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Offlineviscid
dikaryon
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Registered: 09/23/02
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Re: Dowl Preperation [Re: Suntzu]
    #1070389 - 11/20/02 02:27 PM (21 years, 4 months ago)

Quote:

dowels are very tough to contaminate.



thats what i'm talkin bout.  word up to that. :grin: 


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Offliner05c03
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Registered: 01/06/02
Posts: 383
Loc: Indiana, US
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Re: Dowl Preperation [Re: GSAfarmer]
    #1070868 - 11/20/02 04:14 PM (21 years, 4 months ago)

I just recieved my 100o dowels from the company that you suggested Suntzu. Thanks for the tip.


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