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OfflineAnnoA
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P. cubensis and P. azurescens jars
    #1034624 - 11/08/02 12:15 AM (21 years, 7 months ago)

I inoculated a couple of Jars prepared according to www.fungifun.org/pf on the 31th of October.

The jar inoculated with P. cubensis Cambodia spores and incubated at 80?F is already nearly completely colonized, the one inoculated with P. azurescens spores and incubated at 70?F is not growing so fast.
I substituted simmered millet for a part of the vermiculte in the recipe, around 20% by volume.


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OfflineExtravagantDream
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1034700 - 11/08/02 12:38 AM (21 years, 7 months ago)

Wow nice grow on that cambodian, that thing is flying. They are one of my favs. Hope your P.azurescen goes better.

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Invisiblezeta
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1034878 - 11/08/02 01:50 AM (21 years, 7 months ago)

What plans do you have for the azurescens cake?

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OfflinePeace_Patrol
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1034886 - 11/08/02 01:53 AM (21 years, 7 months ago)

Hey anno,
Both are lookin pretty good! The azures I would expect to be a lot slower by nature, besides it has only been one week so they are looking great actually! Is 70 F the optimal incubation temperature for azures?

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Offlinebowling-name
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1034912 - 11/08/02 02:01 AM (21 years, 7 months ago)

I would be very interested in seeing you try to fruit the azure cake in a terrarium with moist cardboard draped over it. I've always been skeptical of this "simplified" tek, and would like to see it attempted by someone with experience with this species. While the article is well written, if the methodology is discredited, maybe it should be removed from the growing info section of the shroomery.

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OfflineAnnoA
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Re: P. cubensis and P. azurescens jars [Re: bowling-name]
    #1035250 - 11/08/02 06:43 AM (21 years, 7 months ago)

>What plans do you have for the azurescens cake?
I basically just want to see how long it takes to colonize, and then i want to inoculate some sterilized woodchips with it, the standard route, only that it bypasses agar...

> would be very interested in seeing you try to fruit the azure cake in a terrarium
>with moist cardboard draped over it. I've always been skeptical of
>this "simplified" tek

If you ask me, I think that the species this tek was successful with, was P. cubensis, and not p. azurescens .

>if the methodology is discredited, maybe it should be removed from the growing info section of the shroomery.
Or at least a warning comment added.

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OfflineGrowFast
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1035386 - 11/08/02 07:39 AM (21 years, 7 months ago)

What kind of incubator did you use to incubate P. cubensis Cambodia ??

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Offlinekykeon
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Re: P. cubensis and P. azurescens jars [Re: GrowFast]
    #1035397 - 11/08/02 07:44 AM (21 years, 7 months ago)

a normal one

Kykeon
athens, greece


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OfflineAnnoA
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Re: P. cubensis and P. azurescens jars [Re: GrowFast]
    #1035689 - 11/08/02 10:00 AM (21 years, 7 months ago)

>What kind of incubator did you use to incubate P. cubensis Cambodia ??

Exactly that one:
http://www.fungifun.org/pf/incubator.htm

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InvisibleCLuB99
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1036582 - 11/08/02 02:23 PM (21 years, 7 months ago)

i wonder how you did to produce an azure spore syringe contam free  :confused:

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OfflineAnnoA
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Re: P. cubensis and P. azurescens jars [Re: CLuB99]
    #1037016 - 11/08/02 04:20 PM (21 years, 7 months ago)

>i wonder how you did to produce an azure spore syringe contam free

I?ve choosen a print that looked very "untouched" and healthy and prepared the syringe like I normaly do, using the psilocyber?s syringe tek.

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Invisibletekramrepus
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1037278 - 11/08/02 06:23 PM (21 years, 7 months ago)

Anno, thanks for doing that tek!

I found the tek to be very useful, and it works very well.

I just have one thing id change. The verm:brf ratio seems to be lacking as far as BRF is concerned, maybe its just me? Your cambo's seem to have taken to it greatly, but I found when I use more BRF it colonizes faster. could this just be other factors?

Besides that, the tek is awesome, and better than MMGG or original PF-Tek in my opinion, just because it cuts out a lot of the bullshit.

Good job

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OfflineAnnoA
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Re: P. cubensis and P. azurescens jars [Re: tekramrepus]
    #1038060 - 11/09/02 12:46 AM (21 years, 7 months ago)

>The verm:brf ratio seems to be lacking as far as BRF is concerned, maybe its just me?

The amount of BRF is settled between the fanaticus?original formula ( 1/8 cup=0.125 cup) and the "maximum yield" formula(1/4 cup=0.25 cup).
I use 0.17 cup.
The reason I don?t use more is that I found that this amount is the best for the method of preparation I use, namely soaking the vermiculite to the field capacity first, then adding BRF.
If you use somewhat more BRF(which you allways can), you?d have to add more water, and the tek wouldn?t be that easy for the newbie, since you can?t really tell by looking at the substrate if it?s wet enough or too wet, if you?re doing it for your first time.

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OfflineJovialLeprechaun
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1038103 - 11/09/02 01:16 AM (21 years, 7 months ago)

Anno, I'm sure you've heard it many times before, but you are a very great person.

You added the millet the PF cake right?


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Edited by JovialLeprechaun (11/09/02 01:17 AM)

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OfflineAnnoA
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Re: P. cubensis and P. azurescens jars [Re: JovialLeprechaun]
    #1038116 - 11/09/02 01:22 AM (21 years, 7 months ago)

>You added the millet the PF cake right?

Yes, I added some millet to it.

Note, that if you add some other grain than BRF, you have to pressure cook the jars for a full hour.

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OfflineJovialLeprechaun
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1038162 - 11/09/02 01:44 AM (21 years, 7 months ago)

Thanks for the extra clarification, I have a PC myself, but I'd hate for my question to cause someone to mess that up.


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OfflineAnnoA
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Re: P. cubensis and P. azurescens jars [Re: JovialLeprechaun]
    #1097938 - 11/30/02 06:07 AM (21 years, 6 months ago)

After 1 month of colonisation the jar is nearly done.


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Anonymous

Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1098001 - 11/30/02 07:40 AM (21 years, 6 months ago)

- Post History Deleted Upon User's Request -

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OfflineAnnoA
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1133967 - 12/12/02 01:25 AM (21 years, 6 months ago)

Since a few days the P. azurescens jar has been completely colonized:




As a contrast, a P. cubensis Ecuador jar 10 days after inoculation:



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OfflineDickie
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Registered: 06/03/02
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Re: P. cubensis and P. azurescens jars [Re: Anno]
    #1135248 - 12/12/02 10:21 AM (21 years, 6 months ago)

Very nice Anno!
So the Azures took almost a month and a half to colonize?
Great pictures what kind of camera do you have?


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