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OfflineVoodoo486
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Registered: 12/13/08
Posts: 159
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Easy Cloning
    #10320256 - 05/11/09 12:28 AM (14 years, 10 months ago)

Can you clone a fruit by cutting a piece of tissue from the fruit and putting it into a sterilized grain jar?

I have seen some "easy clone" teks that talk about putting the piece of tissue into an LC but what about putting it directly into a grain jar?


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Offlinebeans
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Registered: 03/31/09
Posts: 121
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Re: Easy Cloning [Re: Voodoo486]
    #10320259 - 05/11/09 12:29 AM (14 years, 10 months ago)

yes you could do that. Just make sure you do it in a glove box or sterile environment

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OfflineTechn9cian
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Re: Easy Cloning [Re: beans]
    #10320288 - 05/11/09 12:38 AM (14 years, 10 months ago)

ive been told you dont get a true clone cause that piece could contain more than one strain of mycelium, but i've done it so start a new culture


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OfflineVoodoo486
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Re: Easy Cloning [Re: Techn9cian]
    #10320304 - 05/11/09 12:43 AM (14 years, 10 months ago)

The not 100% true clone thing could happen, yes, but that's okay. I'm trying to isolate without agar and I have also been having a terrible time with Honey/Karo LC's. I want to see if I can solve two problems at once by cloneing with a fruit's tissue, transferring it to grain jar, and then following the grain LC tek.


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Invisiblevirus1824
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Registered: 09/25/05
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Re: Easy Cloning [Re: Voodoo486]
    #10320689 - 05/11/09 02:48 AM (14 years, 10 months ago)

I'd start out with petri dishes if i were you. the chances of failure are greatly increased if you just dump it in a grain jar. Colonization will take alot longer than just growing on petri's and putting 3 wedges in a grain jar.


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InvisibleBlimeyGrimey
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Registered: 08/24/05
Posts: 3,796
Loc: Puget Sound
Re: Easy Cloning [Re: virus1824]
    #10320781 - 05/11/09 03:41 AM (14 years, 10 months ago)

Quote:

virus1824 said:
I'd start out with petri dishes if i were you. the chances of failure are greatly increased if you just dump it in a grain jar. Colonization will take alot longer than just growing on petri's and putting 3 wedges in a grain jar.




:thumbup:


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