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Offlinedoornoblightbulb
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Is this mycelium growth or just a contaminant?
    #10198744 - 04/19/09 11:22 PM (14 years, 10 months ago)

Note: I'm just kinda fucking around here. Experimenting.

I filled 2 water bottles with boiled honey water. Then I threw in a little shroom stem into each bottle. This was like 5 days ago. I have been storing the bottles in my closet, which is dark, but not very warm, which would slow the growth.

Now some stuff is growing in there. I'm not sure what it is. I have no idea what mycelium growth looks like when in water. Does it float or sink? Cling together or spread apart? I have no idea. So I have 2 questions for you guys.

First off, is this mycellium growing in here, or is it just bacteria or some other contam?

AND, what are the characteristics of mycellium growth in honey water (so I don't have to make this thread again.)

Thanks.

Pics:
Jar #1 -



Jar #2 -



These pics aren't the best quality. There is a lot more growth than can be seen in these pics.

Edited by doornoblightbulb (04/19/09 11:24 PM)

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OfflineJohnny Quest
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Re: Is this mycelium growth or just a contaminant? [Re: doornoblightbulb]
    #10198845 - 04/19/09 11:47 PM (14 years, 10 months ago)

Behold the problem with LCs...You'll never know if that is a contam 'till you inoculate. Also there may be some invisible mold spores (or other sort of nastiness) that may not reveille itself until it starts growing on your PF cake.

That being said...I wish you the best of luck.  I hope its some myc!


--------------------
Studying mycology since: August, 2008

A typical monotub grow of a multi-spore rye spawn with some Golden Teachers: inoculated on day one and saw first signs of growth on day 5. On day 17 jars were 15% - 30% colonized so shook em up. On day 25 I mixed the spawn and sub in the monotub and on day 50 harvested the first flush. Just under 4 ounces dry in 1 month 19 days!

One Love!

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OfflineNightfly
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Re: Is this mycelium growth or just a contaminant? [Re: doornoblightbulb]
    #10198943 - 04/20/09 12:07 AM (14 years, 10 months ago)

>Does it float or sink?

sinks.

>Cling together or spread apart?

clings together.

I can't tell what it is from the pics, but next time I would use glass so u can at least sterilize the container. Also you might have a hard time getting the myc out of that bottle unless u have a huge syringe needle, use a small glass jar next time.

Edited by Nightfly (04/20/09 12:08 AM)

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Offlinedoornoblightbulb
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Re: Is this mycelium growth or just a contaminant? [Re: Nightfly]
    #10198994 - 04/20/09 12:18 AM (14 years, 10 months ago)

I'm just going to jam a syringe through the bottom of the bottle and suck up anything that could be mycellium. Hopefully it works. If it does that would be cool.

From .1 grams of dried, old, shrooms ----> A fresh flush. And all without a pressure cooker.

(I did just buy a pressure cooker today though anyways)

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Offlinedoornoblightbulb
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Re: Is this mycelium growth or just a contaminant? [Re: doornoblightbulb]
    #10200630 - 04/20/09 09:48 AM (14 years, 10 months ago)

Do you think I should let it grow more before I use it?

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Offlinedoornoblightbulb
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Re: Is this mycelium growth or just a contaminant? [Re: doornoblightbulb]
    #10203109 - 04/20/09 06:14 PM (14 years, 10 months ago)

K, some of this growth is floating, and some of it sinks. Does this mean that only the growth on the bottom my potentially be mycellium? And that growth on the top is just a contam?

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Offlineveda_sticks
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Re: Is this mycelium growth or just a contaminant? [Re: doornoblightbulb]
    #10203174 - 04/20/09 06:29 PM (14 years, 10 months ago)

Your chances of that being a cleen LC are slim. Since you didnt properly sterilise your LC and you used a peice of old dried shroom tissue, which will likely be covered in contaminants.

dried tissue will infact start growing again hen hydrated, but it needs to be done on agar so that you can isolate away from contamination.


www.mushroomvideos.com

start there

Im not a fan of LC, id rather do grain to grain transfers


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlineoh_you_know
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Re: Is this mycelium growth or just a contaminant? [Re: veda_sticks]
    #10203205 - 04/20/09 06:35 PM (14 years, 10 months ago)

veda_sticks is all about tellin people you are gonna fail, if youre just fucking around,who cares? what are you gonna lose? nothing. put it in a jar, if it works then it works if not then toss it. just experiment and see what happens


--------------------
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Offlinedoornoblightbulb
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Re: Is this mycelium growth or just a contaminant? [Re: oh_you_know]
    #10203313 - 04/20/09 06:58 PM (14 years, 10 months ago)

Quote:

oh_you_know said:
veda_sticks is all about tellin people you are gonna fail, if youre just fucking around,who cares? what are you gonna lose? nothing. put it in a jar, if it works then it works if not then toss it. just experiment and see what happens




Ya exactly. First thing I said was I'm just fucking around.

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Offlineveda_sticks
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Re: Is this mycelium growth or just a contaminant? [Re: oh_you_know]
    #10203347 - 04/20/09 07:06 PM (14 years, 10 months ago)

no, im all about telling people tried and tested methods that work 99% of the time. Theres no point in getting someones hopes up, for them to end up with failure and get discouraged.

This hobby isnt free and most of us dont make money out of it. Why waste time and good resources that cost money.

Im all for experimenting.  You must not have read my thread where i inoculated a heavily bacteria contaminated jar with oak ridge spores - which some people say can eat contamination. It then got contaminated with green mould. Then a week later has mycelium growing. Its apearing to be eating the contamination. I doubt that jar will succeed. And RR replied saying it looked like bacteria.

My adive would be to let it grow out and in the meanwhile start up some pf jars using proven techniques. Once the LC has colinised enough to get mycelial water, test it in 1 jar.

But i wouldnt expect much.


My post just pointed out the chances of success, and linked to a proven method. So that he didnt put all his eggs in 1 basket and loose out.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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InvisibleMEEZIE
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Re: Is this mycelium growth or just a contaminant? [Re: doornoblightbulb]
    #10203369 - 04/20/09 07:10 PM (14 years, 10 months ago)

Drink it. If it taste like sun flower seeds, its mycelium. If it taste like appricot, or pennies its a contam

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InvisibleMEEZIE
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Re: Is this mycelium growth or just a contaminant? [Re: MEEZIE]
    #10203376 - 04/20/09 07:11 PM (14 years, 10 months ago)

Thats Mike's LC Taste Test TEK

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Offlineveda_sticks
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Re: Is this mycelium growth or just a contaminant? [Re: MEEZIE]
    #10203397 - 04/20/09 07:15 PM (14 years, 10 months ago)

LOL


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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InvisibleMEEZIE
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Registered: 02/28/09
Posts: 711
Loc: USA Flag
Re: Is this mycelium growth or just a contaminant? [Re: veda_sticks]
    #10203435 - 04/20/09 07:21 PM (14 years, 10 months ago)

Those were the first three "tastes" that came to my head for some reason. Dont ask me why.

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OfflineJohnny Quest
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Re: Is this mycelium growth or just a contaminant? [Re: MEEZIE]
    #10304277 - 05/07/09 07:12 PM (14 years, 10 months ago)

LOL on Mikes taste test tek!

And Veda: I don't care what people say...you're a badass and you've never been less than helpful when helpin' me out on my grows! I really appreciate your input!


--------------------
Studying mycology since: August, 2008

A typical monotub grow of a multi-spore rye spawn with some Golden Teachers: inoculated on day one and saw first signs of growth on day 5. On day 17 jars were 15% - 30% colonized so shook em up. On day 25 I mixed the spawn and sub in the monotub and on day 50 harvested the first flush. Just under 4 ounces dry in 1 month 19 days!

One Love!

Extras: Filter Print Post Top
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