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Shop: Mushroom-Hut Substrate Mix   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order

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Offlinefunguy305
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13 weeks into inoculation of B+ brf and I am starting to see...
    #10277853 - 05/03/09 11:49 AM (14 years, 10 months ago)

Some dark spots in some of the jars.  Hard to tell if it is dark blue, dark green or black.  Can contamination take this long to start?  The jars are about 80% colonized.


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new to the shroom so thanks for all the help.

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Offlineiskinbash
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: funguy305]
    #10277937 - 05/03/09 12:09 PM (14 years, 10 months ago)

If you're thirteen weeks in and your jars aren't fully colonized already, you hit problems long ago.


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*iskinbash is merely a fictional character placed in a real life environment.

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Offlinefunguy305
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: iskinbash]
    #10278205 - 05/03/09 01:12 PM (14 years, 10 months ago)

Well I got 2 cakes dunked and rolled in the fruiting chamber 2 weeks ago.  Another just fully colonized 2 days ago and is still in the jar.  I just pulled the tape off the inoc holes on the last jars.  For the ones that are not fully colonized is there still a chance?  What about the ones with the dark spots?


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new to the shroom so thanks for all the help.

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Offlineiskinbash
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: funguy305]
    #10278294 - 05/03/09 01:34 PM (14 years, 10 months ago)

Tape over the holes may have been the reason for slow colonization. Your jars need to breathe. What kind of tape did you use? Any dark growth in your jars is not good. To be honest, it should have taken up to 4 weeks to fully colonize a 1/2 pint brf cake.


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*iskinbash is merely a fictional character placed in a real life environment.

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Offlinemykalt45
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: iskinbash]
    #10278305 - 05/03/09 01:36 PM (14 years, 10 months ago)

Bruised mycelium can turn blue.

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Offlineiskinbash
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: mykalt45]
    #10278504 - 05/03/09 02:10 PM (14 years, 10 months ago)

It's hard to bruise myc while the cake is still in the jar


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*iskinbash is merely a fictional character placed in a real life environment.

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OfflineABC
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: iskinbash]
    #10278763 - 05/03/09 03:06 PM (14 years, 10 months ago)

Throw that in a pot of boiling water for a half hour to kill all the gross stuff before you open it to dump it out

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Offlinefunguy305
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: ABC]
    #10279178 - 05/03/09 04:43 PM (14 years, 10 months ago)

I used the medical bandage tape, I thought that would help it breath and keep it safe from contamination.  The dark spots just showed up in the jars before the tap


--------------------
new to the shroom so thanks for all the help.

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Offlinefunguy305
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: funguy305]
    #10279205 - 05/03/09 04:47 PM (14 years, 10 months ago)

As for the bruising, I did tap the cakes loose from the sides of the jar when I gave them a flip.  But I do not think these spots are from that, but I am new and still learning.


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new to the shroom so thanks for all the help.

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Offlineiskinbash
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: funguy305]
    #10279664 - 05/03/09 06:17 PM (14 years, 10 months ago)

Was the tape you used micropore surgical tape? There are lots of different medical tapes.


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*iskinbash is merely a fictional character placed in a real life environment.

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Offlinefunguy305
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: iskinbash]
    #10284911 - 05/04/09 04:43 PM (14 years, 10 months ago)

Just looked at the tape and it is water proof bandage tape.  Guess not to much breathing through that huh?  The last jars have really taking off with mycylium growth since the tape came off.  If they stay at this rate of growth they should be fully colonized at the end of the week.  The spots do seem to be turning bluish now as well.  For the 2 in the fruiting chamber (near 100% humidity, 76-79 degrees and 12/12 lite on/off)  it has been 2+ weeks now.  Fruit anytime soon????


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new to the shroom so thanks for all the help.

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Offlineiskinbash
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: funguy305]
    #10289417 - 05/05/09 10:20 AM (14 years, 10 months ago)

Yup. that tape was the culprit for slow colonization.

After 2+ weeks, you should be seeing some pins by now...but Ive heard of cakes taking longer than that to pin. Have you seen anymore mycelial growth since you placed them in the fruiting chamber? If so, just be patient. Mother nature likes to take her sweet ass time sometimes.


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*iskinbash is merely a fictional character placed in a real life environment.

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Offlineelectrics
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: iskinbash]
    #10289439 - 05/05/09 10:26 AM (14 years, 10 months ago)

You may have thrown the overall time-line of by you're jars expending so much energy just to completely colonize I would suggest starting some new jars and leaving the holes open for GE remember you still have you're dry verm layer for protection..I am no expert but would guess the mycelium used up alot of the nutrients trying to colonize so there may not be many left for the fruits??E:2cents:


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"Listen now I'm talking I've been here for weeks, waiting in this growing crowd staring at my feet, The world around me is Turning I'm just standing still, The time has come for changes do something or I will" Phish

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Offlineiskinbash
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: electrics]
    #10290451 - 05/05/09 01:32 PM (14 years, 10 months ago)

yeah, trying to colonize without being able to breathe may have made the myc not so healthy (not certain though). and that may be why your cakes are taking forever to fruit. Hope for the best but expect the worst. I would think myc to be resilient enough to make a comeback, but it may take some time. In the meantime, start some new jars. And for the future, the dry verm layer serves very well as your contam barrier, but if youre really worried about it, be sure to get some micropore tape. Dont get it unless it says "Micropore" on it. Prob small letters on the back. its a paper tape.


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*iskinbash is merely a fictional character placed in a real life environment.

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Offlinefunguy305
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: iskinbash]
    #10291040 - 05/05/09 03:23 PM (14 years, 10 months ago)

The cakes in the fruitng chamber are still showing mycylial growth.  But has not covered the perlite 100% since the roll.  Should I put them back in the dark and raise the temp till 100% covered?
Thanks iskinbash and electrics!


--------------------
new to the shroom so thanks for all the help.

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Offlineiskinbash
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Registered: 09/25/08
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: funguy305]
    #10291217 - 05/05/09 03:55 PM (14 years, 10 months ago)

Don't do that. You rolled them in perlite?? You should have rolled them in vermiculite. Got any pics I can see?


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*iskinbash is merely a fictional character placed in a real life environment.

Edited by iskinbash (05/05/09 04:05 PM)

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Offlinefunguy305
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: iskinbash]
    #10292959 - 05/05/09 07:58 PM (14 years, 10 months ago)

Sorry I got the two confused.  It was the vermiculate that I used for the roll. (Damn Newbie)
These are the 2 cakes that have been dunked and rolled and have been in the fruiting chamber for almost 3 weeks now.  I have an older camera so these were the best I could do.  What do you think?


--------------------
new to the shroom so thanks for all the help.

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Offlineiskinbash
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Registered: 09/25/08
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Last seen: 7 years, 5 months
Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: funguy305]
    #10293078 - 05/05/09 08:10 PM (14 years, 10 months ago)

if you see growth still, that is a good thing. Has the myc been expanding since you put them in fruiting, or have they just begun showing myc growth within the last week?


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*iskinbash is merely a fictional character placed in a real life environment.

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Offlinefunguy305
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Registered: 04/07/09
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: iskinbash]
    #10295399 - 05/06/09 05:25 AM (14 years, 10 months ago)

the myc has increased everyday since placed in the fruiting chamber.  Not fast but steady.  The pics do not show it but there is rhizo around the bottoms of the cakes reaching out.


--------------------
new to the shroom so thanks for all the help.

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Offlinefunguy305
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Registered: 04/07/09
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Re: 13 weeks into inoculation of B+ brf and I am starting to see... [Re: funguy305]
    #10295406 - 05/06/09 05:29 AM (14 years, 10 months ago)

again I would like to thank you (iskinbash) for your help and patience with this newbie!!


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new to the shroom so thanks for all the help.

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