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Amazon Shop for: ½ Pint Jars, HEPA Filter, Rye Grain, Wild Bird Seed

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Anonymous

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    #1029288 - 11/06/02 07:55 PM (14 years, 2 months ago)



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Invisiblepsyphon
mneumatic device

Folding@home Statistics
Registered: 11/27/01
Posts: 565
Re: Rye question [Re: ]
    #1029789 - 11/06/02 09:43 PM (14 years, 2 months ago)

As long as you don't smell something rancid from the jars, it should be fine.  I find grain to grain to be pretty easy.  It sounds like you might not need to, but what I do is take some empty syringes and repeatedly fill and empty them with boiling water to sterilize them.  Leave them filled the final time and let cool.  Then squirt out half or more of the water and fill the rest with Hydrogen Peroxide.  Then I squirt this over the grain I'm transfering to and make the transfer.  I wait for growth to start into the new grain before shaking.  This works great for me so if you do run into any problems (I hope you don't) give it a try.

Good luck :smile: 


--------------------
"The real voyage of discovery consists not in seeking new landscapes but in having new eyes."
- Marcel Proust

I wish you all ceaselessly flowing moments of happiness.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Rye question [Re: ]
    #1031335 - 11/07/02 05:17 AM (14 years, 2 months ago)

You should be sure the mother jar is a firm 100% colonized, before using it for G2G transfers. When 100% colonized, the grain is a solid clump, you have to dig out - with a spoon. One quart will get you about 20 more, just using two table spoons full, into each new jar.

Oven tek is BS.  :shocked:


Picture (c) ANNO

6T Grain to Grain (G2G) Transfer method  :wink:

Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.

The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.

Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you don't have one. You can usually manage without it

Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask  (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.

Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about ? of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting its filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.

Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it.

Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3 to recuperate from that shock.  There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.

6T  :tongue:


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


Edited by SixTango (11/07/02 05:26 AM)


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Amazon Shop for: ½ Pint Jars, HEPA Filter, Rye Grain, Wild Bird Seed

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