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Stealthgrower
n00b Pwner



Registered: 04/19/09
Posts: 622
Last seen: 12 years, 11 months
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Re: Micropore tape instead of tyvek on lids [Re: FooMan]
#10290405 - 05/05/09 01:25 PM (14 years, 8 months ago) |
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Quote:
FooMan said:
Quote:
Stealthgrower said: So does that mean you just poke through the tyvek and that's it? no tape or anything? I haven't tried tyvek but I assume it would leave a hole.
You would definitely use micropore over the hole if you were to inoculate through it.
Ok, that's what I thought originally, BUT, there would be a gap between your tyvek needle hole and the tape right? Because the metal lid is in between the two. Therefore, something could go through the OTHER metal hole, travel across the TOP of the tyvek lid UNDER the metal lid, and go through the tyvek needle hole. Right? How would you prevent this?
-------------------- Infea said: You ever seen that movie Constantine. Well they pass into hell several times. In hell there are melting human bodies, in your tub are melting mushroom bodies. Your tub had passed into mushroom hell.
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Captain Caveman
Uh-oh!



Registered: 01/12/09
Posts: 2,253
Last seen: 1 month, 4 days
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Re: Micropore tape instead of tyvek on lids [Re: Stealthgrower]
#10290421 - 05/05/09 01:27 PM (14 years, 8 months ago) |
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Its nothin so significant to worry about. What does it matter since we have already shown that micropore tape is a good barrier on its own?
-------------------- "Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"
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Stealthgrower
n00b Pwner



Registered: 04/19/09
Posts: 622
Last seen: 12 years, 11 months
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Re: Micropore tape instead of tyvek on lids [Re: Captain Caveman]
#10290465 - 05/05/09 01:34 PM (14 years, 8 months ago) |
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Captain you are missin it man, so I made you an illustration.... The needle makes an obvious hole, and leaves a path for contams, despite the placement of the tape on the inoc. site.
-------------------- Infea said: You ever seen that movie Constantine. Well they pass into hell several times. In hell there are melting human bodies, in your tub are melting mushroom bodies. Your tub had passed into mushroom hell.
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atomic1
enthusiast



Registered: 09/18/03
Posts: 1,123
Loc: Appalachia
Last seen: 4 years, 3 months
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Re: Micropore tape instead of tyvek on lids [Re: Stealthgrower]
#10290590 - 05/05/09 01:56 PM (14 years, 8 months ago) |
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I think what captain was trying to say is that you're starting to split hairs. Of course there will be a lil hole for 2 sec. til you can put another piece of tape over it but its inevitable. Thats why he said it was nothing so significant to worry about. However, you can go buy some high temp. silicone and make your own self-sealing injection port. Problem solved.
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nicechrisman
Interdimensional space wizard



Registered: 11/07/03
Posts: 33,241
Last seen: 4 years, 6 months
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Re: Micropore tape instead of tyvek on lids [Re: Stealthgrower]
#10290621 - 05/05/09 02:01 PM (14 years, 8 months ago) |
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I get what you're saying. It's not the injection hole with tape over it, it's the other hole that they can enter though, go between the lid and tyvek, then through the injection hole. Like the drawing.
-------------------- "Cosmic Love is absolutelely ruthless and highly indifferent: it teaches its lessons whether you like/dislike them or not." John C. Lily
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Stealthgrower
n00b Pwner



Registered: 04/19/09
Posts: 622
Last seen: 12 years, 11 months
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Re: Micropore tape instead of tyvek on lids [Re: nicechrisman]
#10290644 - 05/05/09 02:05 PM (14 years, 8 months ago) |
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Quote:
nicechrisman said: I get what you're saying. It's not the injection hole with tape over it, it's the other hole that they can enter though, go between the lid and tyvek, then through the injection hole. Like the drawing.
exactly. you aren't ACTUALLY putting tape on the tyvek, just on the metal ABOVE the tyvek.
-------------------- Infea said: You ever seen that movie Constantine. Well they pass into hell several times. In hell there are melting human bodies, in your tub are melting mushroom bodies. Your tub had passed into mushroom hell.
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atomic1
enthusiast



Registered: 09/18/03
Posts: 1,123
Loc: Appalachia
Last seen: 4 years, 3 months
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Re: Micropore tape instead of tyvek on lids [Re: Stealthgrower]
#10290698 - 05/05/09 02:13 PM (14 years, 8 months ago) |
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There's nothing you can do about it.......period. The only thing you can do is avoid it by finding someone that sells the lids with injection ports in them or make your own. Its not that big of a deal. People have had this happen for years and years. Just be careful and as sterile as possible and you'll be fine.
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nicechrisman
Interdimensional space wizard



Registered: 11/07/03
Posts: 33,241
Last seen: 4 years, 6 months
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Re: Micropore tape instead of tyvek on lids [Re: atomic1]
#10290944 - 05/05/09 03:05 PM (14 years, 8 months ago) |
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If you put the tyvek over the lid, then you can completely seal the opening.
-------------------- "Cosmic Love is absolutelely ruthless and highly indifferent: it teaches its lessons whether you like/dislike them or not." John C. Lily
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atomic1
enthusiast



Registered: 09/18/03
Posts: 1,123
Loc: Appalachia
Last seen: 4 years, 3 months
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Re: Micropore tape instead of tyvek on lids [Re: nicechrisman] 1
#10290985 - 05/05/09 03:13 PM (14 years, 8 months ago) |
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So what exactly are we debating now? This thread us starting to make me friggin crazy.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Micropore tape instead of tyvek on lids [Re: Stealthgrower]
#10291686 - 05/05/09 04:59 PM (14 years, 8 months ago) |
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Quote:
Stealthgrower said:
Quote:
nicechrisman said: I get what you're saying. It's not the injection hole with tape over it, it's the other hole that they can enter though, go between the lid and tyvek, then through the injection hole. Like the drawing.
exactly. you aren't ACTUALLY putting tape on the tyvek, just on the metal ABOVE the tyvek.
No, you are putting the micropore on the hole in the tyvek. The micropore isn't rigid. when you apply the tape it would have a concave shape with the center sticking to and around the hole while the edges would be slightly raised and sticking to the lid. Thanks for the diagram. I had no clue what you were talking about before The chances of what you're talking about happening are slim. I've never had that happen to me before and I've done that method more than a few times.
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Quick WBS Prep
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1stusinspace
I love the sound of purple



Registered: 02/06/09
Posts: 139
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Re: Micropore tape instead of tyvek on lids [Re: Stealthgrower]
#10291739 - 05/05/09 05:07 PM (14 years, 8 months ago) |
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Quote:
Stealthgrower said: Captain you are missin it man, so I made you an illustration.... The needle makes an obvious hole, and leaves a path for contams, despite the placement of the tape on the inoc. site.

Just put the micropore tape over both holes in the lid when your done,thats what i do.
-------------------- A bus came by and I got on
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xail
Trip McGee


Registered: 05/30/08
Posts: 20
Last seen: 13 years, 2 months
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Re: Micropore tape instead of tyvek on lids [Re: Stealthgrower]
#10292165 - 05/05/09 06:10 PM (14 years, 8 months ago) |
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tyvek + silicon + gorilla tape = OMGWTFPWN
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Micropore tape instead of tyvek on lids [Re: xail]
#10292229 - 05/05/09 06:17 PM (14 years, 8 months ago) |
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You guys are way overthinking this stuff. K.I.S.S.
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Quick WBS Prep
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palidon11
Is there anybodyout there?


Registered: 12/21/07
Posts: 33
Loc: Wisconsin!
Last seen: 14 years, 7 months
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Re: Micropore tape instead of tyvek on lids [Re: Captain Caveman]
#10292285 - 05/05/09 06:26 PM (14 years, 8 months ago) |
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i put bandaids on my lids because i didn't have micropore tape or tyvek. hasn't failed yet, although next time i'll do it properly...
mycelium be growin!
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beans
Stranger Danger!

Registered: 03/31/09
Posts: 121
Last seen: 14 years, 7 months
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Re: Micropore tape instead of tyvek on lids [Re: palidon11]
#10292829 - 05/05/09 07:41 PM (14 years, 8 months ago) |
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I have read a lot of FooMans posts and I am willing to bet that he has tons of experience. If this method didn't work, he probably wouldn't do it or recommend it. If it really bothers you about the gap, then cut your tyvek so it hangs a little past the ring and around the jar. Then when you go to inoculate, remove the band and lid (leaving just the filter). Inject through that and tape immediately afterwards right on the tyvek securing the hole. Put your lid back on and your golden. I think you are over analyzing, but that just my own opinion
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