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Offlinemushroomhunter10
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Anybody think that golf tees would imporve spore print sterility?
    #10276688 - 05/03/09 03:22 AM (14 years, 8 months ago)

Would it help to poke a golf tee through the center of the cap's underside and use the tee upside-down to keep the cap off of the printing medium?

Thanks


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Edited by mushroomhunter10 (05/03/09 03:22 AM)


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InvisibleLouiseLouise
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Re: Anybody think that golf tees would imporve spore print sterility? [Re: mushroomhunter10]
    #10276702 - 05/03/09 03:30 AM (14 years, 8 months ago)

How would that help?


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"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC


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Offlinemushroomhunter10
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Re: Anybody think that golf tees would imporve spore print sterility? [Re: LouiseLouise]
    #10276709 - 05/03/09 03:31 AM (14 years, 8 months ago)

Well if wild prints have contaminants on the cap.

I guess for huge caps it would be wobbly though.

Maybe it's overboard, maybe I'm stoned.


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES


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InvisibleLouiseLouise
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Re: Anybody think that golf tees would imporve spore print sterility? [Re: mushroomhunter10]
    #10276735 - 05/03/09 03:45 AM (14 years, 8 months ago)

Lifting it while it is dropping spores would increase the chances of wanted spores escaping and unwanted spores entering. Not to mention that the media would already be clean, right?
It does help to use a thumbtack, toothpick or pin to poke into the top to lift it off of the medium while printing, golf tee seems a little heavy tho :smirk:


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"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC


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Offlinemushroomhunter10
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Re: Anybody think that golf tees would imporve spore print sterility? [Re: LouiseLouise]
    #10276749 - 05/03/09 03:51 AM (14 years, 8 months ago)

Yeah but don't you print in a still air environment?

And it would only be an 1/8" off of the medium.


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES


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InvisibleinskiM
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Re: Anybody think that golf tees would imporve spore print sterility? [Re: mushroomhunter10] * 3
    #10276770 - 05/03/09 04:09 AM (14 years, 8 months ago)

I cut a small square of stainless mesh from a sieve and fold each corner over so it sits just of the deck, about 2mm, this way nothing comes into contact with the foil apart from spores, I sit the severed pileus on the mesh and make an initial print on paper for an hour or so under a glass or plastic container then slide the paper out without lifting the container, I then slide a clean piece of foil under and make the final print, I wear gloves and a mask for the whole procedure!
I have had very good success with this technique and have been able to create clean cultures with no transfers needed, even from wild mushrooms!
inski.


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Invisiblenightflyer
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Re: Anybody think that golf tees would imporve spore print sterility? [Re: inski]
    #10276981 - 05/03/09 06:34 AM (14 years, 8 months ago)

I can confirm it works great.
I have tested inski's technique two weeks ago.  :thumbup:


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Offlinemushroomhunter10
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Re: Anybody think that golf tees would imporve spore print sterility? [Re: nightflyer]
    #10277761 - 05/03/09 11:17 AM (14 years, 8 months ago)

inski:

Do you have a picture?? I can't imagine what you're describing. :shrug:


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES


Extras: Filter Print Post Top
Offlinemushroomhunter10
Jack-Of-All-Trades
Male User Gallery


Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 8 years, 7 months
Re: Anybody think that golf tees would imporve spore print sterility? [Re: mushroomhunter10]
    #10279385 - 05/03/09 05:21 PM (14 years, 8 months ago)

I see, a screen. I was stoned, and nodding SEVERELY! :grin:

It must be some sturdy mesh to be able to have its corners folded and support a cap.


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES


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