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lipa
Registered: 07/24/07
Posts: 2,684
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Calvatia subcretacea
#10263503 - 04/30/09 03:55 PM (14 years, 10 months ago) |
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Calvatia subcretacea
An attempt to fruit Calvatia subcretacea.
Culture from a multi-spore selection
A mixture of rye grass seed, coir,sawdust, worm castings, and sand was inoculated with a wedge and allowed to "aggressively" colonize for 6 days. After a week of dormancy I placed a 1/2 inch layer of dry paper pellets on top to hopefully simulate a partly arid environment and help it on its way into fruit body formation. The mycelium was very efficient in channeling moisture to the dry pellets and within 6 days the pellets were colonized.
Shortly thereafter(3 days) i started to notice large round swelling on top of the substrate and this over the period of 1 week formed what was looking very similar to a immature puffball. I had the humidity in the 95-100% range for this period and noticed the fruitbody was getting rather wet so I decided to lower the humidity to around 80%. A noticeable improvement occurred and a new fruit body started forming right on top of the first probably due to the shift in humidity. It seems to be very sensitive to its environment. Seems to be doing very well now and I will keep you posted on what happens.If any of you have any experience with growing any type of puffball I would really appreciate your comment.
Pot ash seems to stimulate primordia formation on agar.
Aggressively colonizing ash
Edited by lipa (04/30/09 03:59 PM)
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ABC
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Re: Calvatia subcretacea [Re: lipa]
#10263702 - 04/30/09 04:31 PM (14 years, 10 months ago) |
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Woah! I wonder what a puffball monotub would look like
So cool...
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Mephistophelian
Quasi Hob-Nobbery
Registered: 08/14/08
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Re: Calvatia subcretacea [Re: ABC]
#10275351 - 05/02/09 09:17 PM (14 years, 10 months ago) |
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That's facinating.
What did you use as your fruiting container?
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lipa
Registered: 07/24/07
Posts: 2,684
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Just a deli cup..
Lipa
Edited by lipa (05/03/09 12:12 PM)
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falcon
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Re: Calvatia subcretacea [Re: lipa]
#10278048 - 05/03/09 12:35 PM (14 years, 10 months ago) |
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Holy Fruiting Puffballs Lipa!
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MycoAu
5thKingdomCome
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Re: Calvatia subcretacea [Re: falcon]
#10279656 - 05/03/09 06:16 PM (14 years, 10 months ago) |
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I didn't know you could cultivate and fruit puffballs. What paper/book/source caused your interest in this species and these parameters?
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Mycelio
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Re: Calvatia subcretacea [Re: lipa]
#10282073 - 05/04/09 03:01 AM (14 years, 10 months ago) |
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Good job, thanks for sharing!
Carsten
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: MycoAu]
#10283026 - 05/04/09 10:25 AM (14 years, 10 months ago) |
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Quote:
What paper/book/source caused your interest in this species and these parameters?
I have a good friend who studies them. Being in the field hunting them has sparked my interest and caused an overwhelming urge to grow them. Mainly to understand them and to understand why they grow as they do. There is not much study on them and it would be of great benefit to my friend and others to grow as many as I possibly can to help in the understanding of their growth cycles and to produce better identification for those specimens that are unsatisfactory and rare but have spores available. They are very fun and from the work I have done so far freakishly aggressive in there vegetative cycle which produces result very quickly. They devour everything I put in their path without hesitation....especially paper products.
The parameters/media? Just using my imagination. I just think like a puffball. What is the environment I am in? How do the conditions where I grow fluctuate throughout the day? How long can I take the low humidity? How is the earth around me and what is it composed of? How in nature do i get my supply of h20 during the dry spells and still manage to stay alive above ground? Why do I prefer some sort of tree and what kind of environment does the tree like and what is unique about it, etc. etc.
Don't bother trying to read up on anything because there isn't much out there. There are soooo many puffballs that have not been found yet or maybe found and overlooked. I love the feeling of working with things that are new and getting the chance to fiqure them out.
Lipa
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caricapapaya
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Re: Calvatia subcretacea [Re: lipa]
#10283496 - 05/04/09 12:42 PM (14 years, 10 months ago) |
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Very nice Lipa. Pushing the limits...
Keep us updated with progress reports.
carica
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Pinback
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Re: Calvatia subcretacea [Re: lipa]
#10290727 - 05/05/09 02:18 PM (14 years, 10 months ago) |
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Coolest post I have seen here for some time! I know ragadinks tried to grow the giant puffball (Calvatia gigantea), but unfortunately I don't think he posts here anymore...?
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Hotnuts
old hand
Registered: 02/26/05
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Re: Calvatia subcretacea [Re: Pinback]
#10307138 - 05/08/09 08:27 AM (14 years, 10 months ago) |
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Are you sure that's a mushroom? It appears to be puffed mycelium. Any updates?
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: Hotnuts]
#10307315 - 05/08/09 09:31 AM (14 years, 10 months ago) |
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Funny you should say that. I am starting to think that Hotnuts. God that name disturbs me! It has taken a kind of conical form as of today. I will try to get some pictures up this afternoon. The thing is almost 3 inches tall now.
Lipa
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Hotnuts
old hand
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Re: Calvatia subcretacea [Re: lipa]
#10307952 - 05/08/09 12:49 PM (14 years, 10 months ago) |
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LOl! I tried to register under my topia name and every other name I could think of and they were all taken. Hotnuts just popped into my mind. Goes to show you how my mind works.
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: Hotnuts]
#10308082 - 05/08/09 01:18 PM (14 years, 10 months ago) |
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Makes me feel so naughty when I say it. Even more so when LOL
Lipa
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obi
Du Bois
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Re: Calvatia subcretacea [Re: lipa]
#10311648 - 05/09/09 05:22 AM (14 years, 10 months ago) |
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Awesome, I've been interested in cultivating them since I came across Calvatia gigantea, they get so friggin'huge, pretty amazing.
Any more pics?
--------------------
To live is to fly
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: Hotnuts]
#10316840 - 05/10/09 11:55 AM (14 years, 10 months ago) |
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Yes, this looks strangely like a puff of mycelium. I am going to let it grow out a little more and then start taking tissue samples for observation. Maybe a form of the fruitbody that is lacking in something? Having a hard time convincing myself it's a puffball.
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Jef
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Re: Calvatia subcretacea [Re: lipa]
#10316931 - 05/10/09 12:14 PM (14 years, 10 months ago) |
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Very cool.
Does look like a gilled mushroom though.
Puffballs...Yummm.
-------------------- I am my own lab rat. Tell me and I will forget. Show me and I will remember. Involve me and I will learn.
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Hotnuts
old hand
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Re: Calvatia subcretacea [Re: Jef]
#10322305 - 05/11/09 12:39 PM (14 years, 10 months ago) |
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Wow, far out! I suppose you'll see what emerges in a few more days. I can't wait to see what happens!
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: Hotnuts]
#10324552 - 05/11/09 09:53 PM (14 years, 10 months ago) |
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Two days from last pic
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MycoAu
5thKingdomCome
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Re: Calvatia subcretacea [Re: lipa]
#10324598 - 05/11/09 10:06 PM (14 years, 10 months ago) |
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not too long from now, you should be able to observe spores inside and a sterile base (all microscopically determined).
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: MycoAu]
#10324625 - 05/11/09 10:13 PM (14 years, 10 months ago) |
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It doesn't even feel like it has an exoperidium. Very fluffy. If it does make spores that WILL be interesting. Seems fragile.
Lipa
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Roger Fudd
Registered: 01/27/09
Posts: 424
Loc: Earth, Inner Solar System
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Re: Calvatia subcretacea [Re: lipa]
#10324755 - 05/11/09 10:41 PM (14 years, 10 months ago) |
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Quote:
lipa said: It doesn't even feel like it has an exoperidium. Very fluffy. If it does make spores that WILL be interesting. Seems fragile.
Lipa
Be like Paul Stamets- throw the puffball! Wait until its mature and full of spores, go to the top of a building, and fire away. Substitute for any situation where a water balloon could be used.
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: Roger Fudd]
#10327231 - 05/12/09 01:22 PM (14 years, 10 months ago) |
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Quote:
Roger Fudd said:
Quote:
lipa said: It doesn't even feel like it has an exoperidium. Very fluffy. If it does make spores that WILL be interesting. Seems fragile.
Lipa
Be like Paul Stamets- throw the puffball! Wait until its mature and full of spores, go to the top of a building, and fire away. Substitute for any situation where a water balloon could be used.
Ummm..... No, I am not going to throw it.
Lipa
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: lipa]
#10360983 - 05/18/09 08:16 PM (14 years, 10 months ago) |
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An update...
I have no freakin clue! I gave it a casing and it is getting soo freakin huge. I keep telling myself "put it outside......put it outside"......but I just keep letting it sit there.
Lipa
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Wingman4l7
Thinks Before He Posts
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Re: Calvatia subcretacea [Re: lipa]
#10361166 - 05/18/09 08:51 PM (14 years, 10 months ago) |
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I've compared the most recent photos above with your earlier ones, and it looks like it is growing a mycelial fuzz on the fruitbody. Maybe it is getting too old? I am reminded of the pinning observed on aging pleurotus fruitbodies, seen here and elsewhere... Lipa, can you confirm what I'm seeing?
Edited by Wingman4l7 (05/18/09 08:57 PM)
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Calvatia subcretacea [Re: Wingman4l7]
#10361207 - 05/18/09 09:00 PM (14 years, 10 months ago) |
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No I don't see it. I've seen that many of times in the garbage after throwing a contaminated block in the trash and visiting it later in the week. This is something else.
I am almost ready to just start cutting it up and dissecting it.
Lipa
Edited by lipa (05/18/09 09:01 PM)
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caricapapaya
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Re: Calvatia subcretacea [Re: lipa]
#10363423 - 05/19/09 08:57 AM (14 years, 10 months ago) |
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That is pretty amazing! if you dissect it be sure to let us know what you find inside
carica
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Mephistophelian
Quasi Hob-Nobbery
Registered: 08/14/08
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Any word on dissection of this creature?
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lipa
Registered: 07/24/07
Posts: 2,684
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I did dissect it and it is definitely mushroom tissue. Was it a puffball fruit body? I haven't convinced myself yet. Upon examining the hyphae in the gleba (probably shouldn't call it that at this point) they were multinucleate and that happens to be the case when I look at other fruitbodies from other species. I took pics but unfortunately I unloaded all of them on my work hard drive which I am trying to recover information off of right now. It seems to be working so hopefully I will be able to post the pics later. it was not producing any spores yet. No coloration. I will be attempting it again on a slightly larger scale and in trays outdoors.
Lipa
Edited by lipa (05/26/09 12:35 PM)
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leschampignons
Biochemistry + Mycology
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Re: Calvatia subcretacea [Re: lipa]
#18822340 - 09/10/13 07:55 AM (10 years, 6 months ago) |
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Nice work. Found this thread during the recent discussion about growing puffballs. Did you ever isolate a fruiting strain from this MS? Do you still have a culture of this species at all?
As I mentioned in a different thread, I collected some gem-studded puffballs and will be trying something similar with them soon. The cultures seem healthy so far.
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vonzvonz
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Re: Calvatia subcretacea [Re: MycoAu]
#26461626 - 01/31/20 06:49 AM (4 years, 1 month ago) |
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Hi Lipa! Do you have any update about the cultivation and fruiting of Calvatia?
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wildernessjunkie
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Re: Calvatia subcretacea [Re: vonzvonz]
#26463043 - 02/01/20 04:34 AM (4 years, 1 month ago) |
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Bro...this thread is 10 years old. Luckily Lipa is still around. But you gotta check those dates.
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vonzvonz
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Hi! Thanks to reply! I knew it was a very old post but I wanted to give it a try. I am starting right now from the scratches. I inoculated some PDA and Complete Media plates with supposed to be Calvatia inoculum on seeds but after 1 week no spores seem to germinate. If Lipa is still around I will be more than happy to discuss with him (her?). I want to try to repeat his experiment as soon I will have some colony growing
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