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 RedDevil420
Antichrist
Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 8 months
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 Agar substitute 
#10199811 - 04/20/09 05:41 AM (14 years, 11 months ago) |
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i have prints that may not be exactly clean. they are fairly good though, and i want to culture them without comtaminates.
the problem is i do not have access to agar at the moment.
If i made a pulp with cardboard at the right water content, and a small bit of flour to help germinate the spores could i successfully transfer away from contams?
If not, how could i germinate my spores and transfer away from contams without agar? i do not need to isolate a strain.
Thanks
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I'm Just Making This All Up.
Grains In Glass Jars With Professional Filters FTW.
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Sterile Technique FTW.
Fan dry, Desiccant storage FTW.
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Duggstar 
Registered: 01/20/09
Posts: 6,273
Loc: Ireland
Last seen: 1 year, 4 months
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You will probably have limited success trying to germinate on cardboard.
Why can you not just order some agar?
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meehi
Being
Registered: 01/11/05
Posts: 311
Last seen: 3 years, 11 months
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Re: Agar substitute [Re: Duggstar]
#10199869 - 04/20/09 06:07 AM (14 years, 11 months ago) |
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Here you go bud, I believe this will solve your problem nicely 
http://www.shroomery.org/8514/Agar-substitute
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Cyber
Ash
Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 11 months, 3 days
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I have tried a lot of agar substitutes but have not had a single one work for me. You are always welcome to try your self but from my experience nothing takes the place of real agar.
On that note, Every Chinese supermarket I have been in carries agar-agar for around 1$ an oz. It is food grade but works fine for mushrooms. If you have a local Chinese Market start there and don't be afraid to ask the guy that runs the place, if he does not carry it he can tell you where to find it.
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Duggstar
Registered: 01/20/09
Posts: 6,273
Loc: Ireland
Last seen: 1 year, 4 months
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Re: Agar substitute [Re: meehi]
#10199887 - 04/20/09 06:16 AM (14 years, 11 months ago) |
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RedDevil420
Antichrist
Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 8 months
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Re: Agar substitute [Re: Duggstar]
#10201189 - 04/20/09 11:43 AM (14 years, 11 months ago) |
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yeah, ill get agar soon. i was just thinking i can start right away if i could mix carboard pulp and a bit of flour (of some sort)
i have seen seen beer soaked cardboard. i could do that. http://www.shroomery.org/forums/showflat.php/Number/742923#742923
i was thinking if i mixed a little bit of flour in it would have enough nutes to germinate and grow out so i can transfer. as long as i didn't use a solid piece of carboard it should be easy to cut a little piece out.
do you think either will work? i already know agar is better.
EDIT: ill try a couple variations tomorrow too, but i want to hear some suggestions.
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I'm Just Making This All Up.
Grains In Glass Jars With Professional Filters FTW.
Coir Monotubs FTW.
Sterile Technique FTW.
Fan dry, Desiccant storage FTW.
Ralphster's Spores FTW.
Mushroom Cloud - Dirtball
Edited by RedDevil420 (04/20/09 11:45 AM)
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Duggstar
Registered: 01/20/09
Posts: 6,273
Loc: Ireland
Last seen: 1 year, 4 months
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What I heard was that this tek is good for propagating mycelium but not germinating spores. But hey, if you don't have any better ideas, it might be an interesting experiment. If you get it to work, let us know. 
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RedDevil420
Antichrist
Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 8 months
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Re: Agar substitute [Re: Duggstar]
#10206090 - 04/21/09 09:37 AM (14 years, 11 months ago) |
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well i made 1 with flour, 1 with sugar and 5 with beer. one collaped haha. i did them in gladware 1/4 pints, microwave sterilization.
we'll see how these work out, i also used paper towel instead of cardboard cuz it was faster. ill throw some spores in later today.
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I'm Just Making This All Up.
Grains In Glass Jars With Professional Filters FTW.
Coir Monotubs FTW.
Sterile Technique FTW.
Fan dry, Desiccant storage FTW.
Ralphster's Spores FTW.
Mushroom Cloud - Dirtball
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RedDevil420
Antichrist
Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 8 months
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alright, i knifed some spores into them. it was a really dark print. ill post if and when i see something.
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I'm Just Making This All Up.
Grains In Glass Jars With Professional Filters FTW.
Coir Monotubs FTW.
Sterile Technique FTW.
Fan dry, Desiccant storage FTW.
Ralphster's Spores FTW.
Mushroom Cloud - Dirtball
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RedDevil420
Antichrist
Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 8 months
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yea no. it didn't do anything but it didn't contam either. I had to get rid of them, so they might have...
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I'm Just Making This All Up.
Grains In Glass Jars With Professional Filters FTW.
Coir Monotubs FTW.
Sterile Technique FTW.
Fan dry, Desiccant storage FTW.
Ralphster's Spores FTW.
Mushroom Cloud - Dirtball
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 German Kahuna
Facepalmer of Stoopid
Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Just work with agar. Don't substitute. Once I started working with agar I never used anything else. It's surprisingly easy, too, once you get used to it.
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"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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RedDevil420
Antichrist
Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 8 months
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yep, thats what i plan to do 
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I'm Just Making This All Up.
Grains In Glass Jars With Professional Filters FTW.
Coir Monotubs FTW.
Sterile Technique FTW.
Fan dry, Desiccant storage FTW.
Ralphster's Spores FTW.
Mushroom Cloud - Dirtball
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