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Invisibledrainhaven
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Petri Wedge or Multispore
    #10206059 - 04/21/09 11:31 AM (11 years, 7 months ago)

K so this isnt really too advanced, perhaps medium.
Anyway we are talking quart jars WBS.

I have to create some more jars, which would be faster. Typical multi-spore inoculation OR dropping a wedge or two in it? I can do grain to lc but will have to wait a week maybe longer.

Thanks

edit: or wedge to liquid culture. Whatever is best.


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Edited by drainhaven (04/21/09 04:32 PM)


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Offlinedjsethall
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Re: Petri Wedge or Multispore [Re: drainhaven]
    #10206287 - 04/21/09 12:33 PM (11 years, 7 months ago)

Wedge to LC. Do a few in case one or more contams. I always do at least three.


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InvisibleBlimeyGrimey
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Re: Petri Wedge or Multispore [Re: djsethall]
    #10207512 - 04/21/09 04:22 PM (11 years, 7 months ago)

Wedge to grain is what I do.

I prefer not to use a syringe when possible. LC require syringes.


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Offlinepotatonet
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Re: Petri Wedge or Multispore [Re: BlimeyGrimey]
    #10211231 - 04/22/09 01:49 AM (11 years, 7 months ago)

steam punkin' it up blimey?

btw I did wedges to WBS, make sure they stay in contact with the wbs because your agar will dry up.


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Invisibledrainhaven
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Re: Petri Wedge or Multispore [Re: potatonet]
    #10211239 - 04/22/09 01:51 AM (11 years, 7 months ago)

What do you mean? Shake it up?


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"We are not human beings having a spiritual experience; we are spiritual beings having a human experience." -Anonymous

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Re: Petri Wedge or Multispore [Re: drainhaven]
    #10211249 - 04/22/09 01:53 AM (11 years, 7 months ago)

just enough shaking to cover it with WBS


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Invisibledrainhaven
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Re: Petri Wedge or Multispore [Re: solumvita]
    #10211332 - 04/22/09 02:08 AM (11 years, 7 months ago)

Yea with grain to grain. I thought he was talking about wedges thats why I was confused.


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"We are not human beings having a spiritual experience; we are spiritual beings having a human experience." -Anonymous

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Offlinepotatonet
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Re: Petri Wedge or Multispore [Re: drainhaven]
    #10211379 - 04/22/09 02:17 AM (11 years, 7 months ago)

the wedges need to be covered, they will dry

get the wedge in there a little deeper than surface


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In the Quechua language of Peru there are over 1000 words for "potato"


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Invisibledrainhaven
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Re: Petri Wedge or Multispore [Re: potatonet]
    #10212676 - 04/22/09 11:25 AM (11 years, 7 months ago)

If Im not concerned about isolation would 3 wedges be more ideal than 1, as far as speed goes?


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Offlinepotatonet
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Re: Petri Wedge or Multispore [Re: drainhaven]
    #10213167 - 04/22/09 01:38 PM (11 years, 7 months ago)

yes,  just make sure when transferring that its sterile as you are opening the jar


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In the Quechua language of Peru there are over 1000 words for "potato"


Edited by potatonet (04/22/09 02:17 PM)


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OfflineRoker
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Re: Petri Wedge or Multispore [Re: potatonet]
    #10234958 - 04/26/09 06:36 AM (11 years, 7 months ago)

wedges, and give it a good shake as well, then leave it alone for a few days (no peeking or shaking). You will get lots of inoculation points this way.


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Offlinepotatonet
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Re: Petri Wedge or Multispore [Re: Roker]
    #10236509 - 04/26/09 03:19 PM (11 years, 7 months ago)

Quote:

Roker said:
wedges, and give it a good shake as well, then leave it alone for a few days (no peeking or shaking). You will get lots of inoculation points this way.





been answered buddy


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In the Quechua language of Peru there are over 1000 words for "potato"


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Offlinecubensis4201
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Re: Petri Wedge or Multispore [Re: drainhaven]
    #10241642 - 04/27/09 11:22 AM (11 years, 7 months ago)

Quote:

drainhaven said:

edit: or wedge to liquid culture. Whatever is best.




If you ever decide to try using an agar wedge to inoculate LC, I like to lightly scrape the mycelium off from the actual agar with a scalpel, or even sometimes a loop. Seem to get much better results rather then just dropping the whole slice(s) in there. Or if you prefer using a syringe, fill it up with sterile water, squirt it on the plate, scrape it around a bit to get the mycelium off the agar, then suck it back up and inject into your LC. Remember that you will get less water back then you put in of course, so add a lil extra in your syringe then you intend to inject in the LC.  Goodluck :smirk:


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Invisiblefastfred
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Re: Petri Wedge or Multispore [Re: cubensis4201]
    #10243857 - 04/27/09 06:21 PM (11 years, 7 months ago)

Just throw a little wedge in.  I don't suggest using more than one or scraping it or shooting solution into your dish.

Any of that is just asking for trouble.  You'll be amazed how well a little wedge will work.  There's just no need to try something foolish to speed it up.


-FF


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