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Invisiblestinkbuttdog
I scat.


Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
Your "dirty" spore print on agar...
    #7780092 - 12/20/07 12:31 AM (16 years, 3 months ago)

does it REALLY matter weather or not your print is dirty if you really know how to work the agar gig? Isolation should lead you to a clear specimen, right? (Provided your environmental conditions are releatively clean?) Just wonderin'.

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Invisiblestinkbuttdog
I scat.


Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
    #7780097 - 12/20/07 12:34 AM (16 years, 3 months ago)

I guess what I'm asking is, can't you pretty much take a big shit on your print and bring it back from the brink of un-usability with careful agar work? I'm really trying to think "yes", I just need your reassurance because you matter to me!:crazy2:

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InvisibleFooMan
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Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
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Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
    #7780111 - 12/20/07 12:41 AM (16 years, 3 months ago)

Sure, but it isn't as easy as it may sound.


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Quick WBS Prep

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Invisiblestinkbuttdog
I scat.


Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
Re: Your "dirty" spore print on agar... [Re: FooMan]
    #7780150 - 12/20/07 12:59 AM (16 years, 3 months ago)

I love you FooManShroom!:grin:

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OfflineanarchOi
Ellenalien is fat.
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Registered: 08/06/07
Posts: 2,293
Loc: ASE Flag
Last seen: 13 years, 1 month
Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
    #7780200 - 12/20/07 01:28 AM (16 years, 3 months ago)

just don't get too excited when you see the white growing
because if your print is dirty, it'll likely be the trich


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Invisiblestinkbuttdog
I scat.


Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
Re: Your "dirty" spore print on agar... [Re: anarchOi]
    #7780204 - 12/20/07 01:31 AM (16 years, 3 months ago)

Quote:

anarchOi said:
just don't get too excited when you see the white growing
because if your print is dirty, it'll likely be the trich




Distinguishable under the microscope.

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OfflineNibin
Getting there
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Registered: 11/29/05
Posts: 4,480
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Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
    #7780381 - 12/20/07 03:41 AM (16 years, 3 months ago)

With patient, careful work with agar you can isolate the good from the bad. But it can take it's time depending on how bad it was.


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Newcomers guide-----> For all things shroomy

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Offlinemebesideme
o-bliminal
Male

Registered: 08/19/04
Posts: 177
Last seen: 7 years, 3 months
Re: Your "dirty" spore print on agar... [Re: Nibin]
    #7780389 - 12/20/07 03:50 AM (16 years, 3 months ago)

As long as your spores are stored in a viable form where they aren't being attacked/eaten without being able to germinate, I sdee no reason you couldn't recover something from pretty much any condition.


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Doot de dooh.... yep yep yep. A signature and stuff...

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InvisibleCrake
Stranger


Registered: 12/04/07
Posts: 194
Re: Your "dirty" spore print on agar... [Re: Nibin]
    #7780392 - 12/20/07 03:56 AM (16 years, 3 months ago)

I think Stinkbutt has made his/her point in case. Indeed, one could actually take a shit on the print... lol, and raise a strong specimen (of bacteria, virus or fungus) from this print.

Agar is the light at the end of the tunnel. I strongly encourage all to invest time and/or money into this facet, and adjacent methodology of cultivation as it propagates an ultimately stronger specimen.


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MANGO

Snowman wakes before dawn. He lies unmoving, listening to the tide coming in, wave after wave sloshing over the various barricades, wish-wash, wish-wash, the rhythm of heartbeat. He would so like to believe he is still asleep.

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Invisiblestinkbuttdog
I scat.


Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
Re: Your "dirty" spore print on agar... [Re: Crake]
    #7780396 - 12/20/07 04:15 AM (16 years, 3 months ago)

Much love,...:D:grin::heart:

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Offlineveda_sticks
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Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
    #7780537 - 12/20/07 06:26 AM (16 years, 3 months ago)

Agar is a recomended method. Prints are not sterile, while it is possible to make a viable syringe from a print, using agar has the advantage that contamination can be seperated from clean myc via transfering to new plates.

As long as you get spores to germinate and grow enough myc to get a even just a tiny peice of tissue. You can transfer to a new plate, and grow it out. Contam shows up?? Transfer again.

There is also the advantage that while you are transfering you can start sectoring and isolating.


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlineveda_sticks
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Re: Your "dirty" spore print on agar... [Re: veda_sticks]
    #7780555 - 12/20/07 06:43 AM (16 years, 3 months ago)

I would like to add that agar also be good for cloning, i have read a few posts here and there were some people just had a hell of a time will LC cloning.

AGAR!!!

yup.

One day, when i buy my own house, i will have my own we lab.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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InvisibleBrainiac
Rogue Scientist
Male


Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋 Flag
Re: Your "dirty" spore print on agar... [Re: veda_sticks]
    #7780701 - 12/20/07 07:58 AM (16 years, 3 months ago)

Some people use brown box cardbored to start spores, MR..


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:Awesketch:

:cool: Fair is Fair :devil:

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OfflineanarchOi
Ellenalien is fat.
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Registered: 08/06/07
Posts: 2,293
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Re: Your "dirty" spore print on agar... [Re: Brainiac]
    #7780903 - 12/20/07 09:26 AM (16 years, 3 months ago)

LCs suck ass for cloning
if you can't get agar
i still highly reccomend the BRF substitute


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
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Loc: Seattle
Last seen: 1 year, 29 days
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Re: Your "dirty" spore print on agar... [Re: anarchOi]
    #7781026 - 12/20/07 10:24 AM (16 years, 3 months ago)

Agar is the gold standard for isolating because the mycelium and contaminants only grow on the surface where they're visible and available for transfer with a scalpel.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineSuchSmartMonkeys
mycologically driven individual
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Registered: 10/26/05
Posts: 1,154
Last seen: 3 years, 21 days
Re: Your "dirty" spore print on agar... [Re: veda_sticks]
    #10195184 - 04/19/09 01:05 PM (14 years, 10 months ago)

Quote:

veda_sticks said:
I would like to add that agar also be good for cloning, i have read a few posts here and there were some people just had a hell of a time will LC cloning.

AGAR!!!

yup.

One day, when i buy my own house, i will have my own we lab.




oh, you don't need a lab...  All you need is a glovebox.  I've done some agar work in some shifty spots with very little contam...  If there's a will, there's a way in this hobby, hehe

and how would you isolate anything with LC?  You just have a bunch of mycelium suspended in liquid, there's no way to tell strains apart from eachother, no way to look for favorable growth, and it seems like it would be tough to make a transfer from a specific colony, even if the colonies were distinguishable...  When an LC has contam in it, it's often times hard to tell the difference between the contam and the healthy mycelium of whatever you're going for.  Has anyone ever used LC for "isolation"?


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